2012 Promega catalogue

Cell Signaling
DNA Quantitation
QuantiFluor dsDNA System
Product Size Cat.# Price ($)
QuantiFluor dsDNA System 1 ml E2670 510.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The QuantiFluor dsDNA System contains a fluorescent
DNA-binding dye that enables sensitive quantitation of small amounts of
double-stranded DNA (dsDNA) in solution. The quantitation of dsDNA is a very
important step in many biological applications, particularly in standard
molecular biology techniques. The dye shows minimal binding to single
stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA.
Features:
• Specificity: Highly specific to dsDNA, minimal binding to ssDNA, RNA,
protein and interfering compounds.
• Sensitivity: Significantly increased sensitivity over absorbance at 260nm
(NanoDrop ® spectrophotometer) for low-concentration samples. Performs
better or equal to PicoGreen ® dye, and has a detection limit of 50pg/ml.
• Ease of Use: System includes all required reagents to quickly set up and
quantitate dsDNA.
• Instrument Compatibility: Easy to set up on both the QuantiFluor and
GloMax ® -Multi Instruments. Also can be used on the Qubit instrument.
Storage Conditions: Store at 4°C.
Protocol Part#
QuantiFluor dsDNA System Technical Manual TM346
Autosomal amplification curves and melt curves from a Plexor ® HY
amplification.
For complete and up-to-date product information visit: www.promega.com/catalog
Plexor ® HY System
Product Size Cat.# Price ($)
Plexor ® HY System 200 reactions DC1001 Pls. Enq.
800 reactions DC1000 Pls. Enq.
DW0991 For Laboratory Use. DC1001, DC1500, DC1000 Not For Medical Diagnostic Use.
Fluorescent
Reporter
iso-dGTP
Dabcyl
iso-dC
Taq
Taq
Primer Annealing
and Extension
Incorporation of
Dabcyl-iso-dGTP
Fluorescence
Quenching
Schematic diagram illustrating the Plexor ® real-time PCR process.
6826TB
4909MA
131
5
DNA and RNA Purification
Section
Contents
Table of
Contents