2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
130
RNasin ® Ribonuclease Inhibitors
Product Size Conc. Cat.# Price ($)
RNasin ® Ribonuclease Inhibitor 2,500 u 20–40 u/µl N2111 Pls. Enq.
Recombinant RNasin ® Ribonuclease
Inhibitor
10,000 u 20–40 u/µl N2115 Pls. Enq.
2,500 u 20–40 u/µl N2511 150.00
10,000 u 20–40 u/µl N2515 507.00
N2511, N2515 For Laboratory Use. N2111, N2115 For Research Use Only. Not for Use in
Diagnostic Procedures.
Description: Natural and Recombinant RNasin ® Ribonuclease Inhibitors have
broad-spectrum RNase inhibitory properties, including the inhibition of eukaryotic
RNases of the neutral type. The 50kDa protein exerts its
inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. The K i value
for the binding of RNasin ® Ribonuclease Inhibitor to RNase (e.g., RNase A) is
approximately 10 –14 M. Promega offers two different preparations: Recombinant
RNasin ® Ribonuclease Inhibitor and Natural RNasin ® Ribonuclease Inhibitor.
Both products are purified using a combination of ion exchange and affinity
chromatography.
Features:
• Inhibits Common Eukaryotic RNases: RNasin ® Ribonuclease Inhibitor
has broad-spectrum RNase inhibitory properties, including RNase A, RNase
B, RNase C and human placental RNase. Does not inhibit RNase T1, S1
nuclease, RNase from Aspergillus, RNase H, RNase ONE Ribonuclease.
• Compatibility: RNasin ® Ribonuclease Inhibitor does not inhibit SP6, T7 or
T3 RNA Polymerase; ImProm-II, AMV or M-MLV Reverse Transcriptase;
or Taq DNA polymerase.
• Broad pH Range: Active over a broad pH range (pH 5–8).
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Protocol Part#
RNasin ® Ribonuclease Inhibitor Product Information 9PIN211
RNasin ® Ribonuclease Inhibitor, Recombinant Protocol 9PIN251
A
B
Percent Inhibition of RNase Activity
RNase A Activity (units)
90
80
70
60
50
40
30
20
10
0
0.25
4u RNasin ®
0.2
0.15
0.1
0.05
0
4u Superase•In
40u Superase•In
400u Superase•In
Amount and Type of Inhibitor Added
RNase A
RNase A + 8u RNasin ®
Ribonuclease Inhibitor
pre-incubation
in assay
RNase A +
8u Superase•In
RNase A +
40u Superase•In
Comparison of RNasin ® Ribonuclease Inhibitor and Superase•In
inhibition of RNase A activity. Panel A. Total yeast RNA assay. Total yeast
RNA was incubated in the presence of 5ng RNase A for 5 minutes at 37°C
in 0.5ml of reaction mix containing 50mM MOPS and 5mM MgCl 2 (pH 6.5).
The indicated amounts of inhibitor (RNasin ® or Superase•In) were mixed
with the RNA prior to RNase addition. After incubation, 0.5ml 10% TCA
was added to stop the reaction and to precipitate the large RNA molecules.
An OD 280 measurement was taken of the TCA-soluble material. Panel B.
“Pre-incubation” and “in assay” conditions. The total yeast RNA assay was
performed as described in Panel A along with an experimental modification
of “pre-incubation.” For the pre-incubation assay, the ribonuclease inhibitors
were mixed with RNase and incubated for 15 minutes at 22°C. The
pre-incubation mix was then added to the RNA.
For complete and up-to-date product information visit: www.promega.com/catalog
3109MA10_0A
3074MA09_0A