2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
128
PolyATtract ® System 1000
Product Size Cat.# Price ($)
PolyATtract ® System 1000 with Magnetic Stand Scalable Z5420 1047.00
PolyATtract ® System 1000 without Magnetic
Stand Scalable Z5400 800.00
PolyATtract ® System 1000 Magnetic Separation
Stand 1 each Z5410 327.00
Available Separately
PolyATtract ® GTC Extraction Buffer 120 ml Z5531 175.00
Z5420, Z5531, Z5400 For Laboratory Use. Z5410 For Research Use Only. Not for Use in
Diagnostic Procedures.
Description: The PolyATtract ® System 1000 isolates messenger RNA directly
from crude cell or tissue lysates, eliminating the need for total RNA
isolations. This system uses the MagneSphere ® technology for the purification
of poly(A)+ RNA, eliminating the need for oligo(dT) cellulose columns. The
increased yield of mRNA using this system allows the detection of low-copynumber
mRNAs in relatively small amounts of material using Northern blot
analysis. The isolated mRNA is suitable for all molecular biology applications,
including in vitro translation, cDNA synthesis, PCR analysis, ribonuclease
(RNase) protection assays, primer extension and Northern blots.
The MagneSphere ® Technology Magnetic Separation Stands can be used in
conjunction with any of the PolyATtract ® Systems and are ideal for
applications requiring multiple paramagnetic isolations of biomolecules.
Features:
• Improved Productivity: mRNA purification directly from tissue or cells in
45 minutes or less. Allows quick collection of magnetic particles.
• Flexibility: Works with tissue amounts from 5mg–2g per isolation.
Magnetic separation stand (Cat.# Z5410) accommodates 1.5ml, 2ml, 15ml
and 50ml tube sizes.
• Convenience: No lengthy ethanol precipitation steps, phenol:chloroform
extractions, or overnight ultracentrifugation through cesium chloride
gradients and lithium chloride (LiCl) precipitations.
Storage Conditions: Store at 4°C. Do not freeze the MagneSphere ®
Paramagnetic Particles.
Protocol Part#
PolyATtract ® System 1000 Technical Manual TM228
MagneSphere ® Magnetic Separation Products Technical Bulletin TB246
PolyATtract ® mRNA Isolation Systems
Product
PolyATtract
Size Cat.# Price ($)
® mRNA Isolation System I (Refill
for Z5200)
PolyATtract
3 isolations Z5210 279.00
® mRNA Isolation System II with
Magnetic Stand
PolyATtract
3 isolations Z5200 374.00
® mRNA Isolation System III with
Magnetic Stand
PolyATtract
15 isolations Z5300 374.00
® mRNA Isolation System IV (Refill
for Z5300)
Available Separately
15 isolations Z5310 279.00
Biotinylated Oligo(dT) Probe (50pmol/µl) 35 µl Z5261 99.00
MagneSphere ® Technology Magnetic
1.5 ml Z5332 134.00
Separation Stand (two-position)
12 × 75 mm Z5333 134.00
Z5210, Z5261, Z5200, Z5300, Z5310 For Laboratory Use. Z5332, Z5333 For Research Use Only.
Not for Use in Diagnostic Procedures.
Description: Cat.# Z5200 contains sufficient reagents for 3 separate mRNA
isolations, each from 1–5mg of total RNA. Cat.# Z5210 contains the same
reagents as Cat.# Z5200, excluding the Magnetic Separation Stand.
Cat.# Z5300 contains sufficient reagents for 15 separate mRNA isolations,
each from 100–1,000μg of total RNA. Cat.# Z5310 contains the same
reagents as Cat.# Z5300, excluding the Magnetic Separation Stand.
The PolyATtract ® mRNA Isolation Systems use the MagneSphere ® technology
to isolate mRNA rapidly and effectively from total RNA. The systems use
a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to
the 3´ poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids
are bound to streptavidin coupled to paramagnetic particles, captured using a
magnetic separation stand and washed at high stringency. The mRNA is eluted
from the solid phase by the simple addition of ribonuclease-free, deionized
water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately
45 minutes. The isolated mRNA is suitable for all molecular biology
applications, including in vitro translation and cDNA synthesis.
Features:
• Improved Productivity: Entire mRNA purification process can be
completed in approximately 45 minutes.
• Highly Pure mRNA: Due to the strength and selectivity of the interaction
between streptavidin and biotin, mRNA bound to the biotinylated oligo(dT)
is captured by streptavidin-coated magnetic particles.
• Confidence in Your Applications: Isolated mRNA is suitable for use with
in vitro translation, RT-PCR and cDNA synthesis.
• Flexibility: Configurations for use with large or small amounts of cells and
tissues.
Storage Conditions: Store at 4°C. Do not freeze the MagneSphere ®
Paramagnetic Particles.
Protocol Part#
PolyATtract ® mRNA Isolation Systems Technical Manual TM021
Streptavidin MagneSphere ® Paramagnetic
Particles
Product Size Conc. Cat.# Price ($)
Streptavidin MagneSphere ® Paramagnetic 9 ml 1 mg/ml Z5481 248.00
Particles
For Laboratory Use.
25 ml 1 mg/ml Z5482 643.00
Description: The Streptavidin MagneSphere ® Paramagnetic Particles (PMPs)
may be used in the magnetic separation or purification of a wide variety of
biotinylated nucleic acid or protein molecules. The particles are quality-tested
and approved for isolation of both nucleic acids and proteins/antibodies.
Features:
• Confidence: The Streptavidin MagneSphere ® Paramagnetic Particles
feature strong, specific binding to biotinylated molecules.
• Improved Purity: Enable binding, washing and magnetic separation from
undesired materials in a solution.
• Flexibility: Applications include purification of DNA, mRNA and proteins.
Storage Conditions: Store at 4°C. Do not freeze the paramagnetic particles.
Protocol Part#
MagneSphere ® Magnetic Separation Products Technical Bulletin TB246
For complete and up-to-date product information visit: www.promega.com/catalog