2012 Promega catalogue

Cell Signaling
Maxwell ® 16 System RNA Purification Kits
Product Size Cat.# Price ($)
Maxwell ® 16 LEV simplyRNA Cells Kit 48 preps AS1270 Pls. Enq.
Maxwell ® 16 LEV simplyRNA Tissue Kit 48 preps AS1280 Pls. Enq.
Maxwell ® 16 Total RNA Purification Kit 48 preps AS1050 Pls. Enq.
Maxwell ® 16 Tissue LEV Total RNA Purification
Kit 48 preps AS1220 Pls. Enq.
Maxwell ® 16 Cell LEV Total RNA Purification Kit 48 preps AS1225 Pls. Enq.
Maxwell ® 16 Viral Total Nucleic Acid
Purification Kit 48 preps AS1150 Pls. Enq.
Available Separately
Maxwell ® 16 Instrument 1 each AS2000 Pls. Enq.
Maxwell ® 16 MDx Instrument
Maxwell
1 each AS3000 Pls. Enq.
® 16 High Strength LEV Magnetic Rod
and Plunger Bar Adaptor 1 each SP1070 Pls. Enq.
AS2000, AS3000 For Laboratory Use. AS1270, AS1280, AS1050, AS1220, AS1225, AS1150 For
Laboratory Use. SP1070 For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Maxwell ® 16 LEV simplyRNA Cells Kit and the Maxwell ® 16
LEV simplyRNA Tissue Kit are for use with the Maxwell ® 16 Instrument configured
with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor. This
RNA purification procedure is a simple method with minimal lysate handling
before automated purification on the Maxwell ® 16 Instrument. The low elution
volume is used to generate concentrated high-quality RNA suitable for use in
downstream applications such as quantitative RT-PCR. The kit provides the
reagents for processing the samples and uses prefilled cartridges for purification,
maximizing simplicity and convenience.
The Maxwell ® 16 Total RNA Purification Kit, Maxwell ® 16 Tissue LEV Total
RNA Purification Kit and Maxwell ® 16 Cell LEV Total RNA Purification Kit are
designed for use with the Maxwell ® 16 Instrument in either the standard or low
elution volume (LEV) configuration. The kits provide high-quality,
essentially DNA-free total RNA using novel approaches to selectively remove
genomic DNA prior to automated RNA purification. You get enhanced sensitivity
and improved confidence in your results for quantitative RT-PCR (qRT-PCR),
RT-PCR, cDNA synthesis and other applications.
For complete and up-to-date product information visit: www.promega.com/catalog
The simple protocols require adding a cleared lysate to the reagent cartridge.
Simply place the reagent cartridge into the Maxwell ® 16 Instrument, and press
start. Purified RNA is obtained in less than 45 minutes of hands-free instrument
operation. No post-purification treatment with nuclease, cleanup or concentration
is required to achieve superior performance in downstream applications.
The Maxwell ® 16 Total RNA Purification Kits are General Purpose Medical
Devices (GPR) in the USA. For up-to-date information visit:
www.promegacom/maxwell16/
Features:
• Enjoy Confidence in Your Application Results: Essentially undetectable
contaminating genomic DNA means fewer repeated experiments and
unexplained or variable results.
• Choose Your Sample Type: Flexibility to purify from tissue, cells, blood
and other samples.
• Achieve High Yield and High Concentration: High yields and highconcentration
total RNA result in better performance in gene expression
analysis applications.tions.
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
No detectable cross-contamination. Sixteen purification reactions were performed
using an input of 25mg of mouse liver lysate (odd lanes) or SV RNA Lysis Buffer alone
(even lanes). Panel A. Four-microliter aliquots of each purified sample were resolved by
1.2% agarose gel electrophoresis under denaturing conditions. Lane M, RNA Markers
(Cat.# G3191). Panel B. Equivalent volumes (1μl) of each sample were amplified by
endpoint RT-PCR using a primer pair specific for a portion of beta actin RNA. A total
of five microliters of each amplification reaction was analyzed by 1.2% agarose gel
electrophoresis and visualized by ethidium bromide staining. Lane M, 1kb DNA Ladder
(Cat.# G5711).
TISSUE
simply RNA
CELLS
kidney brain heart lung spleen liver HEK293 HeLa Jurkat
5967TB
28S
18S
Intact RNA Extracted from Tissue Using the Maxwell ® 16 LEV
simplyRNA Tissue Kit. Extracted tissue samples were run on a FlashGel ®
System for 5 minutes and signal developed for 15 minutes. The 28S, 18S and
5S are clearly visible indicating intact RNA.
10001TA
5S
127
5
DNA and RNA Purification
Section
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