2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
126
RNAgents ® Denaturing Solution
Product Size Cat.# Price ($)
RNAgents ® Denaturing Solution 120 ml Z5651 201.00
For Laboratory Use.
Description: RNAgents ® Denaturing Solution lyses cells or tissue under
conditions that rapidly inhibit ribonucleases, using two potent inhibitors of
RNase, guanidine thiocyanate and β-mercaptoethanol. The RNAgents ®
Denaturing Solution is designed to be used in concert with acidic
phenol:chloroform and alcohol (isopropanol) for purification of total RNA.
Storage Conditions: Store at 4°C.
SV 96 Total RNA Isolation System
Product Size Cat.# Price ($)
SV 96 Total RNA Isolation System 1 × 96 each Z3500 499.00
Available Separately
5 × 96 each Z3505 1982.00
RNA Lysis Buffer (RLA) 50 ml Z3051 67.00
RNA Wash Solution (RWA) 58.8 ml Z3091 67.00
Nuclease-Free Water 150 ml P1195 125.00
Wizard ® SV 96 Binding Plates 10 pack A2271 1450.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The SV 96 Total RNA Isolation System provides a high-throughput
technique to prepare intact RNA from tissue and cultured cells. Total RNA can
be purified from 96 samples in less than an hour without centrifugation. The
system also incorporates a DNase treatment step that is designed to
substantially reduce genomic DNA contamination, which can interfere
with amplification-based methodologies. Purification is achieved without
phenol:chloroform extraction or ethanol precipitation, and there is no detectable
DNase carryover in the final RNA preparation.
Protocols are available for Beckman Coulter and PerkinElmer instruments.
Features:
• Confidence in Results: The product is tested to ensure that purified RNA
will perform optimally in RT-PCR.
• Unique Design: Novel vacuum manifold eliminates waste handling. Novel
plate design prevents cross-contamination during sample processing.
• Flexibility: The system is designed for both manual and automated
formats.
• Automation: Validated automated methods available at:
www.promega.com/automethods/
• Your Choice of Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store the SV RNA Lysis Buffer with β-Mercaptoethanol
(BME) added at 4°C. Store all other components at 22–25°C.
Protocol Part#
SV 96 Total RNA Isolation System Technical Bulletin TB294
MagneSil ® Total RNA mini-Isolation System
Product Size Cat.# Price ($)
MagneSil ® Total RNA mini-Isolation System
For Research Use Only. Not for Use in Diagnostic Procedures.
4 plate Z3351 Pls. Enq.
Description: The MagneSil ® Total RNA mini-Isolation System provides a
high-throughput 96-well format for fast, simple preparation of intact total RNA
from small amounts of cell culture (≤1 × 10 5 tissue culture cells), tissue (≤2mg
tissue lysate in 100μl) or freshly isolated whole blood (≤20μl). The protocol
enables high-throughput automated purification on a variety of liquid-handling
workstations. Isolation of total RNA in a 384-well format from cell culture
(≤1 × 10 3 cells) and freshly isolated whole blood (≤5μl) also may be performed.
Total RNA purification is achieved without vacuum filtration, centrifugation or
precipitation. The 96-well total RNA isolation procedure takes about 30 minutes
to complete using a liquid-handling workstation.
Total RNA purified using this system is suitable for a variety of molecular
biology applications including endpoint RT-PCR amplification and real-time
RT-PCR.
Features:
• Improve Productivity: Only 30 minutes are required to process one
96-well plate, or 50 minutes for one 384-well plate on a Beckman Coulter
Biomek ® FX liquid handler.
• Improve Real-Time PCR Performance: Elution volumes as low as 15μl
provide concentrated RNA without the need for time-consuming vacuum
concentration.
• Gain Confidence in Results: DNase I treatment is included to remove
genomic DNA contamination.
• Achieve Convenience: Robotic protocols require no user intervention
once you start the automated robotic method.
• Automate This Assay: Validated automated methods are available at:
www.promega.com/automethods/
Storage Conditions: Store at 22–25°C.
Protocol Part#
MagneSil ® Total RNA mini-Isolation System Technical Bulletin TB328
MagaZorb ® Total RNA Mini-Prep Kit
Product Size Cat.# Price ($)
MagaZorb ® Total RNA Mini-Prep Kit 200 preps MB2004 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The MagaZorb ® RNA Kit provides an easy, fast and
cost-effective technique for isolating PCR-quality total RNA. Using one simple
protocol, a high yield of purified total RNA can be isolated from various sources
including whole blood (fresh or citrate-, heparin- or EDTA-treated), buffy coat,
leukocytes and tissue (fresh or frozen).
Features:
• Convenient: Contains all needed reagents so that no reagent preparation
is required.
• Efficient: Eliminates the need for centrifugation, vacuum filtration or
column separation, increasing sample throughput and improving
reproducibility.
• Safe: Does not require organic solvents, eliminating the need for special
storage or waste disposal.
Storage Conditions: Store at 22–25°C.
Protocol Part#
MagaZorb ® Total RNA Mini-Prep Kit Technical Bulletin TB378
For complete and up-to-date product information visit: www.promega.com/catalog