2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
RNA Purification
124
SV Total RNA Isolation System
Product Size Cat.# Price ($)
SV Total RNA Isolation System 10 preps Z3101 Pls. Enq.
50 preps Z3100 298.00
250 preps Z3105 1288.00
Available Separately
Red Blood Cell Lysis Solution (CLB) 200 ml Z3141 82.00
RNA Lysis Buffer (RLA) 50 ml Z3051 67.00
Z3101, Z3141, Z3100, Z3105 For Laboratory Use. Z3051, Z3380, Z3382, Z3384 For Research
Use Only. Not for Use in Diagnostic Procedures.
Description: The SV Total RNA Isolation System provides a fast and simple
technique for preparation of intact total RNA from tissues, cultured cells and
white blood cells in as little as one hour. Using this membrane-based
purification system, up to 60mg of tissue can be processed per purification,
depending on tissue type. The system incorporates a DNase treatment step
directly on the minicolumn membrane. This step substantially reduces genomic
DNA contamination, which can interfere with amplification-based
methodologies. Purification is achieved without the use of phenol:chloroform
extractions or ethanol precipitations, and there is no DNase carryover in the
final RNA preparation.
Features:
• Safety and Efficiency: Rapid isolation of high yields of total RNA without
the use of hazardous compounds like phenol.
• Flexibility: Single system for isolation directly from blood, cells or
tissue. Two methods available for purification: microcentrifugation (spin) or
vacuum.
• Confidence: Purified RNA suitable for all routine molecular biology
applications, including RT-PCR and Northern blotting.
Storage Conditions: Store at 22–25°C.
Protocol Part#
SV Total RNA Isolation System Protocol TM048
bp
1,500 –
1,000 –
700 –
400 –
β-actin APC
M 5µl 5µl 10µl 15µl
RNA was isolated from 1ml of human blood using the SV Total RNA
Isolation System. RT-PCR was performed using the indicated volumes
of eluted RNA and primers complementary to human β-actin or human
adenomatous polyposis coli (APC) gene with the Access RT-PCR System
(Cat.# A1250). Lane M = 100bp DNA Ladder (Cat.# G2101).
Average Yields of Total RNA Isolated Using SV Total RNA Isolation
System.
Maximum Avg. Yield Avg. Yield
Amt. to per Prep per mg
Samples Process (µg) Tissue (µg) A 260/A 280
Mouse Tissues
Liver 30mg 131 4.4 1.9
Kidney 20mg 44 2.2 1.9
Spleen 15mg 79 5.3 1.9
Brain 60mg 39 0.65 2.1
Muscle 30mg 22 0.73 2.1
Rat Tissues
Pancreas 30mg 100 3.5 1.9
Heart 60mg 16 0.27 2.1
Lung 60mg 36 0.6 2.1
Bacteria
E. coli 1 × 10 9 cells 36 N/A 2.0
Yeast
S. cerevisiae 4 × 10 7 cells 19 N/A 2.1
Plant
Tomato Leaf 30mg 4.6 0.15 2.0
Cell Line
RAW264.7 5 × 10 6 cells 51 N/A 2.1
N/A = Not applicable 9485LA
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