2012 Promega catalogue

Cell Signaling
Wizard ® MagneSil ® Plasmid Purification
System
Product Size Cat.# Price ($)
Wizard ® MagneSil ® Plasmid Purification
System
4 × 96 preps A1630 676.00
8 × 96 preps A1631 1223.00
Wizard ® MagneSil ® Plasmid Purification
System, HTP1 100 × 96 preps A1635 Pls. Enq.
Available Separately
MagneSil ® RED 100 ml A1641 1333.00
MagneSil ® BLUE 100 ml A2201 951.00
Cell Resuspension Solution 500 ml A7114 151.00
Cell Lysis Solution 500 ml A7124 151.00
Neutralization Solution 500 ml A7132 140.00
Elution Buffer 500 ml A1655 110.00
Collection Plates (4-pack)
For Research Use Only. Not for Use in Diagnostic Procedures.
1 each A9161 24.00
Description: The Wizard ® MagneSil ® Plasmid DNA Purification System provides
a simple and reliable method for the rapid isolation of plasmid DNA in a
96-well, high-throughput format. The purified plasmid can be used directly for
automated fluorescent sequencing, such as with BigDye ® terminator
sequencing chemistry, as well as for other standard molecular biology
techniques including restriction enzyme digestion.
The use of the MagneSil ® Paramagnetic Particles for lysate clearing (BLUE) as
well as DNA capture (RED) circumvents the need for centrifugation or vacuum
manifolds, making the system ideal for full automation on a Beckman Coulter
or Tecan instrument.
Features:
• Improve Productivity: Process multiple plates without user intervention.
• Gain Confidence: Consistent performance in fluorescent sequencing
reactions.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at 22–25°C.
Protocol Part#
TB286
Wizard ® MagneSil ® Plasmid DNA Purification System Technical
Bulletin
Accuracy (%)
100
98
96
94
92
90
500 600 700
Bases
2 O.D.
4 O.D.
6 O.D.
8 O.D.
10 O.D.
12 O.D.
Accuracy by read length for plasmid DNA sequenced following
isolation using Wizard ® MagneSil ® Plasmid Purification System.
Results demonstrate >700 consecutive bases analyzed with >98% accuracy
of base identity.
For complete and up-to-date product information visit: www.promega.com/catalog
2890MA03_0A
Wizard MagneSil Tfx System
Product Size Cat.# Price ($)
Wizard MagneSil Tfx System
Available Separately
4 × 96 preps A2380 1088.00
Endotoxin Removal Resin 100 ml A2191 1165.00
4/40 Wash Solution 115 ml A2221 407.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Wizard MagneSil Tfx System provides a simple and
reliable method for the rapid isolation of transfection-quality plasmid DNA in a
96-well, high-throughput format. The use of MagneSil ® Paramagnetic Particles
for lysate clearing as well as DNA capture circumvents the need for centrifugation
or vacuum manifolds, allowing DNA purification with the Wizard MagneSil
Tfx System to be completely automated.
An automated method has been developed for use of this product with a
Beckman Coulter Biomek ® FX robotic workstation. This procedure requires
approximately 45 minutes to process a single 96-well plate. The method
can be adapted to other robotic workstations, such as the Beckman Coulter
Biomek ® 2000 or the Tecan Genesis ® instrument.
Features:
• Improve Transfection Results: Use of Endotoxin Removal Resin cuts
endotoxin carryover as much as 95% over standard sequencing-grade
automated plasmid systems.
• Enhance Mammalian Protein Expression: Three- to fivefold increase
in protein expression compared to plasmid isolated from an automated
sequencing-grade plasmid purification system.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at 22–25°C.
Protocol Part#
Wizard MagneSil Tfx System Technical Bulletin TB314
% of Ultra RLU
160
140
120
100
80
60
40
20
0
Transfection Results
Wizard MagneSil Tfx
Qiagen Turbo
Qiagen Ultra
3T3 CV-1 HeLa 293s CHO
A comparison of transfection efficiencies for different DNA
purification systems with various cell lines. A variety of eukaryotic cell
lines were transfected with pGL3-Control Vector purified using the Wizard
MagneSil Tfx System or Qiagen Turbo or Ultra systems. Transfection
efficiency was determined by measuring firefly luciferase luminescence, and
the results were normalized to those for the Qiagen Ultra system.
3907MA12_2A
123
5
DNA and RNA Purification
Section
Contents
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