2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
120<br />
Wizard ® Plus Minipreps DNA Purification<br />
Systems<br />
Product Size Cat.# Price ($)<br />
Wizard ® Plus Minipreps DNA Purification System 50 preps A7100 220.00<br />
100 preps A7500 398.00<br />
250 preps A7510 831.00<br />
Available Separately<br />
Cell Resuspension Solution (CRA) 150 ml A7112 75.00<br />
Cell Lysis Solution (CLA) 150 ml A7122 75.00<br />
Neutralization Solution (NSA) 150 ml A7131 106.00<br />
Column Wash Solution (CWB) 125 ml A8102 88.00<br />
Wizard ® Minipreps DNA Purification Resin 250 ml A7141 351.00<br />
Wizard ® Minicolumns 250 each A7211 351.00<br />
A7112, A7122 For Laboratory Use. A7100, A7500, A7131, A7510, A8102, A7141, A7211 For<br />
Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The resin-based Wizard ® Plus Minipreps DNA Purification<br />
System provides a simple and reliable method for rapid isolation of plasmid<br />
DNA. When using the standard protocol, the entire miniprep process can<br />
be completed in 15 minutes or less, with no organic extractions or ethanol<br />
precipitations. Minipreps may be processed individually or in multiples with<br />
the Vac-Man ® (20-sample capacity, Cat.# A7231) or Vac-Man ® Jr. (2-sample<br />
capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the<br />
Wizard ® Minicolumn in Nuclease-Free Water (Cat.# P1193). The purified<br />
plasmid can be used directly for automated fluorescent DNA sequencing and<br />
restriction digestion without further manipulation and also can be used for in<br />
vitro transcription reactions supplemented with a ribonuclease inhibitor, such as<br />
Recombinant RNasin ® Ribonuclease Inhibitor (Cat.# N2511).<br />
The Wizard ® Minipreps DNA Purification Resin is used in the isolation and<br />
preparation of plasmid DNA in conjunction with the Wizard ® Plus Minipreps<br />
DNA Purification Systems. The resin is available with the systems and as a<br />
standalone product.<br />
Features:<br />
• High Performance: DNA is suitable for most molecular biology<br />
applications, including fluorescent sequencing.<br />
• Confidence in Results: Purified DNA meets a target of >98% accuracy<br />
over 500 bases using pGEM ® -3Zf(+) Vector in BigDye ® terminator<br />
sequencing.<br />
• Fast: Entire procedure may be completed in 15 minutes or less.<br />
• Convenient: No phenol extractions or ethanol precipitations required.<br />
Storage Conditions: Store at 22–25°C.<br />
Protocol Part#<br />
Wizard ® Plus Minipreps DNA Purification System Technical Bulletin TB117<br />
Wizard ® Plus Midipreps DNA Purification<br />
System<br />
Product Size Cat.# Price ($)<br />
Wizard ® Plus Midipreps DNA Purification System<br />
Available Separately<br />
25 preps A7640 408.00<br />
Cell Resuspension Solution (CRA) 150 ml A7112 75.00<br />
Cell Lysis Solution (CLA) 150 ml A7122 75.00<br />
Neutralization Solution (NSA) 150 ml A7131 106.00<br />
Column Wash Solution (CWB) 125 ml A8102 88.00<br />
Wizard ® Midipreps DNA Purification Resin 1,000 ml A7701 1183.00<br />
Wizard ® Midicolumns 100 each A7651 363.00<br />
A7112, A7122 For Laboratory Use. A7640, A7131, A8102, A7701, A7651 For Research Use<br />
Only. Not for Use in Diagnostic Procedures.<br />
Description: The resin-based Wizard ® Plus Midipreps DNA Purification<br />
System provides a simple and reliable method for rapid isolation of plasmid<br />
DNA. When using the standard protocol, the entire midiprep process can be<br />
completed in 90 minutes or less, yielding up to 200μg of high-quality DNA<br />
with no organic extractions or ethanol precipitations. Multiple midipreps can be<br />
easily processed at one time with the Vac-Man ® (20-sample capacity, Cat.#<br />
A7231) or Vac-Man ® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum<br />
Manifold. DNA is eluted from the Wizard ® Midicolumn in Nuclease-Free Water<br />
(Cat.# P1193). The purified plasmid can be used directly for automated<br />
fluorescent DNA sequencing or restriction digestion without further<br />
manipulation and also can be used for in vitro transcription reactions<br />
supplemented with a ribonuclease inhibitor such as Recombinant RNasin ®<br />
Ribonuclease Inhibitor (Cat.# N2511). The system includes sufficient reagents<br />
for 25 DNA isolations from 10–100ml of liquid culture.<br />
Features:<br />
• Fast: Rapid batch column method used for DNA isolation.<br />
• Safe: Eliminates the need for cesium chloride:ethidium bromide gradient<br />
centrifugation and does not require organic extractions.<br />
• Reliable: Yields plasmid DNA of comparable quantity and quality to cesium<br />
chloride:ethidium bromide gradient techniques that are much more timeand<br />
labor-intensive.<br />
• High Performance: DNA is suitable for restriction enzyme digestions,<br />
automated fluorescent DNA sequencing, transformation and subcloning.<br />
• Confidence in Results: Purified DNA meets a target of >98% accuracy<br />
over 500 bases using pGEM ® -3Zf(+) Vector in BigDye ® terminator<br />
sequencing.<br />
Storage Conditions: Store at 22–25°C.<br />
Protocol Part#<br />
Wizard ® Plus Midipreps DNA Purification System Technical Bulletin TB173<br />
For complete and up-to-date product information visit: www.promega.com/catalog
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