2012 Promega catalogue

Cell Signaling
PureYield Plasmid Maxiprep System
Product Size Cat.# Price ($)
PureYield Plasmid Maxiprep System 10 preps A2392 305.00
25 preps A2393 713.00
Available Separately
Eluator Vacuum Elution Device 4 each A1071 77.00
A1071 For Laboratory Use. A2392, A2393, A6730, A6732, A6734 For Research Use Only. Not
for Use in Diagnostic Procedures.
Description: The PureYield Plasmid Maxiprep System isolates transfectionquality
plasmid DNA. The system provides a rapid method for purification of up
to 1mg of plasmid DNA from a 250ml bacterial culture. Plasmid DNA can be
purified rapidly with the vacuum protocol, greatly reducing the time spent on
purification compared to silica resin or other membrane-column methods.
The PureYield Plasmid Maxiprep System incorporates a unique Endotoxin
Removal Wash designed to remove substantial amounts of protein, RNA and
endotoxin contaminants from purified plasmid DNA. Removal of contaminants
improves the robustness of sensitive applications such as eukaryotic
transfection, in vitro transcription and coupled in vitro transcription/translation
(e.g., TnT ® Quick Coupled Transcription/Translation System). Purification is
achieved without isopropanol precipitation of purified plasmid DNA.
The system has been designed for use with a vacuum source and vacuum
manifold (e.g., the Vac-Man ® Laboratory Vacuum Manifold).
The Eluator Vacuum Elution Device is used to elute nucleic acids from
PureYield Midiprep or Maxiprep columns. It consists of two pieces, a
blue base and a clear column assembly. The base interfaces with a Vacuum
Manifold that contains Luer-Lok ® fittings, such as the Vac-Man ® Laboratory
Vacuum Manifold (Cat.# A7231), and holds a 1.5ml tube to capture the eluted
nucleic acids. The column assembly accepts PureYield Midiprep or Maxiprep
columns.
The Eluator Device eliminates the requirement for a centrifuge with a
swinging bucket rotor for nucleic acid purification, simplifying and speeding
purification protocols.
Features:
• Improved Productivity: Vacuum protocol simplifies purification of multiple
samples at one time.
• Confidence in Results: High purity and concentration of plasmid DNA
gives proven performance in transfection, in vitro expression and other
molecular biology applications.
• Ease of Use: Simple protocol eliminates tedious, gravity-drip columns and
post-elution alcohol precipitation.
• Flexibility: PureYield membrane column allows purification of large
amounts of plasmid DNA, exceeding the capabilities of other maxiprep
systems.
Storage Conditions: Store at 22–25°C.
Protocol Part#
PureYield Plasmid Maxiprep System Technical Manual TM280
Maxiprep
For complete and up-to-date product information visit: www.promega.com/catalog
PureYield Done in 60 Minutes
Wizard ® 240 Minutes
Qiagen 240 Minutes
Comparison of time required per prep using different systems.
PureYield system protocols were performed according to the instructions
using 250ml overnight cultures of JM109 bacteria transformed with a
high-copy-number plasmid. Total times to perform the PureYield preps are
noted. Other times are estimated based on protocols.
Plasmid Amount (µg)
1,200
1,000
800
600
400
200
0
Standard Midi
Alternative Midi
Standard Maxi
50ml 100ml 150ml 200ml 250ml
Bacterial Culture (ml)
Plasmid yield from various culture volumes using the PureYield
Maxiprep and Midiprep Systems. Increasing amounts of JM109 containing
the phMGFP plasmid were grown and processed using the PureYield
Plasmid Systems. Lysate was prepared using the midiprep standard vacuum
protocol, the midiprep alternative lysate clearing protocol and the standard
maxiprep protocol. The midiprep standard protocol is recommended only for
50ml cultures.
5815MA
5816MA
119
5
DNA and RNA Purification
Section
Contents
Table of
Contents