2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
118<br />
PureYield Plasmid Midiprep System<br />
Product Size Cat.# Price ($)<br />
PureYield Plasmid Midiprep System 25 preps A2492 310.00<br />
100 preps A2495 1156.00<br />
300 preps A2496 Pls. Enq.<br />
Available Separately<br />
Cell Resuspension Solution (CRA) 315 ml A7115 107.00<br />
Cell Lysis Solution (CLA) 315 ml A7125 107.00<br />
Neutralization Solution (NSB) 500 ml A1485 92.00<br />
Eluator Vacuum Elution Device 4 each A1071 77.00<br />
A1071 For Laboratory Use. A2492, A7115, A2495, A7125, A1485, A2496, A6740, A6742, A6744<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The PureYield Plasmid Midiprep System is designed to isolate<br />
transfection-quality plasmid DNA. The system provides a rapid method for<br />
purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid<br />
DNA can be purified in as little as 30 minutes with the vacuum protocol,<br />
greatly reducing the time spent on purification compared to silica resin or other<br />
membrane-column methods. An alternative protocol allows purification of over<br />
400μg of high-copy-number plasmid from 250ml of E. coli culture.<br />
The PureYield Plasmid Midiprep System incorporates a unique Endotoxin<br />
Removal Wash designed to remove substantial amounts of protein, RNA and<br />
endotoxin contaminants from purified plasmid DNA. Removal of contaminants<br />
improves the robustness of sensitive applications such as eukaryotic<br />
transfection, in vitro transcription and coupled in vitro transcription/translation<br />
(e.g., TnT ® Quick Coupled Transcription/Translation System). Purification is<br />
achieved without isopropanol precipitation of purified plasmid DNA or extensive<br />
centrifugation, providing rapid purification from a single method.<br />
The system has been designed for use with centrifugation or vacuum (e.g., the<br />
Vac-Man ® Laboratory Vacuum Manifold).<br />
The Eluator Vacuum Elution Device is used to elute nucleic acids from<br />
PureYield Midiprep or Maxiprep columns. It consists of two pieces, a<br />
blue base and a clear column assembly. The base interfaces with a Vacuum<br />
Manifold that contains Luer-Lok ® fittings, such as the Vac-Man ® Laboratory<br />
Vacuum Manifold (Cat.# A7231), and holds a 1.5ml tube to capture the eluted<br />
nucleic acids. The column assembly accepts PureYield Midiprep or Maxiprep<br />
columns.<br />
The Eluator Device eliminates the requirement for a centrifuge with a<br />
swinging bucket rotor for nucleic acid purification, simplifying and speeding<br />
purification protocols.<br />
Features:<br />
• Improved Productivity: Vacuum protocol allows plasmid DNA purification<br />
in as little as 30 minutes.<br />
• Confidence in Results: High purity and concentration of plasmid DNA<br />
gives proven performance in transfection, in vitro expression and other<br />
molecular biology applications.<br />
• Ease of Use: Simple protocol eliminates tedious high-speed centrifugation,<br />
gravity-drip columns, and post-elution alcohol precipitation.<br />
• Flexibility: PureYield membrane column allows purification of large<br />
amounts of plasmid DNA, exceeding the capabilities of other midiprep<br />
systems.<br />
Storage Conditions: Store all system components at 22–25°C.<br />
Protocol Part#<br />
PureYield Plasmid Midiprep System Technical Manual TM253<br />
Midiprep<br />
PureYield Done in 30 Minutes<br />
Wizard ® 99 Minutes<br />
Qiagen 200 Minutes<br />
Comparison of time required per midiprep for different systems. Each<br />
system protocol was performed according to the manufacturer’s instructions<br />
using 50ml of an overnight culture of JM109 bacteria transformed with highcopy-number<br />
plasmid [pGEM ® -3Zf(+) Vector, Cat.# P2271]. Total time to<br />
perform midiprep is noted.<br />
PureYield Plasmid Midiprep System. Proper assembly of Lysate<br />
Clearing Column (blue) and DNA Binding Column (white) for use with<br />
the DNA Purification by Vacuum protocol.<br />
Luminescence (RLU)<br />
8,000<br />
7,000<br />
6,000<br />
5,000<br />
4,000<br />
3,000<br />
2,000<br />
1,000<br />
0<br />
Qiagen Eppendorf PureYield<br />
System<br />
DNA Preparation Methods<br />
Comparison of transfection of plasmid DNA purified with the<br />
PureYield System and other midiprep systems. psiCHECK-2 Vector<br />
(Cat.# C8021), which carries a firefly luciferase gene, was isolated from E. coli<br />
using the PureYield System, the Qiagen HiSpeed ® Plasmid Midi Kit or the<br />
Eppendorf Perfectprep ® Plasmid Midi Kit. HeLa cells were transfected using<br />
0.07μg of DNA in a total of 25μl. The firefly luciferase signal was monitored<br />
with the Dual-Glo Luciferase Assay System (Cat.# E2920).<br />
For complete and up-to-date product information visit: www.promega.com/catalog<br />
4587MB<br />
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