2012 Promega catalogue

Cell Signaling
Plasmid Purification
Wizard ® Plus SV Minipreps DNA Purification
Systems
Product Size Cat.# Price ($)
Wizard ® Plus SV Minipreps DNA Purification
System
Wizard ® Plus SV Minipreps DNA Purification
System + Vacuum Adapters
50 preps A1330 89.00
250 preps A1460 400.00
1,000 preps A1465 1450.00
50 preps A1340 143.00
250 preps A1470 581.00
Available Separately
Alkaline Protease Solution 3 ml A1441 66.00
Vacuum Adapters 20 each A1331 33.00
A1465 For Laboratory Use. A1330, A1441, A1331, A1460, A1340, A1470, A6760, A6762, A6764
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Wizard ® Plus SV Minipreps DNA Purification System, a silica
membrane-based system, provides a simple and reliable method for rapid
isolation of plasmid DNA. The entire miniprep procedure can be completed in
45 minutes or less, depending on the number of samples processed. Using the
system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture.
The purified plasmid DNA can be used directly for automated fluorescent
BigDye ® terminator DNA sequencing as well as for other standard molecular
biology techniques without further manipulation. It also can be used for in vitro
transcription reactions when supplemented with a ribonuclease inhibitor such
as Recombinant RNasin ® Ribonuclease Inhibitor (Cat.# N2511).
Features:
• Improved Productivity: 20 minipreps processed in less than 45 minutes.
• High Performance: 1–20μg of high-quality plasmid DNA, enough for
multiple applications.
• Safety and Convenience: No phenol extractions or precipitations
required.
• Flexibility: Choice of spin (microcentrifuge) or vacuum purification
formats.
• Consistent Quality: Alkaline protease step improves plasmid quality.
• Confidence in Results: Purified DNA meets a target of >98% accuracy
over 500 bases using pGEM ® -3Zf(+) Vector in BigDye ® terminator
sequencing.
Storage Conditions: Store at 22–25°C.
Protocol Part#
Wizard ® Plus SV Minipreps DNA Purification System
Technical Bulletin
TB225
1612TA09_6A
1 2 3 4 5 6 M
kb
– 10.0
––––– 8.0
– 6.0
––––– 5.0
– 4.0
– 3.0
––––– 2.5
– 2.0
– 1.5
– 1.0
– 0.75
– 0.50
– 0.25
High-quality restriction digests using plasmid purified with the
Wizard ® Plus SV Minipreps DNA Purification System. High-copy
pGEM ® -3Zf(+) Vector (lanes 1–3) and low-copy pALTER ® -1 Vector (lanes 4–6)
were each digested in two separate Promega restriction enzyme reactions. All
lanes show reproducible and efficient cutting of the plasmid DNA. Lane M is
the 1kb DNA Ladder (Cat.# G5711).
For complete and up-to-date product information visit: www.promega.com/catalog
pGEM ® -3Zf(+) plasmid sequenced with the T7 Promoter Primer (Cat.#
Q5021) using DNA purified with the Wizard ® Plus SV Minipreps DNA
Purification System.
PureYield Plasmid Miniprep System
Product Size Cat.# Price ($)
PureYield Plasmid Miniprep System 100 preps A1223 257.00
250 preps A1222 573.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The PureYield Plasmid Miniprep System is designed to rapidly
isolate highly pure plasmid DNA. The system provides a rapid method for
purification of up to 15μg of plasmid DNA from 600μl to 3ml of bacteria
culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield
Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash
designed to remove substantial amounts of protein, RNA and endotoxin
contaminants from purified plasmid DNA. Removal of contaminants improves
the robustness of sensitive applications such as eukaryotic transfection, in vitro
transcription and coupled in vitro transcription/translation (e.g., TnT ® Quick
Coupled Transcription/Translation System). Purification is achieved without
isopropanol precipitation of purified plasmid DNA or extensive centrifugation,
providing rapid purification from a single method.
The system has been designed for use with centrifugation or vacuum (e.g., the
Vac-Man ® Laboratory Vacuum Manifold).
Features:
• Improved Productivity: Rapid protocol purifies plasmid DNA in
10 minutes.
• Robust Performance: High purity and concentration of plasmid DNA
gives proven performance in transfection, cell-free expression and other
molecular biology applications.
• Confidence in Results: Lysis/neutralization indicator dye ensures
success every time.
• Flexible: Centrifugation and vacuum protocols are available.
Storage Conditions: Store all system components at 22–25°C.
Protocol Part#
PureYield Plasmid Miniprep System Technical Bulletin TB374
117
5
DNA and RNA Purification
Section
Contents
Table of
Contents