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2012 Promega catalogue

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Cell Signaling<br />

MagneSil ® KF, Genomic System<br />

Product Size Cat.# Price ($)<br />

MagneSil ® KF, Genomic System 200 preps MD1460 664.00<br />

Available Separately<br />

MagneSil ® KF, Paramagnetic Particles 40 ml MD1471 Pls. Enq.<br />

Lysis Buffer, KF<br />

For Laboratory Use.<br />

160 ml MD1521 174.00<br />

Description: The MagneSil ® KF, Genomic System is designed for easy,<br />

walkaway, low- to moderate-throughput automated genomic DNA purification<br />

from blood and other samples. For blood samples, lysis occurs concurrently<br />

with DNA binding to MagneSil ® Paramagnetic Particles. After washes to<br />

remove heme and proteins, purified genomic DNA is ready for PCR and other<br />

downstream applications. The system is designed to purify 2–6μg of genomic<br />

DNA from 200μl of anti-coagulated liquid blood.<br />

The MagneSil ® KF, Genomic System is designed to run on the Thermo Electron<br />

KingFisher ® mL instrument, which automates DNA purification in a flexible<br />

1- to 15-sample batch, 25-minute walkaway format. The compact size of<br />

the KingFisher ® mL can be used on the benchtop or in a laminar flow hood.<br />

Please contact Thermo Electron for more information on the KingFisher ® mL<br />

instrument.<br />

Features:<br />

• Improve Productivity: Use automated 25-minute optimized, walkaway<br />

protocol with no training. Eliminate laborious manual methods.<br />

• Rely On a Performance-Tested System: Purified DNA is tested in PCR,<br />

multiplex PCR, fluorescent STR analysis and SNP genotyping applications.<br />

• Conserve Valuable Lab Space: The small footprint (30 × 30 × 30cm)<br />

of the Thermo Electron KingFisher ® mL instrument delivers automated<br />

throughput that makes sense for smaller labs. No external PC required.<br />

• Automate This Assay: Validated automated methods available at:<br />

www.promega.com/automethods/<br />

Storage Conditions: Store at 22–25°C. Do not freeze the MagneSil ® KF<br />

Paramagnetic Particles.<br />

Protocol Part#<br />

MagneSil ® KF, Genomic System Technical Bulletin TB322<br />

Wizard ® Magnetic 96 DNA Plant System<br />

Product Size Cat.# Price ($)<br />

Wizard ® Magnetic 96 DNA Plant System 2 × 96 preps FF3760 579.00<br />

4 × 96 preps FF3761 1121.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

For additional information see page 180.<br />

For complete and up-to-date product information visit: www.promega.com/catalog<br />

MagaZorb ® DNA Mini-Prep Kit<br />

Product Size Cat.# Price ($)<br />

MagaZorb ® DNA Mini-Prep Kit 200 preps MB1004 Pls. Enq.<br />

800 preps MB1008 Pls. Enq.<br />

Available Separately Size Conc. Cat.# Price ($)<br />

Proteinase K (PK) Solution 16 ml 20 mg/ml MC5008 Pls. Enq.<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

Description: The MagaZorb ® DNA Kit provides an easy, fast and cost-effective<br />

technique for isolating PCR-quality DNA. Using one simple protocol, a high<br />

yield of purified DNA can be isolated from a wide variety of sources including<br />

whole blood (fresh or frozen, citrate-, heparin- or EDTA-treated), buffy coat,<br />

leukocytes, milk, seminal fluid, dried blood spots, cultured cells, tissue (fresh,<br />

frozen or formalin-fixed paraffin-embedded), saliva, urine, stool, hair, buccal<br />

swabs and vaginal swabs.<br />

Features:<br />

• Convenient: Contains all needed reagents; no reagent preparation is<br />

required.<br />

• Efficient: Eliminates centrifugation, vacuum filtration or column<br />

separation, increasing sample throughput and improving reproducibility.<br />

• Safe: Does not require organic solvents, eliminating the need for special<br />

storage or waste disposal.<br />

Storage Conditions: Store at 22–25°C.<br />

Protocol Part#<br />

MagaZorb ® DNA Mini-Prep Kit Technical Bulletin TB376<br />

Wizard ® Magnetic DNA Purification System<br />

for Food<br />

Product Size Cat.# Price ($)<br />

Wizard ® Magnetic DNA Purification System 200 preps FF3750 1336.00<br />

for Food<br />

Available Separately<br />

400 preps FF3751 2249.00<br />

Lysis Buffer A, Food 100 ml A8191 117.00<br />

Lysis Buffer B, Food 100 ml Z3191 345.00<br />

Precipitation Solution, Food 150 ml Z3201 705.00<br />

A8191, Z3191, Z3201 For Research Use Only. Not for Use in Diagnostic Procedures.<br />

Description: The Wizard ® Magnetic DNA Purification System for Food is<br />

designed for purification of DNA from a variety of food samples including corn<br />

seeds, cornmeal, soybeans, soy flour and soy milk. Processed food, such as<br />

corn chips, chocolate and chocolate-containing foods, lecithin and vegetable<br />

oils may also be used with the suggested protocol variations. The DNA purified<br />

from these samples can be used in PCR-based testing for genetically modified<br />

organism (GMO) DNA sequences.<br />

Features:<br />

• Improved Productivity: Obtain results in one-third the time of current<br />

methods.<br />

• Ease of Handling: Requires minimal centrifugation and eliminates organic<br />

extractions.<br />

• Versatility and Robustness: Validated with a broad variety of foodstuffs,<br />

including difficult samples such as lecithin and vegetable oils.<br />

Storage Conditions: Store at 22–25°C.<br />

Protocol Part#<br />

Wizard ® Magnetic DNA Purification System for Food Technical<br />

Bulletin<br />

TB284<br />

115<br />

5<br />

DNA and RNA Purification<br />

Section<br />

Contents<br />

Table of<br />

Contents

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