2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
114
MagneSil ® Genomic, Large Volume System
Product Size Cat.# Price ($)
MagneSil ® Genomic, Large Volume System 8 preps A4080 185.00
48 preps A4082 1098.00
96 preps A4085 2192.00
Available Separately
eLysis Buffer, Large Volume System
For Laboratory Use.
1 L A4091 371.00
Description: The MagneSil ® Genomic, Large Volume System, is designed
for scalable, automated genomic DNA isolation from large-volume samples,
eliminating laborious centrifugation steps and the use of hazardous organic
solvents. The system has been automated on the Tecan Freedom EVO ® liquid
handler, providing walkaway purification of genomic DNA from a variety of
starting materials, including 1–10ml whole blood samples, regardless of
sample storage or shipping conditions. The instrument uses only the amount
of reagents required to process each sample, maximizing efficiency and value
per prep.
The MagneSil ® Genomic, Large Volume System, uses a robust
noncentrifugation-based automated method to purify genomic DNA from
fresh, frozen or mishandled blood and other samples with similar yields and
quality. The system bypasses many of the challenges of traditional centrifugation-based
methods by lysing the entire whole blood sample and then
directly capturing total genomic DNA from the lysed sample using MagneSil ®
Paramagnetic Particles (PMPs). The genomic DNA bound to the MagneSil ®
PMPs is washed to remove contaminants such as heme and cellular proteins,
then eluted into an aqueous solution ready for use in downstream applications.
There is no need for tedious and lengthy DNA rehydration. The purified genomic
DNA is suitable for a variety of downstream applications such as single and
multiplex PCR, restriction digestion and real-time PCR.
Features:
• Improve Productivity: Walkaway automation from blood-collection tube
to application-ready DNA.
• Rely on an Integrated Solution: One reagent system and automated
method provide yield and purity from any sample type (fresh or frozen
blood, samples of unknown quality and mixed sample populations).
• Enjoy Smart Scalability: Scale sample size from 1–10ml of blood, batch
size from 1–96 samples and reagent usage from input sample volume.
• Achieve Turnkey Automation: Optimized protocol available for the Tecan
Freedom EVO ® instrument. This and other validated automated methods
are available at: www.promega.com/automethods/
Storage Conditions: Store at 22–25°C.
Protocol Part#
MagneSil ® Genomic, Large Volume System Technical Bulletin TB549
Fixed-Tissue Genomic DNA Purification
Product Size Cat.# Price ($)
MagneSil ® Genomic, Fixed Tissue System 100 samples MD1490 493.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The MagneSil ® Genomic, Fixed Tissue System provides a fast,
simple technique to prepare genomic DNA from formalin-fixed,
paraffin-embedded tissue. After an overnight Proteinase K digestion, genomic
DNA can be manually purified from formalin-fixed, paraffin-embedded thin
tissue sections in less than an hour. Amplifiable genomic DNA can be isolated
from 10μm thin sections without centrifugation of the lysate prior to
purification. Up to 12 samples can be processed in the manual format using
the MagneSphere ® Technology Magnetic Separation Stand (twelve-position)
(Cat.# Z5342).
Features:
• Purify High-Quality DNA: The composition of the wash buffers and
protocol have been optimized to yield genomic DNA that is largely free of
small DNAs, a potent inhibitor of PCR amplification.
• Rely on Performance-Tested Amplification Results: Amplify targets in
multiplex PCR and targets as large as 450–1,800bp.
Storage Conditions: MD1490 consists of two separate items shipped at
different temperatures. MD1170 (part 1 of 2 for MD1490 - Processing Module)
contains Proteinase K, DTT and Incubation Buffer, which are shipped on dry ice.
Store MD1170 at –20°C.
MD1180 (part 2 of 2 for MD1490 - Purification Module) contains Lysis Buffer,
2X Wash Buffer, Resin and Elution Buffer, which are shipped at room
temperature, 22–25°C. Store MD1180 at room temperature, 22–25°C.
Protocol Part#
MagneSil ® Genomic, Fixed Tissue System Technical Bulletin TB319
ReadyAmp Genomic DNA Purification
System
Product
ReadyAmp Genomic DNA Purification
Size Cat.# Price ($)
System 100 reactions A7710 313.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The ReadyAmp Genomic DNA Purification System yields
single-stranded DNA (ssDNA) from whole blood or blood stains that may be
used directly in amplification reactions without further manipulation. The
process takes less than one hour and requires no organic extractions or ethanol
precipitations.
Features:
• Simple and Effective: ReadyAmp resin removes PCR inhibitors.
• Convenient: Isolated DNA can be used directly in PCR amplifications.
Storage Conditions: Store at 22–25°C.
Protocol Part#
ReadyAmp Genomic DNA Purification System Technical Bulletin TB190
For complete and up-to-date product information visit: www.promega.com/catalog