2012 Promega catalogue

Cell Signaling
MagneSil ® ONE, Fixed Yield Blood Genomic
System
Product Size Cat.# Price ($)
MagneSil ® ONE, Fixed Yield Blood Genomic
System 1 × 96 preps MD1370 454.00
Collection Plates (4-pack) 1 each A9161 24.00
Available Separately
Lysis Buffer, Blood 160 ml MD1392 158.00
Anti-Foam Reagent 300 µl MD1431 48.00
MagneSil ® PMPs—Fixed Yield 25 ml MD1451 368.00
Alcohol Wash, Blood 120 ml MD1412 91.00
Elution Buffer, Blood 45 ml MD1421 48.00
MD1370, MD1431, MD1451, MD1412 For Laboratory Use. MD1392, A9161, MD1421 For
Research Use Only. Not for Use in Diagnostic Procedures.
Description: The MagneSil ® ONE, Fixed Yield Blood Genomic System purifies
1μg of DNA (+/– 50%) from 60μl of anti-coagulated whole blood.
Purification of a “fixed yield” of DNA eliminates the need to quantitate and
normalize concentrations postpurification. The highly pure DNA isolated is
suitable for use in PCR, multiplex PCR and SNP genotyping applications.
Walkaway automation is available on the Beckman Coulter Biomek ® FX in a
96-well format. Process 96 samples in about 1 hour with no hands-on time
following robot setup.
Features:
• Improve Productivity: Use walkaway automation to extract genomic DNA
and eliminate DNA quantitation prior to PCR.
• Achieve Consistent Results: Obtain 1μg (fixed yield) of highly pure DNA
from 60μl of blood.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at 20–25°C.
Protocol Part#
MagneSil ® ONE, Fixed Yield Blood Genomic System Technical
Bulletin
TB313
For complete and up-to-date product information visit: www.promega.com/catalog
MagneSil ® Blood Genomic, Max Yield System
Product
MagneSil
Size Cat.# Price ($)
® Blood Genomic, Max Yield
System
Available Separately
1 × 96 preps MD1360 393.00
Anti-Foam Reagent 300 µl MD1431 48.00
MagneSil ® Paramagnetic Particles 25 ml MD1441 Pls. Enq.
Salt Wash, Blood 90 ml MD1401 158.00
Alcohol Wash, Blood 70 ml MD1411 58.00
Elution Buffer, Blood 45 ml MD1421 48.00
MD1431, MD1441 For Laboratory Use. MD1360, MD1401, MD1411, MD1421 For Research Use
Only. Not for Use in Diagnostic Procedures.
Description: The MagneSil ® Blood Genomic, Max Yield System provides
automated high-throughput DNA purification on the Beckman Coulter Biomek ®
FX using MagneSil ® Paramagnetic Particle technology. DNA from 96 samples
of anti-coagulated human whole blood is purified in about 1 1/2 hours with no
hands-on time once the robot protocol is initiated. Studies on DNA recovery
and purity and PCR results show no cross-contamination between samples in
adjacent wells. Purified DNA is qualified for single-locus “simple PCR” as well
as more demanding applications such as multiplex PCR (e.g., PowerPlex ® 16
System [Cat.# DC6531], Y Chromosome Deletion Detection System [Cat.#
MD1531]) and SNP genotyping.
Features:
• Improve Productivity: Walkaway automation of genomic DNA extraction.
• Achieve Maximum Yield: The average yield of 96 purified samples from
normal healthy adults is ≥4μg.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at 22–25°C.
Protocol Part#
MagneSil ® Blood Genomic, Max Yield System Technical Bulletin TB312
113
5
DNA and RNA Purification
Section
Contents
Table of
Contents