2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
112
Wizard ® SV Genomic DNA Purification System
Product Size Cat.# Price ($)
Wizard ® SV Genomic DNA Purification System 50 preps A2360 160.00
250 preps A2361 716.00
Available Separately
Wizard ® SV Lysis Buffer 50 ml Z3052 66.00
Wizard ® SV Wash Solution 185 ml A1311 156.00
Nuclei Lysis Solution 50 ml A7941 51.00
EDTA, 0.5M (pH 8.0), Molecular Biology Grade 100 ml V4231 32.00
RNase A Solution, 4mg/ml 1 ml A7973 415.00
Z3052, A1311 For Laboratory Use. A2360, A2361, A6770, A7941, A6772, V4231, A6774, A7973
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Wizard ® SV Genomic DNA Purification System provides a
fast, simple, membrane-based technique for preparing genomic DNA from
cultured cells and tissue, including mouse tails. Genomic DNA can be purified
from cultured cells in about 20 minutes. Isolation from tissue or mouse tails
requires an overnight digestion with Proteinase K (Cat.# V3021). Amplifiable
genomic DNA can be isolated from up to 5 × 10 6 cells, 20mg of tissue or up to
1.2cm of a mouse tail tip without a centrifugation clearing step.
The Wizard ® SV Genomic DNA Purification System can be used in either a
microcentrifuge (spin) or vacuum protocol. Up to 20 samples can be processed
at once in the vacuum format with the Vac-Man ® Laboratory Vacuum Manifold
(Cat.# A7231) and the Vacuum Adapters (Cat.# A1331).
Features:
• Improved Productivity: Obtain genomic DNA approximately 20 minutes
after lysis.
• High Yield: Purify 20–30μg of DNA per prep from 1.2cm mouse tail.
• Format Choice: Perform purification by either spin or vacuum formats.
Storage Conditions: Store at 22–25°C.
Protocol Part#
Wizard ® SV Genomic DNA Purification System Technical Bulletin TB302
Average Yield of Genomic DNA Purifi ed From Various Tissues Using
the Wizard ® SV and SV 96 Genomic DNA Purifi cation Systems.
Sample Type Starting Amount Average Yield
Mouse Tail Clipping 20mg 20µg
Mouse Liver 20mg 15µg
Mouse Heart 20mg 10µg
Mouse Brain 20mg 6µg
CHO Cells 1 × 10 6 cells 5µg
NIH/3T3 Cells 1 × 10 6 cells 9µg
293 Cells 1 × 10 6 cells 8µg 9484LA
Wizard ® SV 96 Genomic DNA Purification
System
Product Size Cat.# Price ($)
Wizard ® SV 96 Genomic DNA Purification 1 × 96 preps A2370 429.00
System
Available Separately
4 × 96 preps A2371 1568.00
Wizard ® SV Lysis Buffer 50 ml Z3052 66.00
Wizard ® SV Wash Solution 185 ml A1311 156.00
Nuclei Lysis Solution 50 ml A7941 51.00
EDTA, 0.5M (pH 8.0), Molecular Biology Grade 100 ml V4231 32.00
RNase A Solution, 4mg/ml 1 ml A7973 415.00
Wizard ® SV 96 Binding Plates 10 pack A2271 1450.00
Z3052, A1311 For Laboratory Use. A2370, A2371, A6780, A7941, A6782, V4231, A6784,
A7973, A2271 For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Wizard ® SV 96 Genomic DNA Purification System provides
a high-throughput, membrane-based technique for consistent preparation of
genomic DNA from cultured cells and tissue, including mouse tails. Amplifiable
genomic DNA can be isolated from up to 5 × 10 6 cells, 20mg of tissue or up to
1.2cm of a mouse tail tip without a centrifugation clearing step.
With the Wizard ® SV Genomic DNA purification system, genomic DNA is
purified from cell lysates using 96-well vacuum filtration. Washing the bound
DNA requires no disassembly of the manifold, and filtrate waste products
are delivered directly to a vacuum trap, eliminating the need to empty waste
collection trays.
The Wizard ® SV Genomic DNA Purification System is designed for use either
in a manual format or with Beckman Coulter or PerkinElmer automated instruments.
Features:
• Improve Productivity: Obtain genomic DNA from mouse tails in
45–60 minutes, genomic DNA from cultured cells in 30 minutes. No
centrifugation required.
• Achieve High Yield: Purify 20–30μg of DNA per prep from 1.2cm of
mouse tail.
• Gain Confidence in Applications: Purified DNA ready for amplification.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at 22–25°C.
Protocol Part#
Wizard ® SV 96 Genomic DNA Purification System Technical Bulletin TB303
For complete and up-to-date product information visit: www.promega.com/catalog