2012 Promega catalogue

Cell Signaling
ReliaPrep FFPE gDNA Miniprep System
Product Size Cat.# Price ($)
ReliaPrep FFPE gDNA Miniprep System 10 reactions A2351 150.00
For Research Use Only. Not for Use in Diagnostic Procedures.
100 reactions A2352 830.00
Description: The ReliaPrep FFPE gDNA Miniprep System provides a
complete, all-inclusive method for purifying quality genomic DNA from
formalin-fixed paraffin-embedded tissue without using hazardous solvents or
overnight digestion. Genomic DNA can be isolated from FFPE tissue in
approximately 2 1/2 hours with minimal hands-on time.
Features:
• Isolate Quality, Intact gDNA: Optimized lysis and binding conditions
reverse modifications introduced by the fixation process, resulting in intact,
amplifiable gDNA.
• Safely Deparaffinize Your Sample: Deparaffinization step occurs
without harsh organic solvents.
• Save Time: Purify gDNA from FFPE tissue in less than 2 1/2 hours with
minimal hands-on time. No overnight digestion required.
• Easy to Use: Minimal preparation time; simply add ethanol and go!
Storage Conditions: Store at room temperature.
Protocol Part#
ReliaPrep FFPE gDNA Miniprep System Technical Manual TM352
For complete and up-to-date product information visit: www.promega.com/catalog
Wizard ® Genomic DNA Purification Kit
Product Size Cat.# Price ($)
Wizard ® Genomic DNA
100 isolations × 300 µl A1120 282.00
Purification Kit
500 isolations × 300 µl A1125 788.00
Available Separately
100 isolations × 10 ml A1620 1074.00
Cell Lysis Solution (Genomic Purification) 1 liter A7933 203.00
Nuclei Lysis Solution 50 ml A7941 51.00
1 liter A7943 522.00
Protein Precipitation Solution 25 ml A7951 68.00
350 ml A7953 462.00
DNA Rehydration Solution 50 ml A7963 73.00
RNase A Solution, 4mg/ml 1 ml A7973 415.00
Proteinase K 100 mg V3021 156.00
A7933 For Laboratory Use. A1120, A1125, A7941, A1620, A7943, A7951, A7953, A7963,
A7973, V3021 For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Wizard ® Genomic DNA Purification Kit provides a simple,
solution-based method for isolation of DNA from white blood cells, tissue
culture cells, animal tissue, plant tissue, yeast and Gram-positive and
Gram-negative bacteria. DNA purified with this system is suitable for a variety
of applications, including amplification, digestion with restriction endonucleases
and membrane hybridizations (e.g., Southern and dot/slot blots).
Features:
• Improved Productivity: Rapidly isolate genomic DNA from blood, tissue
culture, animal and plant cells, bacteria and yeast in approximately
60 minutes.
• Scalability: Reagent volumes can be adjusted to correspond to the
amount of material to be processed.
• Flexibility: Genomic DNA purified from a variety of sample types is
suitable for a variety of applications.
• Your Choice of Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at 22–25°C.
Protocol Part#
Wizard ® Genomic DNA Purification Kit Technical Manual TM050
DNA Yields from Various Starting Materials Using the Wizard ®
Genomic DNA Purifi cation Kit.
Amount of Typical
Source Starting Material DNA Yield
Whole Blood 300µl 5–15µg
1ml 25–50µg
10ml 250–500µg
96-well plate, 50µl/well 0.2–0.7µg
Tissue Culture Cells 10 6 –10 7 cells 5–30µg
Animal Tissue
Mouse Liver 11mg 15–20µg
Mouse Tail 0.5–1cm of tail 10–30µg
Insect Cells 5 × 10 6 cells 16µg
Plant Leaf Tissue 40mg 7–12µg
Bacterial Culture* 10 8 –10 10 cells 5–20µg
Yeast* 1.9 × 10 8 cells 4.5–6.5µg
*Overnight culture. 9483LA
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5
DNA and RNA Purification
Section
Contents
Table of
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