2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
Genomic DNA Purification
110
ReliaPrep Large Volume HT gDNA Isolation
System
Product Size Cat.# Price ($)
ReliaPrep Large Volume HT gDNA
Isolation System
Available Separately
96 × 10ml preps A1751 Pls. Enq.
ReliaPrep LV 32 HSM Instrument
ReliaPrep LV 32 HSM Standard Service
1 each A1715 Pls. Enq.
Agreement 1 each SA3070 Pls. Enq.
ReliaPrep Cover 1 each A1712 Pls. Enq.
ReliaPrep Tube Rack 1 each A1713 Pls. Enq.
ReliaPrep Tube Rack Stand 1 each A1714 Pls. Enq.
A1751 For Laboratory Use. A1715, SA3070, A1712, A1713, A1714 For Research Use Only. Not
for Use in Diagnostic Procedures. Product may not be available in all countries. Please contact
your local representative for more information.
Description: The ReliaPrep Large Volume HT gDNA Isolation System
isolates genomic DNA (gDNA) from blood samples ranging from 3ml to 10ml
of blood in a scalable format. The chemistry eliminates tedious
centrifugation steps as well as the use of hazardous chemicals, which are
inherent in precipitation-based chemistries. The system has been automated
on robotic liquid-handling workstations, allowing walkaway purification of
genomic DNA from 3–10ml of whole blood, regardless of sample storage or
shipping conditions. For low-throughput isolation of gDNA from up to
32 samples at one time, the ReliaPrep LV 32 HSM can be used in a manual
mode, where the user performs the pipetting functions. The HSM has an LCD
screen that directs the user through the protocol.
Features:
• Decrease Hands-On Time: Automation reduces operator time spent on
instrument setup and takedown by allowing walkaway operation for large
numbers of samples at a time.
• Remove Protocol Bottlenecks: Heater Shaker Magnet eliminates the
need to move samples on the robot deck, reducing instrument failures;
precipitation-free chemistry dramatically reduces purification failures.
• Achieve Peace of Mind: Automated liquid level sensing for all samples
and solutions with operator notification allows recovery of samples in case
of error.
• Isolate Pure DNA from All Samples: Purification chemistry is equally
effective at recovering DNA from pristine as well as challenged (hemolysed
or frozen) samples.
• Save a Day or Two of Processing: Samples are eluted in buffer, ready
for use in downstream assays or archiving, eliminating resuspension of
pelleted DNA, which can take 24–48 hours.
• Reduce Waste: Chemistry is automatically scaled for each sample,
using only the reagent required for optimal purification. Plastic use is also
conserved, reducing liquid and solid waste during sample runs.
Storage Conditions: Store at 15–30°C.
Protocol Part#
ReliaPrep Large Volume HT gDNA Isolation System Technical
Manual
TM341
ReliaPrep Blood gDNA Miniprep System
Product Size Cat.# Price ($)
ReliaPrep Blood gDNA Miniprep System 100 preps A5081 495.00
For Laboratory Use.
250 preps A5082 1090.00
Description: The ReliaPrep Blood gDNA Miniprep System provides a
complete, ready-to-use method for purification of gDNA from up to 200μl of
blood or body fluid, consistently isolating pure, intact gDNA without the use of
alcohol washes or precipitations. Genomic DNA can be prepared from fresh
or frozen blood in less than 40 minutes with expected DNA yields of 4–10μg,
depending on the white blood cell count of the blood sample.
Features:
• Easy to Use: Reagents are supplied “ready to go”; no additions required.
• Save Time: Eluted DNA obtained in 30 minutes or less.
• No Ethanol: Eliminates alcohol inhibition and carryover.
• Pure gDNA: Improved A 260/A 230 ratios vs. the leading competitor.
• Peace of Mind: Consistent results from run to run and between users
even with hemolyzed samples.
• Concentrated DNA: Good recovery and purity in as little as 50μl elution.
Storage Conditions: Store at 15–30°C.
Protocol Part#
ReliaPrep Blood gDNA Miniprep System Technical Manual TM330
ReliaPrep gDNA Tissue Miniprep System
Product Size Cat.# Price ($)
ReliaPrep gDNA Tissue Miniprep System 100 preps A2051 495.00
For Laboratory Use.
250 preps A2052 1090.00
Description: The ReliaPrep gDNA Tissue Miniprep System provides a complete,
ready-to-use method for purification of gDNA from up to 25mg of tissue,
a buccal (cheek) swab, or a 1cm mouse tail snip, obtaining intact gDNA without
the use of ethanol washes or precipitations.
Features:
• Easy to Use: Reagents are supplied “ready-to-use”; no additions required.
• Save Time: Eluted DNA obtained in 30 minutes or less (hands-on time).
• No Ethanol: Eliminates alcohol inhibition and carryover.
• Pure gDNA: Improved A 260/A 230 ratios vs. the leading competitor.
• Peace of Mind: Consistent results from run to run and between users.
• Concentrated DNA: Good recovery and purity in as little as 50μl elution.
Storage Conditions: Store at 15–30°C.
Protocol Part#
ReliaPrep gDNA Tissue Miniprep System Technical Manual TM345
For complete and up-to-date product information visit: www.promega.com/catalog