2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 108 Wizard ® SV 96 PCR Clean-Up System Product Size Cat.# Price ($) Wizard ® SV 96 PCR Clean-Up System 1 × 96 preps A9340 276.00 4 × 96 preps A9341 1039.00 8 × 96 preps A9342 1929.00 100 × 96 preps A9345 Pls. Enq. Available Separately Membrane Binding Solution 20 ml A9301 81.00 Wizard ® SV 96 Binding Plates For Research Use Only. Not for Use in Diagnostic Procedures. 10 pack A2271 1450.00 Description: The Wizard ® SV 96 PCR Clean-Up System is designed for highthroughput purification of 100bp to 10kb PCR products from excess nucleotides, primers and primer dimers. This membrane-based system recovers >90% PCR fragments in as little as 20 minutes. The purified DNA can be used for automated fluorescent sequencing, cloning, labeling, restriction digestion or microarray analysis without further manipulation. The Wizard ® SV 96 PCR Clean-Up System uses 96-well filtration without the need to disassemble the manifold. Filtrate waste is delivered directly to a vacuum trap, eliminating the need to dispose of collected waste within the manifold assembly. Protocols are available for automated instruments from Beckman Coulter and PerkinElmer. Features: • High Performance: Optimized methods deliver purified PCR products suitable for demanding applications such as microarray analysis. • Confidence: Average recovery for 100–500bp fragments of >90%. Automated fluorescent sequencing Phred* 20 scores >600. • Automation: Validated automated methods available at: www.promega.com/automethods/ • Your Choice of Configuration: Learn more about our custom options for this product at: www.promega.com/myway/ *A Phred score is a widely recognized method to measure the quality of DNA sequences. Phred is a base-calling program for DNA sequence traces available from Codoncode Corporation. Storage Conditions: Store at 22–25°C. Protocol Part# Wizard ® SV 96 PCR Clean-Up System Technical Bulletin TB311 A. + Betaine – Betaine U P P P P U P P P P B. 1. 2. Microarray of purified PCR products. PCR products (300bp) were amplified in the presence or absence of 1M betaine, then purified using the Wizard ® SV 96 PCR Clean-Up System. Panel A. Agarose gel analysis. Purified (P) and unpurified (U) PCR products amplified with (+) or without (–) betaine were separated on an ethidium bromide-stained, 2% agarose gel. Panel B. Representative microarray blocks of purified PCR product hybridized to complementary Cy ® -labeled cDNA. Betaine interferes with microarray analysis, so the fact that the microarray data for PCR with and without betaine are equivalent clearly demonstrates removal of betaine using the Wizard ® SV 96 PCR Clean-Up System. 1. PCR product amplified under standard amplification conditions (–betaine). 2. 1M betaine added to the PCR mix. For complete and up-to-date product information visit: www.promega.com/catalog 3796TA08_2A
Cell Signaling Wizard ® MagneSil ® Sequencing Reaction Clean-Up System Product Size Cat.# Price ($) Wizard ® MagneSil ® Sequencing Reaction Clean-Up System 4 × 96 preps A1831 504.00 8 × 96 preps A1832 951.00 Wizard ® MagneSil ® Sequencing Reaction Clean-Up System, HTP1 100 × 96 preps A1835 Pls. Enq. Available Separately MagneSil ® GREEN For Research Use Only. Not for Use in Diagnostic Procedures. 100 ml A8231 504.00 Description: The Wizard ® MagneSil ® Sequencing Reaction Clean-Up System was developed for high-throughput purification of sequencing reactions, including BigDye ® Terminator reactions. Cleanup is performed using the proprietary MagneSil ® GREEN Paramagnetic Particles with standard, nonskirted 96-well amplification plates. No user intervention is required from the time the plates are placed on the instrument until the samples are ready for loading onto the fluorescent DNA sequencer. Protocols are available for automated instruments from Beckman Coulter and Tecan. The system relies upon the MagnaBot ® II for magnetic separation. The Plate Clamp 96 and Plate Stand are recommended for automated use because they ensure PCR plates are uniformly flat for liquid transfer on a robotic instrument. Features: • Get Immediate Results: Validated, walkaway method. • Gain Confidence in Results: Purified products are approved for fluorescent sequencing reactions. Phred* 20 quality scores ≥650 bases. • Automate This Assay: Validated automated methods available at: www.promega.com/automethods/ *A Phred score is a widely recognized method to measure the quality of DNA sequences. Phred is a base-calling program for DNA sequence traces available from Codoncode Corporation. Storage Conditions: Store at 22–25°C. Protocol Part# TB287 Wizard ® MagneSil ® Sequencing Reaction Clean-Up System Technical Bulletin For complete and up-to-date product information visit: www.promega.com/catalog Electropherogram of purified sequencing reactions analyzed on an ABI PRISM ® 3700 DNA Sequencer. BigDye ® terminator reactions purified with the Wizard ® MagneSil ® Sequencing Reaction Clean-Up System. Cleanup is performed using the MagnaBot ® II Magnetic Separation Device (Cat.# V8351) accompanied by the Plate Clamp 96 (Cat.# V8251). These devices are designed to work with most robotic platforms. 3250CC03_1A 3312TA03_1A 109 5 DNA and RNA Purification Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
Page 61 and 62: Cell Signaling Kinase Enzyme System
Page 63 and 64: Cell Signaling Kinase Enzyme System
Page 65 and 66: Cell Signaling Kinase-Glo ® Kinase
Page 67 and 68: Cell Signaling SignaTECT ® Protein
Page 69 and 70: Cell Signaling Casein Kinase I Prod
Page 71 and 72: Cell Signaling Product Size Cat.# P
Page 73 and 74: Cell Signaling Protein Kinase Subst
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Page 77 and 78: Cell Signaling DNA Step Ladders Pro
Page 79 and 80: Cell Signaling Conventional DNA Mar
Page 81 and 82: Cell Signaling ProMega-Markers ® L
Page 83 and 84: Cell Signaling Restriction Enzymes
Page 85 and 86: Cell Signaling BamHI Product Size C
Page 87 and 88: Cell Signaling EcoRI Product Size C
Page 89 and 90: Cell Signaling NciI Product Size Co
Page 91 and 92: Cell Signaling SpeI Product Size Co
Page 93 and 94: Cell Signaling TSAP Thermosensitive
Page 95 and 96: Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98: Cell Signaling Kinases T4 Polynucle
Page 99 and 100: Cell Signaling S1 Nuclease Product
Page 101 and 102: Cell Signaling Percent Inhibition o
Page 103 and 104: Cell Signaling Untagged Flexi ® Ma
Page 105 and 106: Cell Signaling pGEM ® -5Zf(+) Vect
Page 107 and 108: Cell Signaling pSP64 Poly(A) Vector
Page 109 and 110: Cell Signaling For complete and up-
Page 111: Cell Signaling x-tracta Gel Extract
Page 115 and 116: Cell Signaling ReliaPrep FFPE gDNA
Page 117 and 118: Cell Signaling MagneSil ® ONE, Fix
Page 119 and 120: Cell Signaling MagneSil ® KF, Geno
Page 121 and 122: Cell Signaling Plasmid Purification
Page 123 and 124: Cell Signaling PureYield Plasmid Ma
Page 125 and 126: Cell Signaling Wizard ® Plus Maxip
Page 127 and 128: Cell Signaling Wizard ® MagneSil
Page 129 and 130: Cell Signaling PureYield RNA Midipr
Page 131 and 132: Cell Signaling Maxwell ® 16 System
Page 133 and 134: Cell Signaling RNasin ® Plus RNase
Page 135 and 136: Cell Signaling DNA Quantitation Qua
Page 137 and 138: Cell Signaling Magnetic Stands and
Page 139 and 140: Cell Signaling 6 Drug Discovery For
Page 141 and 142: Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144: Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146: Cell Signaling Protease Assays Prot
Page 147 and 148: Cell Signaling Cell-Based Proteasom
Page 149 and 150: Cell Signaling Tryptase, Human, Rec
Page 151 and 152: Cell Signaling 7 Genetic Identity F
Page 153 and 154: Cell Signaling Maxwell ® 16 Forens
Page 155 and 156: Cell Signaling Plexor ® HY System
Page 157 and 158: Cell Signaling PowerPlex ® 18D Sys
Page 159 and 160: Cell Signaling PowerPlex ® CS7 Sys
Page 161 and 162: Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Microbial Detection
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10 Instruments For complete and up-
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling Maxwell ® 16 System
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling dNTP Mix Product Siz
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Cell Signaling 12 PCR For complete
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Cell Signaling TaqBead Hot Start Po
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Cell Signaling GoTaq ® PCR Core Sy
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling Plexor ® qPCR and q
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Cell Signaling StemElite Differenti
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
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Cell Signaling HaloCHIP System Prod
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Cell Signaling MagneHis Protein Pur
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Cell Signaling Streptavidin Product
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Cell Signaling Protein Purification
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Cell Signaling 15 Reporter Assays a
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Cell Signaling Dual-Glo ® Lucifera
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Cell Signaling Firefly Luciferase R
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Cell Signaling Bright-Glo Luciferas
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Cell Signaling EnduRen Live Cell Su
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Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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2012 Promega catalogue

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