2012 Promega catalogue

Life
Science
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2012
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Cell Signaling
108
Wizard ® SV 96 PCR Clean-Up System
Product Size Cat.# Price ($)
Wizard ® SV 96 PCR Clean-Up System 1 × 96 preps A9340 276.00
4 × 96 preps A9341 1039.00
8 × 96 preps A9342 1929.00
100 × 96 preps A9345 Pls. Enq.
Available Separately
Membrane Binding Solution 20 ml A9301 81.00
Wizard ® SV 96 Binding Plates
For Research Use Only. Not for Use in Diagnostic Procedures.
10 pack A2271 1450.00
Description: The Wizard ® SV 96 PCR Clean-Up System is designed for highthroughput
purification of 100bp to 10kb PCR products from excess nucleotides,
primers and primer dimers. This membrane-based system recovers
>90% PCR fragments in as little as 20 minutes. The purified DNA can be used
for automated fluorescent sequencing, cloning, labeling, restriction digestion
or microarray analysis without further manipulation. The Wizard ® SV 96 PCR
Clean-Up System uses 96-well filtration without the need to disassemble the
manifold. Filtrate waste is delivered directly to a vacuum trap, eliminating the
need to dispose of collected waste within the manifold assembly. Protocols are
available for automated instruments from Beckman Coulter and PerkinElmer.
Features:
• High Performance: Optimized methods deliver purified PCR products
suitable for demanding applications such as microarray analysis.
• Confidence: Average recovery for 100–500bp fragments of >90%.
Automated fluorescent sequencing Phred* 20 scores >600.
• Automation: Validated automated methods available at:
www.promega.com/automethods/
• Your Choice of Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
*A Phred score is a widely recognized method to measure the quality of DNA
sequences. Phred is a base-calling program for DNA sequence traces available
from Codoncode Corporation.
Storage Conditions: Store at 22–25°C.
Protocol Part#
Wizard ® SV 96 PCR Clean-Up System Technical Bulletin TB311
A.
+ Betaine – Betaine
U P P P P U P P P P
B.
1. 2.
Microarray of purified PCR products. PCR products (300bp) were
amplified in the presence or absence of 1M betaine, then purified using the
Wizard ® SV 96 PCR Clean-Up System. Panel A. Agarose gel analysis. Purified
(P) and unpurified (U) PCR products amplified with (+) or without (–) betaine
were separated on an ethidium bromide-stained, 2% agarose gel.
Panel B. Representative microarray blocks of purified PCR product hybridized
to complementary Cy ® -labeled cDNA. Betaine interferes with microarray
analysis, so the fact that the microarray data for PCR with and without betaine
are equivalent clearly demonstrates removal of betaine using the Wizard ®
SV 96 PCR Clean-Up System. 1. PCR product amplified under standard
amplification conditions (–betaine). 2. 1M betaine added to the PCR mix.
For complete and up-to-date product information visit: www.promega.com/catalog
3796TA08_2A