2012 Promega catalogue

Cell Signaling
x-tracta Gel Extractor
Product Size Cat.# Price ($)
x-tracta Gel Extractor 25 /pack A2121 26.00
100 /pack A2122 98.00
Wizard ® SV Gel and PCR Clean-
Up System and x-tracta Gel
50 preps/25 extractors A9283 123.00
Extractor Bundle
250 preps/100 extractors A9284 534.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The x-tracta Gel Extractor tool provides a convenient, safe
method for removing agarose gel fragments for further processing. The device
removes a 0.13 × 0.33 inch gel piece from agarose gels for easy transfer into
a microcentrifuge tube for processing. The x-tracta tool eliminates the need
for razor blades or scalpels, and its single-use design eliminates the possibility
for sample-to-sample cross-contamination.
Note: The x-tracta Gel Extractor works best on 0.6–2% analytical grade
agarose gels. Please exercise caution if using the x-tracta Gel Extractor on
Low Melting Point (LMP) agarose gels because the extractor does not work
effectively on these due to the gel consistency.
Storage Conditions: Store at 22–25°C.
Protocol Part#
x-tracta Gel Extractor Tool GE449
Wizard ® PCR Preps DNA Purification System
Product Size Cat.# Price ($)
Wizard ® PCR Preps DNA Purification System 50 preps A7170 230.00
Available Separately
250 preps A2180 758.00
Wizard ® PCR Preps DNA Purification Resin 250 ml A7181 351.00
Direct Purification Buffer 25 ml A7241 83.00
Wizard ® Minicolumns 250 each A7211 351.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Wizard ® PCR Preps DNA Purification System provides a
simple, reliable way to purify double-stranded PCR-amplified DNA. Using the
15-minute batch column purification method, PCR products are effectively
separated from contaminants, including primer-dimers and amplification primers.
This system also can be used to purify DNA fragments from agarose gels.
The DNA can be eluted in water or TE buffer, free of salts or macromolecular
contaminants. Multiple PCR Preps may be processed easily at one time with
the Vac-Man ® Laboratory Vacuum Manifold (Cat.# A7231).
Features:
• Improved Productivity: Purify PCR products directly from reactions in 15
minutes.
• Flexibility: Separate PCR products from other reaction components such
as primers and primer-dimers or from gel slices.
• Labor Saving Format: Process multiple purifications simultaneously
using the Vac-Man ® Laboratory Vacuum Manifold.
Storage Conditions: Store at 22–25°C.
Protocol Part#
Wizard ® PCR Preps DNA Purification System Technical Bulletin TB118
1,000 –
500 –
50 –––––
200 –
For complete and up-to-date product information visit: www.promega.com/catalog
bp
Before Purification After Purification
M M
Recovery of PCR products using Wizard ® PCR Preps Resin. A
representative sample from the simultaneous purification of 96 PCR
products was chosen to determine the effectiveness of the procedure by gel
electrophoresis. Equivalent amounts from before and after purification were
separated on a 1% agarose gel and stained with ethidium bromide.
Wizard ® DNA Clean-Up System
Product Size Cat.# Price ($)
Wizard ® DNA Clean-Up System 100 preps A7280 336.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Wizard ® DNA Clean-Up System provides a simple and
effective way to purify linear and circular DNA (200–50,000bp) from many
molecular biology reactions. Using a quick batch-column procedure, the entire
process can be completed in 15 minutes or less with no organic extractions or
ethanol precipitations. DNA is eluted in water or TE buffer, ready for use.
Features:
• Improved Productivity: Results in 15 minutes or less.
• Convenience: No phenol extractions or ethanol precipitations.
• Flexibility: Works with a wide range of DNA sizes from 200–50,000bp in
length.
Storage Conditions: Store at 22–25°C.
Protocol Part#
Wizard ® DNA Clean-Up System Technical Bulletin TB141
0935TA11_4A
107
5
DNA and RNA Purification
Section
Contents
Table of
Contents