2012 Promega catalogue

Life
Science
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2012
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Cell Signaling
DNA Fragment Purification
106
Wizard ® SV Gel and PCR Clean-Up System
Product Size Cat.# Price ($)
Wizard ® SV Gel and PCR
Clean-Up System
50 preps A9281 100.00
250 preps A9282 448.00
1,000 preps A9285 1600.00
Wizard ® SV Gel and PCR Clean-
Up System and x-tracta Gel
50 preps/25 extractors A9283 123.00
Extractor Bundle
Available Separately
250 preps/100 extractors A9284 534.00
Membrane Binding Solution 20 ml A9301 81.00
Vacuum Adapters 20 each A1331 33.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Wizard ® SV Gel and PCR Clean-Up System is designed to
extract and purify DNA fragments of 100bp to 10kb from standard or
low-melting agarose gels or to purify products directly from PCR and other
common reactions such as restriction digests. Up to 95% recovery is achieved
depending upon the DNA fragment size. PCR products are commonly purified
to remove excess nucleotides and primers. This membrane-based system,
which can bind up to 40μg of DNA, recovers isolated DNA fragments or PCR
products in as little as 15 minutes, depending on the number of samples
processed. The purified DNA can be used for automated fluorescent DNA
sequencing, cloning, labeling, restriction enzyme digestion or in vitro
transcription/translation without further manipulation.
Features:
• Improved Productivity: Purify DNA fragments or PCR products in as little
as 15 minutes.
• Enhanced Cloning Results: Up to 95% recovery eluted in as little as
15μl.
• Confidence in Results: Purified DNA routinely achieves 700 bases with
>98% accuracy in automated fluorescent sequencing.
• Applications Tested: DNA is suitable for automated fluorescent
sequencing, cloning, labeling, restriction enzyme digestion or in vitro
transcription/translation without further manipulation.
• One System to Do It All: One system can replace up to four kits from
other suppliers.
Storage Conditions: Store at 22–25°C.
Protocol Part#
Wizard ® SV Gel and PCR Clean-Up System Technical Bulletin TB308
Size (bp)
9,416
3,199
1,000
500
100
PCR Product % Recovery
0 50 100
% Recovery
95%
95%
92%
89%
84%
Percent recovery of PCR products (100bp–9kb) from agarose gel
slices using the Wizard ® SV Gel and PCR Clean-Up System.
Total Colonies
2,000
1,800
1,600
1,400
1,200
1,000
800
Recombinant colonies
Nonrecombinant colonies
90%
Recombinants
600
67%
400
Recombinants
200
0 4382MA11_3A
Unpurified
Purified
Purification of PCR products enhances cloning success. A 500bp
PCR product was purified with the Wizard ® SV Gel and PCR Clean-Up
System and cloned into the pGEM ® -T Easy Vector. Both the percent
recombinants and total number of colonies increase with a pure PCR
product. White bars represent recombinant colonies. Blue bars represent
nonrecombinant colonies.
For complete and up-to-date product information visit: www.promega.com/catalog
4308MB11_3A