2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 104 pSP73 Vector Product Size Cat.# Price ($) pSP73 Vector 20 µg P2221 143.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pSP73 Vector offers a wide range of restriction sites, providing greater versatility in cloning and transcription of RNA in vitro. The pSP73 Vector contains the SP6 and T7 RNA polymerase promoters and a unique multiple cloning region, which includes restriction sites for BglII, EcoRV, ClaI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII, PvuII and XhoI. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning region. Features: • Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store vector at –20°C. Protocol Part# pSP73 Vector Technical Bulletin TB041 BglI 1330 XmnI 1809 ScaI 1690 PvuI 1580 FspI 1432 Amp r pSP73 Vector (2,464bp) ori SspI 2014 AatII 2132 NdeI 2381 HpaI 138 SP6 BglII EcoRV ClaI EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI PstI SphI HindIII PvuII XhoI T7 ➞➞ 1 start 6 14 19 24 34 40 42 45 51 57 58 67 73 75 83 87 103 0300VA05_2A Bacterial Strains and Competent Cells Product Bacterial Strain ES1301 mutS, Glycerol Stock Size Cat.# Price ($) (noncompetent) Bacterial Strain BMH 71-18 mutS, Glycerol Stock 200 µl Q6131 69.00 (noncompetent) 500 µl Q6321 69.00 Bacterial Strain JM109, Glycerol Stock 500 µl P9751 67.00 Bacterial Strain JM109(DE3), Glycerol Stock 500 µl P9801 67.00 Bacterial Strain LE392, Glycerol Stock 500 µl K9981 66.00 Bacterial Strain NM522, Glycerol Stock 500 µl P2301 67.00 Single Step (KRX) Competent Cells 20 × 50 µl L3002 330.00 L-Rhamnose Monohydrate 10 g L5701 70.00 50 g L5702 272.00 JM109 Competent Cells, >107cfu/µg 1 ml L1001 145.00 JM109 Competent Cells, >108cfu/µg 1 ml L2001 195.00 HB101 Competent Cells, >108cfu/µg 1 ml L2011 170.00 BL21(DE3)pLysS Competent Cells, >106cfu/µg 1 ml L1191 238.00 A. Normalized luminescence (RLU/OD) B. Induction Ratio 10,000 9,000 8,000 7,000 6,000 5,000 4,000 3,000 2,000 1,000 0 10,000 1,000 100 10 1 Normalized luminescence (RLU/OD) 14 140 120 100 80 60 40 20 0 BL21(DE3) BL21(DE3) pLysS For complete and up-to-date product information visit: www.promega.com/catalog 8 BL21(DE3) BL21(DE3) pLysS Pre-Induction Expression Roset t a 2 pLysS BL21(DE3) pLysS BL21(DE3) 43 Rosetta 2 pLysS KRX Rosetta 2 pLysS 1,700 KRX KRX Pre-induction and post-induction expression levels of firefly luciferase. Cells were transformed with the pF1K T7 Flexi ® Vector containing the firefly luciferase gene. Cultures were grown at 37°C to an optical density (O.D. 600) of 0.8–1.0 and then moved to a 25°C incubator shaker. When cultures reached an O.D. 600 of 1.0–1.5, protein expression was induced using either 0.1% rhamnose or 1mM IPTG and grown overnight at 25°C. Samples for luciferase assays were removed prior to and after induction. Panel A. Firefly luciferase expression level was determined using the Bright-Glo Luciferase Assay Reagent. Pre- and post-induction firefly luciferase expression levels were normalized to cell number (n = 3). Panel B. Induction ratios were calculated by dividing the post-induction luminescence values by the pre-induction values. 6032MA
Cell Signaling For complete and up-to-date product information visit: www.promega.com/catalog 5 DNA and RNA Purification DNA Fragment Purification 106 Genomic DNA Purification 110 Plasmid Purification 117 RNA Purification 124 DNA Quantitation 131 Nucleic Acid Purification Accessories 132 105 5 DNA and RNA Purification Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
Page 57 and 58: Cell Signaling SIRT-Glo Assays and
Page 59 and 60: Cell Signaling ADP-Glo Max Assay Pr
Page 61 and 62: Cell Signaling Kinase Enzyme System
Page 63 and 64: Cell Signaling Kinase Enzyme System
Page 65 and 66: Cell Signaling Kinase-Glo ® Kinase
Page 67 and 68: Cell Signaling SignaTECT ® Protein
Page 69 and 70: Cell Signaling Casein Kinase I Prod
Page 71 and 72: Cell Signaling Product Size Cat.# P
Page 73 and 74: Cell Signaling Protein Kinase Subst
Page 75 and 76: Cell Signaling For complete and up-
Page 77 and 78: Cell Signaling DNA Step Ladders Pro
Page 79 and 80: Cell Signaling Conventional DNA Mar
Page 81 and 82: Cell Signaling ProMega-Markers ® L
Page 83 and 84: Cell Signaling Restriction Enzymes
Page 85 and 86: Cell Signaling BamHI Product Size C
Page 87 and 88: Cell Signaling EcoRI Product Size C
Page 89 and 90: Cell Signaling NciI Product Size Co
Page 91 and 92: Cell Signaling SpeI Product Size Co
Page 93 and 94: Cell Signaling TSAP Thermosensitive
Page 95 and 96: Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98: Cell Signaling Kinases T4 Polynucle
Page 99 and 100: Cell Signaling S1 Nuclease Product
Page 101 and 102: Cell Signaling Percent Inhibition o
Page 103 and 104: Cell Signaling Untagged Flexi ® Ma
Page 105 and 106: Cell Signaling pGEM ® -5Zf(+) Vect
Page 107: Cell Signaling pSP64 Poly(A) Vector
Page 111 and 112: Cell Signaling x-tracta Gel Extract
Page 113 and 114: Cell Signaling Wizard ® MagneSil
Page 115 and 116: Cell Signaling ReliaPrep FFPE gDNA
Page 117 and 118: Cell Signaling MagneSil ® ONE, Fix
Page 119 and 120: Cell Signaling MagneSil ® KF, Geno
Page 121 and 122: Cell Signaling Plasmid Purification
Page 123 and 124: Cell Signaling PureYield Plasmid Ma
Page 125 and 126: Cell Signaling Wizard ® Plus Maxip
Page 127 and 128: Cell Signaling Wizard ® MagneSil
Page 129 and 130: Cell Signaling PureYield RNA Midipr
Page 131 and 132: Cell Signaling Maxwell ® 16 System
Page 133 and 134: Cell Signaling RNasin ® Plus RNase
Page 135 and 136: Cell Signaling DNA Quantitation Qua
Page 137 and 138: Cell Signaling Magnetic Stands and
Page 139 and 140: Cell Signaling 6 Drug Discovery For
Page 141 and 142: Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144: Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146: Cell Signaling Protease Assays Prot
Page 147 and 148: Cell Signaling Cell-Based Proteasom
Page 149 and 150: Cell Signaling Tryptase, Human, Rec
Page 151 and 152: Cell Signaling 7 Genetic Identity F
Page 153 and 154: Cell Signaling Maxwell ® 16 Forens
Page 155 and 156: Cell Signaling Plexor ® HY System
Page 157 and 158: Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Microbial Detection
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10 Instruments For complete and up-
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling Maxwell ® 16 System
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling dNTP Mix Product Siz
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Cell Signaling 12 PCR For complete
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Cell Signaling TaqBead Hot Start Po
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Cell Signaling GoTaq ® PCR Core Sy
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling Plexor ® qPCR and q
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Cell Signaling StemElite Differenti
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
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Cell Signaling HaloCHIP System Prod
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Cell Signaling MagneHis Protein Pur
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Cell Signaling Streptavidin Product
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Cell Signaling Protein Purification
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Cell Signaling 15 Reporter Assays a
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Cell Signaling Dual-Glo ® Lucifera
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Cell Signaling Firefly Luciferase R
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Cell Signaling Bright-Glo Luciferas
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Cell Signaling EnduRen Live Cell Su
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Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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