2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Cell Signaling<br />
pSP64 Poly(A) Vector<br />
Product Size Cat.# Price ($)<br />
pSP64 Poly(A) Vector 20 µg P1241 161.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The pSP64 Poly(A) Vector can be used as a standard cloning<br />
vector and for in vitro transcription from the SP6 promoter. The pSP64 Poly(A)<br />
Vector also can be used to generate poly(A)+ transcripts in vitro. The vector<br />
has a stretch of 30 dA:dT residues inserted between the SacI and EcoRI sites.<br />
Therefore, when foreign DNA is cloned into any polylinker site other than EcoRI<br />
(HindIII, PstI, SalI, AccI, HincII, XbaI, BamHI, AvaI, SmaI or SacI), linearization of<br />
the recombinant plasmid with EcoRI allows the use of SP6 RNA polymerase in<br />
vitro to prepare RNA copies of the inserted sequences that contain a synthetic<br />
3´ “poly(A)” tail of 30 residues.<br />
Features:<br />
• In Vitro Transcription: The SP6 promoter is next to the polylinker.<br />
• Generates Poly(A)+ Transcripts In Vitro: A stretch of 30 dA:dT<br />
residues are inserted between the SacI and EcoRI sites in the polylinker.<br />
Poly(A) tails can stabilize RNAs and lead to greater yields for in vitro<br />
translation reactions.<br />
• Convenient: Multiple cloning region provides a selection of restriction<br />
sites for cloning.<br />
Storage Conditions: Store vector at –20°C.<br />
Protocol Part#<br />
pSP64 Poly(A) Vector Technical Bulletin TB052<br />
ScaI<br />
1814<br />
PvuI<br />
1704<br />
FspI<br />
1556<br />
BglI<br />
1454<br />
SspI 2138<br />
XmnI 1933<br />
Amp r<br />
AatII 2256<br />
pSP64 Poly(A)<br />
Vector<br />
(3,030bp)<br />
ori<br />
SphI 2599<br />
NaeI 2758<br />
NheI 2789<br />
PvuII<br />
265<br />
HindIII<br />
PstI<br />
SalI<br />
AccI<br />
HincII<br />
XbaI<br />
BamHI<br />
AvaI<br />
SmaI<br />
SacI<br />
(dA:dT) 30<br />
EcoRI<br />
For complete and up-to-date product information visit: www.promega.com/catalog<br />
SP6<br />
➞<br />
1 start<br />
7<br />
20<br />
22<br />
23<br />
24<br />
28<br />
34<br />
39<br />
41<br />
50<br />
86<br />
0306VA05_2A<br />
pSP72 Vector<br />
Product Size Cat.# Price ($)<br />
pSP72 Vector<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
20 µg P2191 143.00<br />
Description: The pSP72 Vector can be used as a standard cloning vector and<br />
also can be used for transcription of RNA in vitro. The pSP72 Vector contains<br />
the SP6 and T7 RNA polymerase promoters flanking a unique multiple cloning<br />
region, which includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI,<br />
AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The pSP72<br />
and pSP73 Vectors are essentially identical except for the orientation of the<br />
multiple cloning site region.<br />
Features:<br />
• Versatile: This vector can be used for standard cloning and in vitro transcription<br />
from SP6 and T7 RNA polymerase promoters flanking the multiple<br />
cloning region.<br />
• Convenient: Multiple cloning site provides a selection of restriction sites<br />
for cloning.<br />
Storage Conditions: Store vector at –20°C.<br />
Protocol Part#<br />
pSP72 Vector Technical Bulletin TB040<br />
PvuI 1578 AatII 2130<br />
Amp<br />
pSP72<br />
Vector<br />
(2,462bp)<br />
r<br />
XmnI 1807<br />
ScaI 1688<br />
SspI <strong>2012</strong><br />
FspI<br />
430<br />
BglI<br />
1328<br />
NdeI<br />
2379<br />
ori<br />
HpaI<br />
136<br />
SP6<br />
XhoI<br />
PvuII<br />
HindIII<br />
SphI<br />
PstI<br />
SalI<br />
AccI<br />
XbaI<br />
BamHI<br />
SmaI<br />
KpnI<br />
SacI<br />
EcoRI<br />
ClaI<br />
EcoRV<br />
BglII<br />
T7<br />
➞➞<br />
1 start<br />
4<br />
12<br />
16<br />
26<br />
32<br />
34<br />
35<br />
40<br />
46<br />
53<br />
59<br />
65<br />
67<br />
74<br />
81<br />
85<br />
101<br />
0298VA05_2A<br />
103<br />
4<br />
Cloning and DNA Markers<br />
Section<br />
Contents<br />
Table of<br />
Contents
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