2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
102<br />
pGEM ® -9Zf(–) Vector<br />
Product Size Cat.# Price ($)<br />
pGEM ® -9Zf(–) Vector 20 µg P2391 158.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The pGEM ® -9Zf(–) Vector is a recombinant plasmid designed<br />
to provide a versatile range of cloning strategies, efficient synthesis of RNA<br />
in vitro and the production of single-stranded DNA. The plasmid contains T7<br />
and SP6 RNA polymerase promoters flanking a multiple cloning region within<br />
the α-peptide coding region of β-galactosidase. Insertional inactivation of the<br />
α-peptide allows recombinant clones to be identified directly by color screening<br />
on indicator plates when using appropriate E. coli strains (e.g., JM109). The<br />
multiple cloning region is unique and includes restriction sites for NsiI, SpeI,<br />
HindIII, XbaI, EcoRI, SalI and SacI.<br />
Features:<br />
• Excisable SP6/T7 Insert: This vector allows the excision of an insert<br />
containing the SP6 and T7 RNA polymerase promoters.<br />
• Blue/White Screening: Allows the easy identification of recombinant<br />
clones.<br />
• Versatile: This vector can be used for standard cloning, single-stranded<br />
DNA production and in vitro transcription from SP6 and T7 RNA polymerase<br />
promoters flanking the multiple cloning region.<br />
• Convenient: Multiple cloning site provides a selection of restriction sites<br />
for cloning.<br />
Storage Conditions: Store vector at –20°C and bacterial strain at –70°C.<br />
Protocol Part#<br />
pGEM ® -9Zf(–) Vector Technical Bulletin<br />
TB070<br />
XmnI 1935<br />
ScaI<br />
1816<br />
Amp r<br />
pGEM ® -9Zf(–)<br />
Vector<br />
(2,912bp)<br />
ori<br />
NaeI 2382<br />
f1 ori<br />
lacZ<br />
Tth111I<br />
NotI<br />
SP6<br />
NsiI<br />
SpeI<br />
HindIII<br />
XbaI<br />
EcoRI<br />
SalI<br />
SacI<br />
1 start<br />
9<br />
12<br />
18<br />
27<br />
36<br />
42<br />
52<br />
T7<br />
55<br />
SfiI 82<br />
➞<br />
➞<br />
2879<br />
2887<br />
0288VA05_4A<br />
pGEM ® -11Zf(+/–) Vectors<br />
Product Size Cat.# Price ($)<br />
pGEM ® -11Zf(+) Vector 20 µg P2411 158.00<br />
pGEM ® -11Zf(–) Vector<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
20 µg P2421 158.00<br />
Description: The pGEM ® -11Zf(+) and pGEM ® -11Zf(–) Vectors can be used<br />
as standard cloning vectors, as templates for in vitro transcription and for the<br />
production of ssDNA. These plasmids contain T7 and SP6 RNA polymerase<br />
promoters flanking a multiple cloning region within the α-peptide coding region<br />
of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant<br />
clones to be identified directly by color screening on indicator plates when<br />
using appropriate E. coli strains (e.g., JM109). The multiple cloning region<br />
contains unique restriction sites for SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI,<br />
XbaI, NotI, SphI, NsiI and HindIII. The pGEM ® -11Zf(–) and pGEM ® -11Zf(+) Vectors<br />
are identical except for the orientation of the f1 origin.<br />
Features:<br />
• Blue/White Screening: Allows the easy identification of recombinant<br />
clones.<br />
• Versatile: These vectors can be used for standard cloning, single-stranded<br />
DNA production and in vitro transcription from SP6 and T7 RNA polymerase<br />
promoters flanking the multiple cloning region.<br />
• Convenient: Multiple cloning site provides a selection of restriction sites<br />
for cloning.<br />
Storage Conditions: Store vector at –20°C and bacterial strain at –70°C.<br />
Protocol Part#<br />
pGEM ® -11Zf(+) Vector Technical Bulletin<br />
TB075<br />
pGEM ® -11Zf(–) Vector Technical Bulletin<br />
TB074<br />
XmnI<br />
1961<br />
ScaI<br />
1842<br />
Amp r<br />
AatII<br />
2284<br />
NdeI<br />
2533<br />
pGEM ® -11Zf(+/–)<br />
Vectors<br />
(3,221bp)<br />
For complete and up-to-date product information visit: www.promega.com/catalog<br />
ori<br />
f1 ori<br />
lacZ<br />
T7<br />
SfiI<br />
SacI<br />
EcoRI<br />
SalI<br />
XhoI<br />
BamHI<br />
ApaI<br />
XbaI<br />
NotI<br />
SphI<br />
NsiI<br />
HindIII<br />
➞➞<br />
SP6<br />
1 start<br />
17<br />
27<br />
29<br />
35<br />
41<br />
47<br />
57<br />
59<br />
67<br />
76<br />
78<br />
80<br />
93<br />
0290VA05_4A
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