2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Cell Signaling<br />
pGEM ® -5Zf(+) Vector<br />
Product Size Cat.# Price ($)<br />
pGEM ® -5Zf(+) Vector 20 µg P2241 158.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The pGEM ® -5Zf(+) Vector is derived from the pGEM ® -3Zf(+)<br />
Vector and contains the origin of replication of the filamentous phage f1.<br />
This plasmid serves as a standard cloning vector, as a template for in vitro<br />
transcription and can be used for the production of circular ssDNA. This vector<br />
contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning<br />
region within the α-peptide coding region of β-galactosidase. Insertional<br />
inactivation of the α-peptide allows recombinant clones to be identified directly<br />
by color screening on indicator plates when using appropriate E. coli strains<br />
(e.g., JM109). The multiple cloning region contains unique restriction sites for<br />
ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and<br />
NsiI. This arrangement is designed specifically for generating unidirectional<br />
deletions with the Erase-a-Base System.<br />
Features:<br />
• Blue/White Screening: Allows the easy identification of recombinant<br />
clones.<br />
• Versatile: This vector can be used for standard cloning, single-stranded<br />
DNA production and in vitro transcription from SP6 and T7 RNA polymerase<br />
promoters flanking the multiple cloning region.<br />
• Convenient: Multiple cloning site provides a selection of restriction sites<br />
for cloning.<br />
• Unidirectional Deletions: Restriction sites are positioned conveniently for<br />
use with the Erase-a-Base System.<br />
Storage Conditions: Store vector at –20°C and bacterial strain at –70°C.<br />
Protocol Part#<br />
pGEM ® -5Zf(+) Vector Technical Bulletin<br />
TB047<br />
XmnI 1994<br />
ScaI<br />
1875<br />
Amp r<br />
pGEM ® -5Zf(+/–)<br />
Vectors<br />
(3,000bp)<br />
ori<br />
f1 ori<br />
lacZ<br />
ApaI<br />
AatII<br />
SphI<br />
NcoI<br />
SacII<br />
EcoRV<br />
SpeI<br />
NotI<br />
PstI<br />
SalI<br />
NdeI<br />
SacI<br />
BstXI<br />
NsiI<br />
For complete and up-to-date product information visit: www.promega.com/catalog<br />
T7<br />
➞<br />
➞<br />
SP6<br />
1 start<br />
14<br />
20<br />
26<br />
37<br />
46<br />
51<br />
55<br />
62<br />
73<br />
75<br />
82<br />
94<br />
103<br />
112<br />
126<br />
0284VA05_4A<br />
pGEM ® -7Zf(+/–) Vectors<br />
Product Size Cat.# Price ($)<br />
pGEM ® -7Zf(+) Vector 20 µg P2251 158.00<br />
pGEM ® -7Zf(–) Vector<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
20 µg P2371 158.00<br />
Description: The pGEM ® -7Zf(+) and pGEM ® -7Zf(–) Vectors are derivatives<br />
of the pGEM ® -3Zf(+) Vector and contain the origin of replication of the<br />
filamentous phage f1. These plasmids serve as standard cloning vectors,<br />
as templates for in vitro transcription and can be used for the production of<br />
circular ssDNA. These plasmids contain SP6 and T7 RNA polymerase<br />
promoters flanking a region of multiple cloning sites within the α-peptide<br />
coding region of β-galactosidase. Insertional inactivation of the α-peptide<br />
allows recombinant clones to be identified directly by color screening on<br />
indicator plates when using appropriate E. coli strains (e.g., JM109). The<br />
multiple cloning region is unique and includes restriction sites for ApaI, AatII,<br />
SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, Csp45I, ClaI, HindIII, BamHI, SacI, BstXI and<br />
NsiI. This arrangement is designed specifically for generating unidirectional<br />
deletions with the Erase-a-Base System. pGEM ® -7Zf(+) and pGEM ® -7Zf(–)<br />
Vectors are identical except for the orientation of the f1 origin.<br />
Features:<br />
• Blue/White Screening: Allows the easy identification of recombinant<br />
clones.<br />
• Versatile: These standard cloning vectors are equipped for singlestranded<br />
DNA production and in vitro transcription from SP6 and T7 RNA<br />
polymerase promoters flanking the multiple cloning region.<br />
• Convenient: Multiple cloning site provides a selection of restriction sites<br />
for cloning.<br />
• Unidirectional Deletions: Restriction sites are positioned conveniently for<br />
use with the Erase-a-Base System.<br />
Storage Conditions: Store vector at –20°C and bacterial strain at –70°C.<br />
Protocol Part#<br />
pGEM ® -7Zf(+) Vector Technical Bulletin<br />
TB048<br />
pGEM ® -7Zf(–) Vector Technical Bulletin<br />
TB069<br />
XmnI 1991<br />
ScaI<br />
1872<br />
Amp r<br />
pGEM ® -7Zf(+/–)<br />
Vectors<br />
(2,997bp)<br />
ori<br />
f1 ori<br />
lacZ<br />
T7<br />
ApaI<br />
AatII<br />
SphI<br />
XbaI<br />
XhoI<br />
EcoRI<br />
KpnI<br />
SmaI<br />
Csp45I<br />
ClaI<br />
HindIII<br />
BamHI<br />
SacI<br />
BstXI<br />
NsiI<br />
➞➞<br />
SP6<br />
1 start<br />
14<br />
20<br />
26<br />
31<br />
37<br />
43<br />
53<br />
56<br />
61<br />
67<br />
72<br />
78<br />
91<br />
100<br />
109<br />
123<br />
0286VA05_4A<br />
101<br />
4<br />
Cloning and DNA Markers<br />
Section<br />
Contents<br />
Table of<br />
Contents
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