2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 100 pGEM ® -3Zf(+/–) Vectors Product Size Cat.# Price ($) pGEM ® -3Zf(+) Vector 20 µg P2271 158.00 pGEM ® -3Zf(–) Vector 20 µg P2261 158.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM ® -3Zf(+) and pGEM ® -3Zf(–) Vectors are derived from the pGEM ® -3Z Vector and contain the origin of replication of the filamentous phage f1. These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII. The pGEM ® -3Zf(+) and -3Zf(–) Vectors are identical except for the orientation of the f1 origin and can be used as standard cloning vectors, as templates for in vitro transcription and for the production of circular ssDNA. Features: • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: These vectors can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store vector at –20°C and bacterial strain at –70°C. Protocol Part# pGEM ® -3Zf(+) Vector Technical Bulletin TB086 pGEM ® -3Zf(–) Vector Technical Bulletin TB045 XmnI 1937 ScaI 1818 Amp r AatII 2260 NdeI 2509 pGEM ® -3Zf(+/–) Vectors (3,197bp) ori f1 ori lacZ T7 EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII ➞➞ SP6 1 start 5 15 21 21 23 26 32 38 39 40 48 54 56 69 0282VA05_4A pGEM ® -4Z Vector Product Size Cat.# Price ($) pGEM ® -4Z Vector For Research Use Only. Not for Use in Diagnostic Procedures. 20 µg P2161 143.00 Description: The pGEM ® -4Z Vector is intended for use as a standard cloning vector, as well as for the highly efficient synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. The pGEM ® -3Z and pGEM ® -4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters. Features: • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. Storage Conditions: Store vector at –20°C and bacterial strain at –70°C. Protocol Part# pGEM ® -4Z Vector Technical Bulletin TB036 XmnI 1939 SspI 2144 ScaI 1820 AatII 2262 Amp r pGEM ® -4Z Vector (2,746bp) NdeI 2511 NarI 2563 For complete and up-to-date product information visit: www.promega.com/catalog ori lacZ SP6 EcoRI SacI KpnI AvaI XmaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII T7 ➞➞ 1 start 7 17 23 23 23 25 28 34 40 41 42 50 56 58 70 0280VA05_4A
Cell Signaling pGEM ® -5Zf(+) Vector Product Size Cat.# Price ($) pGEM ® -5Zf(+) Vector 20 µg P2241 158.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pGEM ® -5Zf(+) Vector is derived from the pGEM ® -3Zf(+) Vector and contains the origin of replication of the filamentous phage f1. This plasmid serves as a standard cloning vector, as a template for in vitro transcription and can be used for the production of circular ssDNA. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base System. Features: • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: This vector can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. • Unidirectional Deletions: Restriction sites are positioned conveniently for use with the Erase-a-Base System. Storage Conditions: Store vector at –20°C and bacterial strain at –70°C. Protocol Part# pGEM ® -5Zf(+) Vector Technical Bulletin TB047 XmnI 1994 ScaI 1875 Amp r pGEM ® -5Zf(+/–) Vectors (3,000bp) ori f1 ori lacZ ApaI AatII SphI NcoI SacII EcoRV SpeI NotI PstI SalI NdeI SacI BstXI NsiI For complete and up-to-date product information visit: www.promega.com/catalog T7 ➞ ➞ SP6 1 start 14 20 26 37 46 51 55 62 73 75 82 94 103 112 126 0284VA05_4A pGEM ® -7Zf(+/–) Vectors Product Size Cat.# Price ($) pGEM ® -7Zf(+) Vector 20 µg P2251 158.00 pGEM ® -7Zf(–) Vector For Research Use Only. Not for Use in Diagnostic Procedures. 20 µg P2371 158.00 Description: The pGEM ® -7Zf(+) and pGEM ® -7Zf(–) Vectors are derivatives of the pGEM ® -3Zf(+) Vector and contain the origin of replication of the filamentous phage f1. These plasmids serve as standard cloning vectors, as templates for in vitro transcription and can be used for the production of circular ssDNA. These plasmids contain SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, Csp45I, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base System. pGEM ® -7Zf(+) and pGEM ® -7Zf(–) Vectors are identical except for the orientation of the f1 origin. Features: • Blue/White Screening: Allows the easy identification of recombinant clones. • Versatile: These standard cloning vectors are equipped for singlestranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. • Convenient: Multiple cloning site provides a selection of restriction sites for cloning. • Unidirectional Deletions: Restriction sites are positioned conveniently for use with the Erase-a-Base System. Storage Conditions: Store vector at –20°C and bacterial strain at –70°C. Protocol Part# pGEM ® -7Zf(+) Vector Technical Bulletin TB048 pGEM ® -7Zf(–) Vector Technical Bulletin TB069 XmnI 1991 ScaI 1872 Amp r pGEM ® -7Zf(+/–) Vectors (2,997bp) ori f1 ori lacZ T7 ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI Csp45I ClaI HindIII BamHI SacI BstXI NsiI ➞➞ SP6 1 start 14 20 26 31 37 43 53 56 61 67 72 78 91 100 109 123 0286VA05_4A 101 4 Cloning and DNA Markers Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
Page 53 and 54: Cell Signaling cAMP-Glo Max Assay P
Page 55 and 56: Cell Signaling Growth Factors Epide
Page 57 and 58: Cell Signaling SIRT-Glo Assays and
Page 59 and 60: Cell Signaling ADP-Glo Max Assay Pr
Page 61 and 62: Cell Signaling Kinase Enzyme System
Page 63 and 64: Cell Signaling Kinase Enzyme System
Page 65 and 66: Cell Signaling Kinase-Glo ® Kinase
Page 67 and 68: Cell Signaling SignaTECT ® Protein
Page 69 and 70: Cell Signaling Casein Kinase I Prod
Page 71 and 72: Cell Signaling Product Size Cat.# P
Page 73 and 74: Cell Signaling Protein Kinase Subst
Page 75 and 76: Cell Signaling For complete and up-
Page 77 and 78: Cell Signaling DNA Step Ladders Pro
Page 79 and 80: Cell Signaling Conventional DNA Mar
Page 81 and 82: Cell Signaling ProMega-Markers ® L
Page 83 and 84: Cell Signaling Restriction Enzymes
Page 85 and 86: Cell Signaling BamHI Product Size C
Page 87 and 88: Cell Signaling EcoRI Product Size C
Page 89 and 90: Cell Signaling NciI Product Size Co
Page 91 and 92: Cell Signaling SpeI Product Size Co
Page 93 and 94: Cell Signaling TSAP Thermosensitive
Page 95 and 96: Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98: Cell Signaling Kinases T4 Polynucle
Page 99 and 100: Cell Signaling S1 Nuclease Product
Page 101 and 102: Cell Signaling Percent Inhibition o
Page 103: Cell Signaling Untagged Flexi ® Ma
Page 107 and 108: Cell Signaling pSP64 Poly(A) Vector
Page 109 and 110: Cell Signaling For complete and up-
Page 111 and 112: Cell Signaling x-tracta Gel Extract
Page 113 and 114: Cell Signaling Wizard ® MagneSil
Page 115 and 116: Cell Signaling ReliaPrep FFPE gDNA
Page 117 and 118: Cell Signaling MagneSil ® ONE, Fix
Page 119 and 120: Cell Signaling MagneSil ® KF, Geno
Page 121 and 122: Cell Signaling Plasmid Purification
Page 123 and 124: Cell Signaling PureYield Plasmid Ma
Page 125 and 126: Cell Signaling Wizard ® Plus Maxip
Page 127 and 128: Cell Signaling Wizard ® MagneSil
Page 129 and 130: Cell Signaling PureYield RNA Midipr
Page 131 and 132: Cell Signaling Maxwell ® 16 System
Page 133 and 134: Cell Signaling RNasin ® Plus RNase
Page 135 and 136: Cell Signaling DNA Quantitation Qua
Page 137 and 138: Cell Signaling Magnetic Stands and
Page 139 and 140: Cell Signaling 6 Drug Discovery For
Page 141 and 142: Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144: Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146: Cell Signaling Protease Assays Prot
Page 147 and 148: Cell Signaling Cell-Based Proteasom
Page 149 and 150: Cell Signaling Tryptase, Human, Rec
Page 151 and 152: Cell Signaling 7 Genetic Identity F
Page 153 and 154: Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Microbial Detection
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10 Instruments For complete and up-
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling Maxwell ® 16 System
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling dNTP Mix Product Siz
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Cell Signaling 12 PCR For complete
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Cell Signaling TaqBead Hot Start Po
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Cell Signaling GoTaq ® PCR Core Sy
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling Plexor ® qPCR and q
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Cell Signaling StemElite Differenti
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
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Cell Signaling HaloCHIP System Prod
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Cell Signaling MagneHis Protein Pur
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Cell Signaling Streptavidin Product
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Cell Signaling Protein Purification
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Cell Signaling 15 Reporter Assays a
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Cell Signaling Dual-Glo ® Lucifera
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Cell Signaling Firefly Luciferase R
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Cell Signaling Bright-Glo Luciferas
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Cell Signaling EnduRen Live Cell Su
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Cell Signaling Beta-Glo ® Assay Sy
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Cell Signaling Promoter-Driven Cont
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Cell Signaling Nuclear Receptor Pat
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Cell Signaling pRL Renilla Lucifera
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Cell Signaling GloResponse Lucifera
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Cell Signaling pSP-luc+NF Fusion Ve
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Transfection Reagent
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Cell Signaling 16 RNA Analysis For
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Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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