2012 Promega catalogue

Cell Signaling
Untagged Flexi ® Mammalian Expression
Vectors
Product Size Cat.# Price ($)
pF4A CMV Flexi ® Vector 20 µg C8481 489.00
pF4K CMV Flexi ® Vector 20 µg C8491 489.00
pF5A CMV-neo Flexi ® Vector 20 µg C9401 489.00
pF5K CMV-neo Flexi ® Vector 20 µg C9411 489.00
pF9A CMV hRluc-neo Flexi ® Vector 20 µg C9361 489.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: These Flexi ® Vectors are designed specifically for high-level
expression of proteins in mammalian cells from the CMV promoter with or
without a selectable marker. The pFN9A Vector provides Renilla luciferase,
which may be used as a transfection control. The pFN9A Vector was designed
to complement pGL4 firefly luciferase vectors when exogenous proteins (e.g.,
a receptor of transcription factor) must be expressed for reporter assays.
All inserts may be confirmed by cell-free expression with the TnT ® T7 Quick
System (Cat.# L1170).
Note: Flexi ® Vectors contain the lethal barnase gene to reduce background
colonies without inserts during the subcloning procedure. Using the Flexi ® Vector
Cloning System replaces the barnase gene with your insert. These
vectors, as purchased, cannot be cultured in normal laboratory strains of
E. coli without an insert.
Features:
• Versatility: You can choose between a variety of initial applications
(e.g., bacterial protein, mammalian, or cell-free protein expression)
and then transfer to others as required.
• Time Savings: Efficient transfer allows for direct use of recombinant
clones, minimizing time wasted screening background colonies.
• Enhanced Productivity: Adaptable to high-throughput formats for large
screening projects.
• Easy Access: No licensing fees or complicated transfer restrictions.
Storage Conditions: Store vectors at –20°C.
Inducible T7-Driven Flexi ® Vectors for E. coli
Expression
Product Size Cat.# Price ($)
pF1A T7 Flexi ® Vector 20 µg C8441 435.00
pF1K T7 Flexi ® Vector 20 µg C8451 435.00
pFN18A HaloTag ® T7 Flexi ® Vector 20 µg G2751 397.00
pFN18K HaloTag ® T7 Flexi ® Vector 20 µg G2681 397.00
pFN19A HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1891 397.00
pFN19K HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1841 397.00
pFC20A HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1681 397.00
pFC20K HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1691 397.00
pFN2A (GST) Flexi ® Vector 20 µg C8461 489.00
pFN2K (GST) Flexi ® Vector 20 µg C8471 489.00
pFN6A (HQ) Flexi ® Vector 20 µg C8511 489.00
pFN6K (HQ) Flexi ® Vector 20 µg C8521 489.00
pFC7A (HQ) Flexi ® Vector 20 µg C8531 489.00
pFC7K (HQ) Flexi ® Vector
For Research Use Only. Not for Use in Diagnostic Procedures.
20 µg C8541 489.00
For additional information see page 237.
For complete and up-to-date product information visit: www.promega.com/catalog
pALTER ® -MAX Vector
Product Size Cat.# Price ($)
pALTER ® -MAX Vector
Available Separately
20 µg Q5761 510.00
Ampicillin Repair Oligonucleotide
For Research Use Only. Not for Use in Diagnostic Procedures.
30 µl Q6311 93.00
Description: The pALTER ® -MAX Vector is a 5,534bp plasmid. It contains the
human cytomegalovirus (CMV) immediate-early enhancer/promoter region for
strong, constitutive expression of cloned DNA inserts in a variety of mammalian
cell types. The pALTER ® -MAX Vector as supplied is chloramphenicol-resistant
and ampicillin-sensitive.
Features:
• High Expression: Protein expression driven by the human cytomegalovirus
(CMV) immediate-early enhancer/promoter region allows high
constitutive expression in a variety of cell lines. A chimeric intron composed
of the 5´-splice site from the β-globin intron and the 3´-splice site from an
IgG intron allows increased expression levels.
• Efficient: SV40 late polyadenylation signal for efficient RNA processing. T7
and T3 RNA polymerase promoters flank the multiple cloning site and allow
RNA in vitro synthesis from sense or antisense strands.
• Flexible: Restriction sites flanking the CMV enhancer (BglII and SgfI) and
the CMV promoter (SgfI and I-PpoI) allow for easy replacement of these
regions by alternative regulatory regions.
Storage Conditions: Store vector DNA at –20°C.
pGEM ® -3Z Vector
Product Size Cat.# Price ($)
pGEM ® -3Z Vector
For Research Use Only. Not for Use in Diagnostic Procedures.
20 µg P2151 143.00
Description: The pGEM ® -3Z Vector is intended for use as a standard cloning
vector, as well as for the highly efficient synthesis of RNA in vitro. The vector
carries the lacZ α-peptide and the multiple cloning region arrangement from
pUC18 allowing selection of recombinants by blue/white screening. In addition,
the vector contains both the SP6 and T7 RNA polymerase promoters flanking
the multiple cloning region.
The pGEM ® -3Z and pGEM ® -4Z Vectors are essentially identical except for the
orientation of the SP6 and T7 promoters.
Features:
• Blue/White Screening: Allows the easy identification of recombinant
clones.
• Versatile: This vector can be used for standard cloning and in vitro
transcription from SP6 and T7 RNA polymerase promoters flanking the
multiple cloning region.
• Convenient: Multiple cloning site provides a selection of restriction sites
for cloning.
Storage Conditions: Store vector at –20°C.
Protocol Part#
pGEM ® -3Z Vector Technical Bulletin
TB033
XmnI 1937
ScaI 1818
Amp r
SspI
2142
pGEM ® -3Z
Vector
(2,743bp)
ori
AatII
2260
lacZ
NdeI
2509
NarI
2561
T7
EcoRI
SacI
KpnI
AvaI
XmaI
SmaI
BamHI
XbaI
SalI
AccI
HincII
PstI
SphI
HindIII
➞➞
SP6
1 start
5
15
21
21
21
23
26
32
38
39
40
48
54
56
69
0278VA05_4A
99
4
Cloning and DNA Markers
Section
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