2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
98
Flexi ® Cloning System
Product Size Cat.# Price ($)
Flexi ® System, Entry/
Transfer 5 entry and 20 transfer reactions C8640 435.00
Flexi ® System,
Transfer 100 transfer reactions C8820 1730.00
Carboxy Flexi ®
System, Transfer 50 transfer reactions C9320 758.00
Available Separately
10X Flexi ® Enzyme Blend (SgfI & PmeI) 25 µl R1851 166.00
100 µl R1852 585.00
Carboxy Flexi ® Enzyme Blend (SgfI & EcoICRI) 50 µl R1901 165.00
HaloTag ® Cloning Starter System
For Research Use Only. Not for Use in Diagnostic Procedures.
1 each G6050 568.00
Description: The Flexi ® Vector System is a directional cloning method for
protein-coding sequences. It is based on two rare-cutting restriction enzymes,
SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer
protein-coding regions without the need to resequence.
All Flexi ® Vectors carry the lethal barnase gene, which is replaced by the DNA
fragment of interest and acts as a positive selection for the successful ligation
of the insert.
Unlike site-specific recombination vector systems, the Flexi ® Vector Systems do
not require appending multiple amino acids to the amino or carboxy termini of
the protein of interest. In addition, the systems do not require an archival entry
vector, and most applications allow direct entry into the vector suited to the
experimental design.
C-terminal Flexi ® Vectors allow expression of C-terminal-tagged proteins.
While these vectors can act as acceptors of protein-coding regions flanked by
SgfI and PmeI, they lack a PmeI site and contain a different blunt-ended site,
EcoICRI. This joined sequence cannot be removed from the C-terminal Flexi ®
Vectors and transferred to other Flexi ® Vectors.
Features:
• Versatility: You can choose between a variety of initial applications (e.g.,
bacterial protein, mammalian, or cell-free protein expression) and then
transfer to others as required.
• Time Savings: Efficient transfer allows for direct use of recombinant
clones, minimizing time wasted screening background colonies.
• Enhanced Productivity: Adaptable to high-throughput formats for large
screening projects.
• Easy Access: No licensing fees or complicated transfer restrictions.
Storage Conditions: Cat.# C8640 is comprised of Cat.# C8641 and A9280.
Store Cat.# C8641 at –20°C; store Cat.# A9280 at room temperature. Store
Cat.# C8820 and C9320 at –20°C. Store enzyme blends at –20°C.
Protocol Part#
Flexi ® Vector Systems Technical Manual
TM254
SgfI
PCR
SgfI PmeI
DNA Purification
DNA Purification
SgfI PmeI
Ligation
Transformation
and Selection
protein-coding region
protein-coding region
For complete and up-to-date product information visit: www.promega.com/catalog
PmeI
Digestion with Flexi ®
Enzyme Blend
SgfI PmeI
lethal gene
lethal gene
SgfI PmeI
Acceptor
Flexi ®
Vector
Digestion with Flexi ®
Enzyme Blend
4599MA