2012 Promega catalogue

Cell Signaling
Percent Inhibition of RNase Activity
RNase A Activity (units)
90
80
70
60
50
40
30
20
10
0
0.25
0.2
0.15
0.1
0.05
4u RNasin ®
0
4u Superase•In
40u Superase•In
400u Superase•In
Amount and Type of Inhibitor Added
RNase A
RNase A + 8u RNasin ®
Ribonuclease Inhibitor
pre-incubation
in assay
RNase A +
8u Superase•In
RNase A +
40u Superase•In
Comparison of RNasin ® Ribonuclease Inhibitor and Superase•In
inhibition of RNase A activity. Panel A. Total yeast RNA assay. Total yeast
RNA was incubated in the presence of 5ng RNase A for 5 minutes at 37°C
in 0.5ml of reaction mix containing 50mM MOPS and 5mM MgCl 2 (pH 6.5).
The indicated amounts of inhibitor (RNasin ® or Superase•In) were mixed
with the RNA prior to RNase addition. After incubation, 0.5ml 10% TCA
was added to stop the reaction and to precipitate the large RNA molecules.
An OD 280 measurement was taken of the TCA-soluble material. Panel B.
“Pre-incubation” and “in assay” conditions. The total yeast RNA assay was
performed as described in Panel A along with an experimental modification
of “pre-incubation.” For the pre-incubation assay, the ribonuclease inhibitors
were mixed with RNase and incubated for 15 minutes at 22°C. The
pre-incubation mix was then added to the RNA.
For complete and up-to-date product information visit: www.promega.com/catalog
3109MA10_0A
3074MA09_0A
Subcloning Tools and Vectors
Subcloning Tools Bundle
Product Size Cat.# Price ($)
Subcloning Tools Bundle 1 each M1060 495.00
For Research Use Only. Not for Use in Diagnostic Procedures. Product may not be available in
all countries. Please contact your local representative for more information.
Description: Speed your subcloning with these easy-to-use tools. Purchase
the Subcloning Tools Bundle, and get LigaFast Rapid DNA Ligation System,
TSAP Thermosensitive Alkaline Phosphatase, BenchTop 100bp DNA Ladder,
Wizard ® SV Gel and PCR Clean-Up System and PureYield Plasmid Miniprep
System for one low price. It’s like getting the PureYield Plasmid Miniprep
System for free.
Features:
• LigaFast Rapid DNA Ligation System: Rapid room temperature
ligations of vectors and inserts in as little as 5 minutes. Transform
competent bacteria immediately following the reaction. See LigaFast
Rapid DNA Ligation System Product Information #9PIM822 for protocol.
• TSAP Thermosensitive Alkaline Phosphatase: Use rapid protocol
(included) to digest and dephosphorylate at the same time or use in
standard application. Heat kill the enzyme after the reaction in 15 minutes.
Active in common restriction enzyme buffers with no zinc requirement.
See TSAP Thermosensitive Alkaline Phosphatase Product Information
#9PIM991 for protocol.
• BenchTop 100bp DNA Ladder: Ready-to-load marker for agarose gel
electrophoresis. Use when gel purifying either vector or insert.
• Wizard ® SV Gel and PCR Clean-Up System: Rapid gel purification of
fragments for 100bp to 10kb. Great for removing enzymes from DNA as
well. High-capacity and low elution volume. See Wizard ® SV Gel and PCR
Clean-Up System Technical Bulletin #TB308 for protocol.
• PureYield Plasmid Miniprep System: Rapid 10-minute miniprep.
Prepare your vector for subcloning or use to screen for recombinants.
Go from screening to transfection thanks to the high-quality DNA.
See PureYield Plasmid Miniprep System Technical Bulletin #TB374 for
protocol.
Storage Conditions: Store the LigaFast Rapid DNA Ligation System
(M8221) and TSAP Thermosensitive Alkaline Phosphatase (M9910) at –20°C.
Store the BenchTop 100bp DNA Ladder at 22–25°C; storage at –20°C can
enhance the shelf life of this product. Store the Wizard ® SV Gel and PCR
Clean-Up System (A9281) and PureYield Plasmid Miniprep System (A1223)
at 22–25°C.
97
4
Cloning and DNA Markers
Section
Contents
Table of
Contents