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2012 Promega catalogue

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Life<br />

Science<br />

Catalog<br />

<strong>2012</strong><br />

Worldwide Contact List<br />

Section<br />

Contents<br />

Table of<br />

Contents<br />

Cell Signaling<br />

6<br />

EDTA, 0.5M (pH 8.0), Molecular Biology Grade<br />

Product Size Cat.# Price ($)<br />

EDTA, 0.5M (pH 8.0), Molecular Biology Grade 100 ml V4231 32.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

400 ml V4233 113.00<br />

Description: EDTA, 0.5M (pH 8.0), Molecular Biology Grade, is a chelator<br />

of divalent cations and is suitable for biochemistry and molecular biology<br />

applications. It is supplied as a solution in deionized water.<br />

Form: Clear, colorless liquid.<br />

Properties:<br />

• pH at 25°C: 7.9–8.1.<br />

• A 280 at 0.5M: ≤0.25.<br />

• RNase Activity at 0.5M: ≤1.0% release of 3 H-RNA.<br />

• DNase Activity at 0.5M: ≤1.0% release of 3 H-DNA.<br />

• Protease Assay: None detected.<br />

Storage Conditions: Store at 22–25°C.<br />

EDTA, Disodium Salt (Dihydrate), Molecular<br />

Biology Grade<br />

Product Size Cat.# Price ($)<br />

EDTA, Disodium Salt, Molecular Biology Grade 100 g H5031 47.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

500 g H5032 172.00<br />

Description: EDTA, Disodium Salt, Molecular Biology Grade, is a chelator of<br />

divalent metal cations.<br />

Formula Weight: 372.20.<br />

Properties:<br />

• Purity: ≥99.0%.<br />

• Insolubles: ≤0.005%.<br />

• Lead: ≤5ppm.<br />

• Iron: ≤10ppm.<br />

Features:<br />

• Quality Tested: Each lot of EDTA is tested and certified to be free of<br />

DNase, RNase and protease activity.<br />

Storage Conditions: Store at 22–25°C.<br />

Ethidium Bromide Solution, Molecular Grade<br />

Product<br />

Ethidium Bromide Solution, Molecular<br />

Size Conc. Cat.# Price ($)<br />

Grade 10 ml 10 mg/ml H5041 87.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

Description: Ethidium Bromide Solution, Molecular Grade (10mg/ml), is a<br />

fluorescent dye suitable for staining nucleic acids after electrophoresis or in<br />

cesium chloride gradients. The solution can be used to detect both doublestranded<br />

and single-stranded DNA.<br />

Features:<br />

• Quality Tested: Each lot of Ethidium Bromide Solution is tested and<br />

certified to be free of DNase, RNase and protease activity.<br />

Storage Conditions: Store at 22–25°C.<br />

Formamide, Molecular Grade<br />

Product Size Cat.# Price ($)<br />

Formamide, Molecular Grade 100 ml H5051 106.00<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

500 ml H5052 256.00<br />

Description: Formamide is often used for the denaturation of nucleic acids in<br />

applications such as hybridization, sequencing gel electrophoresis and electron<br />

microscopy.<br />

Formula Weight: 45.04.<br />

Properties:<br />

• Purity: ≥99.5%.<br />

• Copper: ≤1ppm.<br />

• Iron: ≤1ppm.<br />

• Lead: ≤1ppm.<br />

• Zinc: ≤1ppm.<br />

• Refractive Index at 20°C: 1.446–1.448.<br />

• pH at 25°C of 1%: 6.5–7.5.<br />

• A 260 at 10%: ≤0.10.<br />

• A 280 at 10%: ≤0.02.<br />

Features:<br />

• Quality Tested: Each lot of Formamide is tested and certified to be free of<br />

DNase, RNase and protease activity.<br />

Storage Conditions: Store at 22–25°C.<br />

Glycerol, Molecular Biology Grade<br />

Product Size Cat.# Price ($)<br />

Glycerol, Molecular Biology Grade<br />

For Research Use Only. Not for Use in Diagnostic Procedures.<br />

1,000 ml H5433 102.00<br />

Description: Glycerol is used for storage of enzymes at low temperatures. A<br />

50% (w/v) glycerol solution will not freeze at –20°C. Glycerol is often used as a<br />

component in electrophoresis loading buffers because of its density (1.26g/ml).<br />

In addition, glycerol gradients can be used in the purification of bacteriophage<br />

or proteins. Cat.# H5433 is anhydrous glycerol with a purity of ≥99.5%.<br />

Properties:<br />

• Purity: ≥99.5%.<br />

• Calcium: ≤2ppm.<br />

• Magnesium: ≤1ppm.<br />

• Lead: ≤5ppm.<br />

• Zinc: ≤1ppm.<br />

• A 260 at 10%: ≤0.05.<br />

• A 280 at 10%: ≤0.05.<br />

Features:<br />

• Quality Tested: Each lot of glycerol is tested and certified to be free of<br />

DNase, RNase and protease activity.<br />

Storage Conditions: Store at 22–25°C.<br />

For complete and up-to-date product information visit: www.promega.com/catalog

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