Development of a Bioluminescent Cell-Based Assay to ... - Promega

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Cells as reagents in bioassays Frozen, Thaw-and-Use Cells 1. Human cell lines - Developed as Thaw-and-Use for immediate use in bioassay - Designed to give good recovery and robust response upon thawing 2. Thaw-and-Use format - Cell propagation conditions & defined freezing protocol control assay performance for a consistent bioassay response - No pre-culturing prior to assay means less variability introduced (overgrown, etc.) - Indefinite storage - Identical cells in bioassay, day-to-day 3. Minimizes pre-assay planning, time & labor - Ample cell banks provide long-term supply Confidential and Proprietary. Not for Further Disclosure. 6
FcgRIIIa antibody blocking of ADCC reporter bioassay response demonstrates FcgRIIIa expression Confidential and Proprietary. Not for Further Disclosure. Effector cell line is a Jurkat cell line engineered to express FcgRIIIa (V158) receptor and NFAT-RE luc2 luciferase Assay used WIL2-S target cells
Page 1 and 2: Development of a Bioluminescent Cel
Page 3 and 4: Signaling pathways activated in eff
Page 5: Protocol 1. Reference or test antib
Page 9 and 10: Bioassay development: Optimizing Ef
Page 11 and 12: Bioassay development: Evaluating cl
Page 13 and 14: Target cells can also be frozen, th
Page 15 and 16: ADCC Reporter Bioassay using frozen
Page 17 and 18: Precision, accuracy and linearity o
Page 19 and 20: ADCC Reporter Bioassay is stability
Page 21 and 22: Additional target cell option: Raji
Page 23 and 24: iological activity of therapeutic a
Page 25 and 26: Planned ADCC Reporter Bioassay kit
Page 27: For more information Confidential a

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