Development of a Bioluminescent Cell-Based Assay to ... - Promega
Development of a Bioluminescent Cell-Based Assay to ... - Promega
Development of a Bioluminescent Cell-Based Assay to ... - Promega
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Pro<strong>to</strong>col<br />
1. Reference or test antibody is<br />
added <strong>to</strong> target cells.<br />
2. Effec<strong>to</strong>r cells are added and<br />
response is induced in as little as<br />
6 hours. Effec<strong>to</strong>r cells consist <strong>of</strong><br />
a Jurkat cell line engineered <strong>to</strong><br />
express FcgRIIIa (V158) recep<strong>to</strong>r<br />
and NFAT-RE luc2 luciferase.<br />
3. Luciferase detection reagent is<br />
added and luminescence is<br />
measured immediately.<br />
Confidential and Proprietary. Not for Further Disclosure.
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