Development of a Bioluminescent Cell-Based Assay to ... - Promega
Development of a Bioluminescent Cell-Based Assay to ... - Promega
Development of a Bioluminescent Cell-Based Assay to ... - Promega
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Precision, accuracy and linearity <strong>of</strong><br />
ADCC Reporter Bioassay<br />
Antibody Measured Mean<br />
RSD<br />
Sample Potency (%) Potency (%) SD % Recovery (%) (%)<br />
day 1 48.5<br />
day 2 50% 45.2 48.9 3.9 97.7 7.9<br />
day 3 52.9<br />
day 1 63.1<br />
day 2 75% 62.9 66.4 5.9 88.5 8.9<br />
day 3 73.2<br />
day 1 112.1<br />
day 2 125% 136.3 123.0 12.3 98.4 10.0<br />
day 3 120.5<br />
day 1 148.8<br />
day 2 150% 150.4 147.6 3.6 98.4 2.4<br />
day 3 143.6<br />
Confidential and Proprietary. Not for Further Disclosure.<br />
Linearity<br />
y= 1.016x – 0.052<br />
R 2 = 0.995<br />
<strong>Assay</strong> with frozen, thaw-and-use<br />
effec<strong>to</strong>r and WIL2-S target cells<br />
Observed potency<br />
175%<br />
150%<br />
125%<br />
100%<br />
75%<br />
50%<br />
25%<br />
0%<br />
Precision<br />
(Av <strong>of</strong> RSD(%))<br />
7.3%<br />
y = 1.0161x - 0.0516<br />
R² = 0.9949<br />
0% 25% 50% 75% 100% 125% 150% 175%<br />
Expected potency<br />
Accuracy<br />
(Av <strong>of</strong> % Recovery)<br />
95.8%<br />
17
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