PrecisION TM hERG-HEK - Nanion Technologies Gmbh

NPC - certified cells
Nanion NPC-certified cells.
The optimum choice for your assays.
PrecisION TM hERG-HEK
Cells from Merck Millipore, optimized for Nanion Patch Clamp Devices
(NPC) Port-a-Patch, Patchliner and SyncroPatch96.
• Assay optimized for Nanion APCs
• Giga Ohm seals
• High success rates
• Stable current responses
• Cell line and assay support
• Optimized patch clamp solutions
NPC - certified cells
See www.nanion.de for more Nanion approved cells.

Nanion Europe
info@nanion.de
phone +49 89 218997972
www.nanion.de
Nanion NPC-certified hERG-HEK cells
The hERG gene (KCNH2) encodes a K + ion channel
responsible for the repolarizing IKr current in the cardiac
action potential. Given the importance of this channel in
maintaining cardiac function, it has become an important
target in compound safety screening.
Compounds can display different properties or different
potencies at physiological temperature (35°C) vs. room
temperature (RT). Therefore, it is of advantage to be able
to study this channel electrophysiologically at elevated
temperature as shown below.
A B
Control
100 µM
Erythromycin
200 pA
200 ms
A hERG inhibition of Erythromycin at 35°C as recorded in hERG-HEK cells. B CRCs for
erythromycin at room temperature and at 35°C. Erythromycin is approximately 10-fold
more potent at 35°C compared with RT. Recordings were done on a Patchliner.
hERG-HEK from Merck Millipore
Nanion Technologies GmbH
Erzgiessereistr. 4
80335 Munich, Germany
Nanion USA
info@naniontech.com
phone 1-888-9-NANION
www.naniontech.com
Inhibition
Passage stability: > 30
Nanion China
info@nanion.cn
phone +86 1082176386
www.nanion.cn
Current amplitude / cell: 919 ± 57 pA (n=54)
phone +49 89 218997972
fax +49 89 218997960
www.nanion.de • info@nanion.de
Raw data traces and CRCs as obtained from hERG expressing hERG-HEK cells.
IC 50 s: Astemizole, IC 50 =1.5 nM (n=34); Propafenone
IC 50 =270 nM (n=15); Quinidine IC 50 =408 nM
(n=16); Terfenadine IC 50 =9.1 nM (n=25); Cisapride
IC 50 =38.5 nM (n=23); E4031 IC 50 =45.5
nM (n=6); Flunarizine, IC 50 =163.7 ± 19 nM (n=3)
Erythromycin IC 50 @ RT= 427.5 µM (n=12),
Erythromycin IC 50 @ 35°C = 30.7 µM (n=18);
Seal resistance: 1.3 ± 0.7 GΩ
CSlow: 7.0 ± 1 pF (n=16)
Rs: 7.5 ± 1 (n=16)
Cell stability after harvesting: ~ 4 hrs
Average whole cell stability: ~ 45 min
Successful whole cell recordings: 70 - 90 %
RT
35ºC
Application directly from frozen stock: yes
1.0
0.8
0.6
0.4
0.2
0.0
0.1 1 10 100 1000
Conc Erythromycin (µM)
Astemizole
3nM Quinidine
300nM
1µM
3µM
10µM
250 ms 250 ms
Propafenone
NPC-certified cells. The optimum choice.
100 pA
inhibition
1
250 ms
IC 50 = 1.5 ± 0.3 nM
(n=34)
10pM
1nM
3nM
10nM
30nM
0
0.0010.01 0.1 1 10 100 1000
Astemizole (nM)
Terfenadine
250 pA
inhibition
1
30pM
3nM
10nM
30nM
100nM
200 pA
inhibition
1
IC 50 = 270 ± 35 nM
(n=15)
0
0.0010.01 0.1 1 10 100
Propafenone (nM)
Cisapride
100pM
10nM
30nM
100nM
300nM
500 pA
inhibition
1
IC 50 = 408 ± 45 nM
(n=16)
3nM
300nM
1µM
3µM
10µM
0
0.0010.01 0.1 1 10 100
Quinidine (nM)
E4031
250 ms 250 ms 250 ms
IC 50 9.1 ± 1.8 nM
(n=25)
250 pA
inhibition
1
IC 50 = 38.5 ± 9 nM
(n= 23)
100pM
10nM
30nM
100nM
300nM
0
0
0
0.01 0.1 1 10 100 1000
Terfenadine (nM)
0.1 1 10 100 100010000
Cisapride (nM)
0.1 1 10 100 100010000
E4031 (nM)
100 pA
inhibition
1
IC 50 = 45.5 ± 7 nM
(n= 6)