49th Symposium

49 th Symposium of the Society for Histochemistry 

Freiburg, im Breisgau, Germany, 26. – 29. September 2007 

49th Symposium 

of the 

Society for Histochemistry 

A platform for talents


Freiburg im Breisgau, Germany, 26. - 29. September 2007 

GENERAL INFORMATION 

Location: 

Foto: Guido Kirsch, Freiburg 

Institute of Pathology, 

University Hospital Freiburg, 

Breisacherstr. 115a 

79106 Freiburg 

Germany 

Organizers: 

Martin Werner and Christoph Peters 

Organizing-Committee: 

Manuela Haller and Silke Lassmann 

Scientific Committee: 

Peter Friedl, Heinz Höfler, Axel zurHausen, Silke Lassmann, 

Roland Nitschke, Oliver Opitz, Christoph Peters, Thomas Reinheckel, 

Martin Werner

13:30-15:30 



Wednesday, 26 September 2007 

12:00 Registration desk open 

Workshop I: Microdissection and DNA/RNA/Protein Analyses (Leica) 

Chairs: Henry Dijkman, Bart Smeets (Nijmegen, NL) 

13:30-13:50 “Laser micro dissection - Material preparation and upstream processing”, Gerhard 

Zimmermann (Leica) 

13:50-14:20 „Laser micro dissection - system, applications and analysis“, Gerhard Zimmermann (Leica) 

14:20-15:00 “Laser micro dissection - Material preparation and downstream applications”, Henry Dijkman, 

Bart Smeets, Nijmegen, NL 

15:00-15:30 Hands on session 

15:30-16:00 Break 

16.00-18.00 

Workshop II DNA/RNA/Microarrays (Affymetrix) 

Chairs: Matthias Prucha (Affymetrix, UK), Paul Fisch (Freiburg, GER) 

16:00-16:20 „To be announced“, Hendrik Veelken, Freiburg, GER 

16:20-16:40 „Genome-wide analysis of genetic alterations in Barrett’s adenocarcinoma using single 

nucleotide polymorphism arrays“, Thorsten Wiech, Freiburg, GER 

16:40-17:20 „Recent progress using DNA and RNA extracted from formalin-fixed, paraffin-embedded 

(FFPE) samples for Microarrays in order to relate Gene Expression profiles with patterns of 

Copy Number changes and LOH“, Matthias Prucha 

17:20-18:00 „To be announced“, Richard Pallin 

18:15-18:45 Symposium Opening 

18:45-19.30 Robert Feulgen Lecture 

“New ways of looking at synapses”, Michael Frotscher, Freiburg, GER 

19:45 Welcome Party



Thursday, 27 September 2007 

8:30-10:00 Session I (Part 1): Light-Microscopic Molecular Detection 

8:30-8:45 

Chairs: Ronald Simon (Hamburg, GER), Luigi Terracciano (Basel, CH) 

1 “Putting Proteins into Perspective: Ultra high content imaging in the light of the cellular 

toponome”, Claus Wittpoth, Magdeburg, GER 

8:45-9:00 2 “Tracing Skin and Blood Topoproteomics in Psoriasis by Multi Epitope Ligand 

Cartography Robot Technology (MELC RobTec)”, Raik Böckelmann, Magdeburg, GER 

9:00-9:15 3 “COMBO - FISH and SMI - Microscopy: novel techniques for gene 

compaction measurements”, Michael Hausmann, Heidelberg, GER 

9:15-9:30 4 “Low-level copy gains occur frequently in Barrett’s cancer and define a biologically 

significant subgroup”, Daniel Veith, Munich GER (Abstract) 

9:30-9:45 5 “Pituitary tumor transforming gene 1: a new histopathological biomarker for primary 

cholongiocarcinomas”, Stefano Musardo, Milan, Italy 

9:45-10:00 6 “Does size matter? TMAs are a valuable tool to simulate prostate biopsies”, Olaf J.C. Hellwinkel, 

Hamburg, GER 

10:00-10:30 Break / Posters / Exhibition 

10:30-12:00 Session I (Part 2): Light-Microscopic Molecular Detection 

Chairs: Luigi Terracciano (Basel, CH), Ronald Simon (Hamburg, GER, 

10:30-10:50 1 “Novel applications of ROC curves to the immunohistochemical analysis of protein 

markers in colorectal cancer”, Inti Zlobec, Basel, CH 

10:50-11:10 2 “Role of metastasis suppressor gene Raf-1 kinase inhibitor protein (RKIP) in colorectal 

cancer stratified by mismatch repair status“, Alessandro Lugli, Basel, CH 

11:10-11:30 3 “Regulation of alpha-smooth muscle actin protein expression in fetal growth restriction 

preclamptic placentas”, Mario Castellucci, Ancona, Italy 

11:30-11:45 4 „Luminescent Conjugated Polymers: A Promising Tool in Diagnosis of Systemic 

Amyloidosis“, Kristian Ickenberg, Zurich, CH 

11:45-12:00 5 “Expression of glycodelin A in normal pregnancy and hydatidiform mole”, Bettina Toth, 

Munich, GER 

12:00-14:00 LUNCH (SAKURA) / Exhibition 

12:00-14:00 Workshop III: Tissue Arrays (Leica) 

Chair: Ronald Simon (Hamburg, GER) 

12:00-12:45 “Tissue microarray applications: scale, quality, and automation”, Ronald Simonn, Hamburg, GER 

12:45-13.30 “TissueFAXS – Microscope based cell analysis system for cells, tissues, smears and TMA’s”, Katja 

Österreicher/Rupert Ecker, TissueGnostics GmbH, Vienna, Austria 

13:30-14:00 Practical Session and Discussion



14:00-15:30 Session II (Part 1): Functional Micro-Architecture of Cells and Tissue 

Chairs: Thomas Reinheckel (Freiburg, GER), Galia Blum (Stanford, USA) 

14:00-14:30 1 Keynote Lecture: 

14:30-14:45 

“Optical imaging of cysteine protease activity in cells and living subjects using quenched 

fluorescent activity based probes (qABPs)”, Galia Blum, Stanford, USA 

2 “Perichromatin fibrils as early markers of transcriptional alterations”, Marco Biggiogera, 

Pavia, Italy 

14:45-15:00 3 “In vitro model for age-related macular degeneration (AMD) – Quantification of the 

angiogenic potential of normal and diseased human retinal pigment epithelium”, Andreas 

Stahl, Freiburg, GER 

15:00-15:15 4 “Costameric localization of the TRPC3 cation channel in isolated mouse skeletal muscle 

fibres”, Christiane Kunert-Keil, Greifswald, GER 

15:15-15:30 5 “Cancer cell invasion causes stressful nuclear deformation and genomic instability”, 

Stephanie Alexander, Würzburg, GER 

15:30-16.30 Break / Posters / Exhibition 

16:30-18:00 Session II (Part 2): Ultrastructural detection of molecular targets 

Chairs: Pavel Hozak (Prague, CZ), Paul Verkade (Bristol, UK) 


“High time resolution Correlative Light Electron Microscopy using High-Pressure Freezing”, 

Paul Verkade, Bristol, UK 

15:00-17:15 2 “Methods for sensitive immunogold labelling on ultrastructurally preserved biological 

samples: optimised cryro-methods and spatial statistics”, Pavel Hozak, Prague, CZ 

17:15-17:30 3 “Protection against oxidative stress and ketogenesis in fatty liver grafts preserved by 

machine perfusion at 20°C respect to cold storage”, Eleonora Boncompagni, Pavia, Italy 

17.30-17:45 4 “DAB-photoconversion in combination with 3D electron tomography for studies using 

correlative microscopy”, Josef Neumüller, Vienna, Austria 

17:45-18:00 5 “Nuclear orientation of transcribed BCL2 in non-neoplastic and neoplastic cervical 

squamous epithelium”, Thorsten Wiech, Freiburg,GER 

18:15 Lecture of Robert Feulgen-Prize Winner 

“Nuclear compartmentalization in interphase mammalian nuclei by 

histochemical methods”, Ana Pombo, London, UK



Friday, 28 September 2007 

8:30-10:00 Session III: Molecular Tracing in Living Cells 

Chairs: Peter Friedl (Würzburg, GER), Roland Nitschke (Freiburg, GER) 

8:30-9:00 1 Keynote Lecture („Zeiss-Lecture“): 

„To be announced“, Robert F. Murphy, Pittsburg, USA 

9:00-9:30 2 “Force generation during interstitial leukocyte chemotaxis” Michael Sixt, Martinsried, GER 

9:30-10:00 3 „Tumor invasion in 5 dimensions”, Peter Friedl, Würzburg, GER 


10:30-12:00 Session IV (Part 1): Molecular Methods on Pathological Specimens 

Chairs: Holger Moch (Zurich, CH), Martin Werner (Freiburg, GER) 

10:30-10:45 1 “Immunohistochemical triple stains in the analysis of extrafollicular B-cell activation”, 

Thomas Rüdiger, Karlsruhe, GER 

10:45-11:00 2 “The detection of Helicobacter bacteria in biopsies by molecular methods”, Gieri 

Cathomas, Liestal, CH 

11:00-11:15 3 “A simple histochemical assay to detect cancer cells”, Cornelis JF Van Noorden, 

Amsterdam, NL 

11:15-11:30 4 “Oligonucleotide microarray analysis of the global gene expression of paraffin-embedded 

tumor samples“, Matthias Frank, Frankfurt, GER 

11:30-11:45 5 “ A novel Biochip platform focussing on clinical requirements”, Thomas Brandstetter, 

Freiburg, GER 

11:45-12:00 6 „The Heidelberg peptide nanocomputer: complex, combinatorial peptide 

libraries on semiconductor computer chips“, Michael Hausmann, Heidelberg, GER 

12.00-14.00 LUNCH (SAKURA) / Exhibition 

12:00-14:00 Workshop IV: Live Microscopy (Leica) 

Chair: Peter Friedl (Würzburg, GER) 

14:00-14:40 “Innovations in confocal and life cell imaging technologies (TIRF, FRET, FLIM, FRAP, 

STED etc.)”, Karl-Heinz Körtje (Leica) 

14:40-15:20 “Multimodal confocal microscopy to visualize pericellular proteolysis at the cell-matrix 

interphase”, Peter Friedl, Würzburg, GER 

15:20-16:00 Live demonstration




Chairs: Heinz Höfler, Marius Üffing (Munich, GER) 

1 „To be announced“, Axel Walch, Munich, GE 

2 To be announced 






Chairs: Heinz Höfler, Marius Üffing (Munich, GER) 

1 “Making it small: Protein Microarrays – Applications and future challenges“, Thomas 

Joos, Tübingen, GER 





18:15-19:15 Society Meeting 

20:00 - Congress Dinner: 

Ringhotel „Zum Roten Bären“, Oberlinden 12, Freiburg



Saturday 29 September 2007 

9:00-10:30 Session V (Part 1): Tumors – Carcinogenesis, Progression 

Chairs: Oliver Opitz, Silke Lassmann (Freiburg, GER) 

9:00-9:30 1 Keynote Lecture: "Malignant progression in colorectal cancer: EMT, ß-Catenin & 

cancer stem cells" Thomas Brabletz 

9:30-9:45 2 “Interaction between tumor and environment: TGF-ß R2 downregulation in tumourassociated 

stroma worsens prognosis and high-grade tumours show more tumourassociated 

macrophages and lower TGF-ß1 expression in colon carcinoma”, Arno 

Dimmler, Karlsruhe, GER 

9:45-10:00 3 “Induction of syncytium formation in trophoblast tumour cells BeWO by galectin-1”, Udo 

Jeschke, Munich, GER 

10:00-10:15 4 “Cathepsins B and X promote tumor development and lung metastasis in a murine 

breast cancer model”, Uta Schurigt, Freiburg, GER 

10:15-10:30 5 “ EBV-infection in cardiac and non-cardiac gastric carcinoma is associated with 

aberrant methylation of p16, p14 and APC, but not with hMLH1“, Helene Geddert, 

Karlsruhe, GER 


11:00-12:30 Session V (Part 2): Tumors - Predictive Markers 

Chairs: Axel zur Hausen (Freiburg, GER), Joost Oudejans (Amsterdam, 

NL) 


“To be announced”, Joost Oudejans, Amsterdam, NL 

11:30-11:45 2 „Reliability of slide-based steroid receptor analysis and HER-2 testing: results and 

conclusions of the national quality assurance program „Quip“, Reinhard von Wasielewski, 

Hannover 

11:45-12:00 3 “Microarray based prediction of tumor response to neoadjuvant chemoradiotherapy of 

patients with locally advanced rectal cancer”, Robert Rosenberg, Munich, GER 

12:00-12:15 5 “Free peritoneal tumour cells are an independent prognostic factor even in curatively 

resected gastric carcinoma UICC stage I”, Robert Rosenberg, Munich, GER 

12:15-12:30 6 „Prognostic significance of Trransketolase L1 in malignant tumours“, Gian Kayser, 

Freiburg, GER 

12:30 Closure of the Symposium 

12:45-14:45 Lunch, departure



ACKNOWLEDGEMENTS 

It would not have been possible to organize the symposium without the 

support of the following institutions and companies: 

Main Sponsor: 

Leica Microsystems 

Sponsors (16.8.07): 

Affymetrix 

Bruker Daltonics 

DAKOCytomation 

MetaSystems 

Sakura 

Carl Roth 

Olympus 

ThermoElectron 

Supporting Institutions: 

Tumorzentrum Ludwig-Heilmeyer, Comprehensive Cancer Center Freiburg 

Medical Faculty Freiburg, University Hospital Freiburg 

Albert-Ludwigs University Freiburg 

kongress und kommunikation gGmbH, Freiburg 

Fit - Freiburger Incoming Touristik, Freiburg

49th Symposium

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?