Target Discovery and Validation Reviews and Protocols

Gene Networks 39
Fig. 4. Downstream pathway of the virtual gene YEXP100mg30min. The shadowed
genes are affected by the drug (11).
Finally, by considering the dag whose root is the virtual gene, the children of
this virtual gene would be the candidate genes directly affected by the drug.
Note 2 gives more comments.
3.1.2. Application of Virtual Gene Technique
Figure 4 is the result of the virtual gene technique for the drug corresponding
to “100mg and 30min.” The root node is the virtual gene corresponding
to the drug, and the genes in the shadowed area are the candidates of the drugaffected
genes. By applying the virtual gene technique to each drug response
data and disruptant microarray data, we can obtain the candidates of the drugaffected
genes. Figure 5 is the superimposed graph of virtual gene technique
generated for five separate time and dosage griseofulvin exposure experiments.
Figure 5 suggests that the CIK1 (Gene Ontology [GO] annotation
[function]:microtubule motor activity) expression is significantly affected
under exposure to griseofulvin at each dosage and time point and can be
viewed as a drug-affected genes with high reliability (Notes 3 and 4).