Target Discovery and Validation Reviews and Protocols

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Keratin Transgenics and Knockouts 251 117. Gu, H., Marth, J. D., Orban, P. C., Mossmann, H., and Rajewsky, K. (1994) Deletion of a DNA polymerase beta gene segment in T cells using cell typespecific gene targeting. Science 265, 103–106. 118. Guo, C., Yang, W., and Lobe, C. G. (2002) A Cre recombinase transgene with mosaic, widespread tamoxifen-inducible action. Genesis 32, 8–18. 119. Utomo, A. R., Nikitin, A. Y., and Lee, W. H. (1999) Temporal, spatial, and cell type-specific control of Cre-mediated DNA recombination in transgenic mice. Nat. Biotechnol. 17, 1091–1096. 120. Wunderlich, F. T., Wildner, H., Rajewsky, K., and Edenhofer, F. (2001) New variants of inducible Cre recombinase: a novel mutant of Cre-PR fusion protein exhibits enhanced sensitivity and an expanded range of inducibility. Nucleic Acids Res. 29, E47. 121. Schaft, J., Ashery-Padan, R., van der Hoeven, F., Gruss, P., and Stewart, A. F. (2001) Efficient FLP recombination in mouse ES cells and oocytes. Genesis 31, 6–10. 122. Denk, H., Lackinger, E., Zatloukal, K., and Franke, W. W. (1987) Turnover of cytokeratin polypeptides in mouse hepatocytes. Exp. Cell Res. 173, 137–143. 123. Yu, Y. and Bradley, A. (2001) Engineering chromosomal rearrangements in mice. Nat. Rev. Genet. 2, 780–790. 124. Eggan, K., Akutsu, H., Loring, J., et al. (2001) Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. Proc. Natl. Acad. Sci. USA 98, 6209–6214. 125. Eggan, K., Rode, A., Jentsch, I., et al. (2002) Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation. Nat. Biotechnol. 20, 455–459. 126. Seibler, J., Zevnik, B., Kuter-Luks, B., et al. (2003) Rapid generation of inducible mouse mutants. Nucleic Acids Res. 31, e12. 127. Nagy, A. G. M., Vintersten, K., and Behringer, R. (2003) Manipulating the Mouse Embryo: A Laboratory Manual, 3rd, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. 128. Magin, T. M., McEwan, C., Milne, M., Pow, A. M., Selfridge, J., and Melton, D. W. (1992a) A position- and orientation-dependent element in the first intron is required for expression of the mouse hprt gene in embryonic stem cells. Gene 122, 289–296. 129. Magin, T. M., McWhir, J., and Melton, D. W. (1992b) A new mouse embryonic stem cell line with good germ line contribution and gene targeting frequency. Nucleic Acids Res. 20, 3795–3796. 130. Ying, Q. L., Nichols, J., Chambers, I., and Smith, A. (2003) BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 115, 281–292. 131. Ying, Q. L. and Smith, A. G. (2003) Defined conditions for neural commitment and differentiation. Methods Enzymol. 365, 327–341. 132. Kupriyanov, S. and Baribault, H. (1998) Genetic control of extraembryonic cell lineages studied with tetraploiddiploid chimeric concepti. Biochem. Cell Biol. 76, 1017–1027.
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METHODS IN MOLECULAR BIOLOGY 360 T
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M E T H O D S I N M O L E C U L A R
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© 2007 Humana Press Inc. 999 River
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vi Preface get. Approaches to targe
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viii Contents 10 Transgenic Animal
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x Contributors SAHOHIME MATSUMOTO
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xii Contents of Volume 2 12 Validat
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2 Sioud disease pathogenesis and pe
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4 Sioud A more fruitful approach fo
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6 Sioud both transient and permanen
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8 Sioud serum biomarkers. This syne
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10 Sioud Fig. 2. Schematic of targe
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12 Sioud 13. Magin, T. M. (1998) Le
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Harnessing the Human Genome 15 The
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Harnessing the Human Genome 17 Fig.
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Harnessing the Human Genome 23 The
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Harnessing the Human Genome 27 repr
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Harnessing the Human Genome 29 14.
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Harnessing the Human Genome 31 45.
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34 Imoto et al. (4,5), and Bayesian
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36 Imoto et al. 2. Materials Two ty
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38 Imoto et al. Fig. 3. Graphical v
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40 Imoto et al. Fig. 5. Result of t
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42 Imoto et al. p(D |G) = ∫ p(D,
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44 Imoto et al. β k (k = 1,…,q j
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46 Imoto et al. Fig. 7. Partial res
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48 Imoto et al. Fig. 8. Strategy to
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50 Imoto et al. Fig. 9. (continued)
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52 Imoto et al. Table 3 List of Roo
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54 Imoto et al. 5. De Hoon, M. J. L
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56 Imoto et al. 39. Kamimura, T., S
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58 Beaty et al. cytotoxic drugs and
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60 Beaty et al. Fig. 1. Principles
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62 Beaty et al. oligonucleotide mic
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64 Beaty et al. 3. The 12% polyacry
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66 Beaty et al. 2.3.3. Protein Stai
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68 Beaty et al. 3.1.3. Labeling of
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70 Beaty et al. 1 µL of the anneal
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72 Beaty et al. 4. Phenol:cholorfor
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74 Beaty et al. 19. Wash twice with
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76 Beaty et al. The Following Volum
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78 Beaty et al. using a protein ass
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80 Beaty et al. 6. Shake the gel fo
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82 Beaty et al. Fig. 2. Preparation
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84 Beaty et al. search in. A widely
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86 Beaty et al. 3. Kern, S. E., Hru
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88 Beaty et al. 34. Peters, D. G.,
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5 Molecular Classification of Breas
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Molecular Profiling of Breast Cance
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Fig. 2. (Continued) the right ident
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6 Discovery of Differentially Expre
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Gene Target Discovery 117 In the fi
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Gene Target Discovery 119 sequences
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Gene Target Discovery 121 There is
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Gene Target Discovery 123 digestion
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Gene Target Discovery 125 Fig. 1. P
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Gene Target Discovery 127 5. Sargen
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Gene Target Discovery 129 41. Berry
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132 Matsumoto, Miyagishi, and Taira
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144 Fig. 1. The ribozyme-expression
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146 Sano and Taira 5. 10X L (low-sa
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148 Sano and Taira ribozymes may cl
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150 Fig. 3. A system for the identi
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152 Sano and Taira 4. Notes 1. We r
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9 Production of siRNA- and cDNA-Tra
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Transfected Cell Arrays 157 Fig. 1.
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Transfected Cell Arrays 159 2. The
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Transfected Cell Arrays 161 5. Simp
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164 Houdebine Fig. 1. Different met
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166 Houdebine concentrations become
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168 Houdebine Fig. 2. Major methods
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170 Houdebine integrate into the me
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172 Houdebine 2.2.3. Knock-In Into
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174 Houdebine as insulators. The co
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176 Houdebine Fig. 5. Different met
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178 Houdebine systems. Moreover, st
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180 Houdebine contribute to generat
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182 Houdebine Fig.7. Example of a v
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184 Houdebine more extensively. Tra
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186 Houdebine and stroma. Several o
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188 Houdebine explained at the mole
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190 Houdebine of the intestinal epi
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192 Houdebine interference of the g
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194 Houdebine 17. Whitelaw, C. B. A
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196 Houdebine 51. Bell, A. C., West
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198 Houdebine 86. Carmichael, G. G.
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Page 217 and 218: 204 Vijayaraj, Söhl, and Magin 1.
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Page 255 and 256: 242 Table 3 Time Schedule For Aggre
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302 Sioud (see Chapter 15, Volume 1
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304 Sioud display if the foreign pr
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306 Sioud Fig. 13. Immunoscreening
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308 Sioud molecular techniques have
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310 Sioud Although much of the init
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312 Sioud Because of their importan
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314 Sioud 22. He, X., Janeway, C. A
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316 Sioud 59. Sahin, U., Tureci, O.
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318 Sioud 91. Golgher, D., Jones, E
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320 Jäger Furthermore, this immune
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322 Jäger Antigens that are tumor-
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324 Jäger 2. Nakano, O., Sato, M.,
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326 Jäger 31. Chen, Y. T., Gure, A
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328 Le Naour dependent on CD8+ cyto
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330 Le Naour Fig. 1. To illustrate
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332 Le Naour heterogeneity of HCC,
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334 Le Naour 15. Le Naour, F., Mise
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336 Cekaite, Hovig, and Sioud Yeast
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338 Cekaite, Hovig, and Sioud diffe
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340
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342 Cekaite, Hovig, and Sioud nonra
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344 Cekaite, Hovig, and Sioud 3. Ue
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346 Cekaite, Hovig, and Sioud 32. T
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348 Cekaite, Hovig, and Sioud 65. L
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350 Index Centroblast, 289 Centrocy
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352 Index L Labeling mRNA, 62 Large
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354 Index Tetraploid embryos, 239 g
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