Target Discovery and Validation Reviews and Protocols

106 Sørlie
Fig. 5. Cluster of genes associated with TP53 mutational status. Hierarchical clustering
of 158 genes whose expression levels were significantly correlated to the mutation
status of the TP53 gene (SAM, false discovery rate < 1). Only tumor tissues from the
two prospective studies from Norway were included in the cluster analysis. The tumor
samples are ordered according to the clustering groups from Fig. 3. Frequencies of
TP53 mutations are listed. Adapted from ref. 67.
rate of less than 1% (67). The genes were clustered, whereas the samples were
ordered according to the subtypes (Fig. 5). As expected, many of the genes that
were highly expressed in the mutated tumors (mostly basal-like and luminal B
type tumors) are cell cycle regulated genes such as PLK1, CCNA2, STK6, and
MAPK13 (17). Two of the genes coexpressed with ERBB2 oncogene, GRB7 and
STARD3, also were found in this gene list, but not the ERBB2 gene itself.
Interestingly, many of the genes whose high expression and specific pattern