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JST Vol. 21 (1) Jan. 2013 - Pertanika Journal - Universiti Putra ...

JST Vol. 21 (1) Jan. 2013 - Pertanika Journal - Universiti Putra ...

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ISSN: 0128-7680 © <strong>2013</strong> <strong>Universiti</strong> <strong>Putra</strong> Malaysia Press.<br />

<strong>Pertanika</strong> J. Sci. & Technol. <strong>21</strong> (1): 29 - 36 (<strong>2013</strong>)<br />

SCIENCE & TECHNOLOGY<br />

<strong>Journal</strong> homepage: http://www.pertanika.upm.edu.my/<br />

Different Media Formulation on Biocellulose Production by<br />

Acetobacter xylinum (0416)<br />

Suryani Kamarudin 1,2 *, Mohd Sahaid, K. 1 , Mohd Sobri, T. 1 , Wan Mohtar, W. Y. 3 ,<br />

Dayang Radiah, A. B. 2 and Norhasliza, H. 2<br />

1 Department of Chemical and Process Engineering, Faculty of Engineering and Build Environment,<br />

University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia<br />

2 Department of Chemical and Environmental Engineering, Faculty of Engineering, University <strong>Putra</strong><br />

Malaysia, 43400 Serdang, Selangor, Malaysia<br />

3 Department of Bioscience and Biotechnology, Faculty of Science, University Kebangsaan Malaysia, 43600<br />

Bangi, Selangor, Malaysia<br />

ABSTRACT<br />

Biocellulose (BC), produced by Acetobacter xylinum (0416), was carried out using three types of medium<br />

composition under static surface culture. The media used in this experiment included CWHSM (Coconut<br />

water in Hestrin-Schramm medium), CM (Complex medium) and HSM (Hestrin-Schramm medium).<br />

CWHSM and CM used coconut water from agro-waste as the main source of sugar. The fermentation<br />

was conducted for 12 days and the results of BC dry weight, cell entrapped, pH medium and productivity<br />

were evaluated and compared. The results show that CWHSM is the most suitable medium for BC<br />

production with a productivity of up to 0.044 g l -1 day -1 .<br />

Keywords: Acetobacter xylinum, agro-waste, biocellulose, coconut water, production medium<br />

Article history:<br />

Received: 1 February 2011<br />

Accepted: 4 October 2011<br />

E-mail addresses:<br />

suryani@eng.upm.edu.my (Suryani Kamarudin),<br />

sahaid@eng.ukm.my (Mohd Sahaid, K.),<br />

sobri@vlsi.eng.ukm.my (Mohd Sobri, T.),<br />

wantar@pkrisc.cc.ukm.my (Wan Mohtar, W. Y.),<br />

dayang@eng.upm.edu.my (Dayang Radiah, A. B.),<br />

snowiequeen809@yahoo.com (Norhasliza, H.)<br />

*Corresponding Author<br />

INTRODUCTION<br />

It is known that some Acetobacter strains<br />

produce cellulose. This cellulose is called<br />

bacterial cellulose (BC) (Ross et al., 1991).<br />

BC is a chemically pure form of cellulose<br />

and it is free from hemicellulose, pectin,<br />

and lignin, which are associated with plant<br />

cellulose and are difficult to eliminate<br />

(Bielecki et al., 2002; Jung et al., 2005).<br />

In addition, BC is extremely pure and it<br />

exhibits a high degree of polymerisation<br />

and crystallinity. BC has many applications

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