Sangrovit - Jadis Additiva

Importance of bitter substances originating from
Sangrovit as modulator between animal health and
performance
Dr. Kathrin Tschirner; Phytobiotics Feed Additives; Eltville; Germany
Rennes, 11.06.2009

Sangrovit ® - An unique Product with approved Active Substances
The Product
Standardized, phytogenic feed additive
Manufactured from one selected plant
(Papaveraceae family)
Defined, analysable active substances
Scientific based, proven efficiency
Documented Safety
Transparent Production

Sangrovit ® - An unique Product with approved Active Substances

Sangrovit ® - An unique Product with approved Active Substances
Active Principles
Produced by the plant as defence substances
(phytoalexines)
Chemical classification: Benzophenanthridine alkaloids (BA)
Functional classification: Bitter substances
BA possess widespread biological activities
Used in human/veterinary phytopreparations and dental care products since a long time
Phytobiotics’ research shows that BA can impact animal performance and health
Secondary plant metabolites

Bitter substances
Definition: No defined chemical group, classification occurs in accordance to its function
and taste
MOA: Bitters bind to T2R-receptors (located on the tong ground) → release of calcium →
release of neuro-transmitters → activation of the Nervus Vagus
(Bitter taste receptors expressed mainly in the mouth, but also in the gastrointestinal
tract 1 )
Effects: stimulate digestive enzymes, gastric and pancreatic juice, increase appetite, have
a general tonic action on digestion
Bitter taste receptors react to very low concentrations
(protection)
1 S. V. Wu et al. (2002)

Sangrovit ® - Unique, known Mode of Action
Stimulation of various digestive organs and functions
Improved absorption of nutrients
Improved liver function and health
Anti-inflammatory properties
Inhibition of enzymatic degradation of amino acids
Higher growth performance (FI, FCR, DWG)
Improved carcass quality
Health promoting/stabilizing properties (e.g. lower
mortality)

Sangrovit ® - Unique, known Mode of Action
Improved liver
health
Stimulation
digestion
Sangrovit
Anti-inflammatory
effects
Amino Acids
AADE
Healthier Mucosa
Better Absorption
Higher
Carcass value
Stabilization of Immune-System
Improved
N-Balance
Higher amino acid levels in the blood
Higher availability of amino acids (e.g Trp)
Amino Acids ⇑
Nutrients for bacteria ⇓
Indol, Skatol ⇓
Less harmful substances
at mucosa surface

Higher Availability of Amino Acids
Plasma Lys Lys Concentration Concentration (µmol/l) (µmol/l)
(µmol/l)
600
500
400
300
200
100
0
a
a,b = significant differences
Effect of Sangrovit Amino Acid Concentration in the Blood
Lysine
b
Control Sangrovit
Institute of animal nutrition, University Kiel, Germany (2003)
Plasma Plasma Try Try Try Concentration Concentration (µmol/l) (µmol/l) (µmol/l)
70 70
60 60
50 50
40 40
30 30
20
10
0
a
Tryptophan
b
Control Sangrovit

Higher Availability of Amino Acids - Impact
Optimized AS metabolism
Improved N-balance
Lees urea in the blood → less detoxfication for the liver
Improved N/Protein retention
Better carcass quality and value
…Special Impact of TRP
Amino acid for protein metabolism
Precursor for serotonin metabolism (►In the intestine, serotonin influences the
secretion of digestive enzymes and the motility of the gut (Blundell, 1992)).
Positive influence on protein metabolism in the liver (Peters, 1991)
Supports liver health and liver metabolism in general
Supports the immune system

Higher Availability of Amino Acids - Impact
Sangrovit ® Improves N-Balance
(Research Station HOECHST)
Control Flavomycine Sangrovit
Weight gain, g/d 796 822 821
FCR 2.24 2.20 2.16
N excretion 100 94 91
N retention 100 107 108
N balance
Urea blood
100
100
106
91
109
Ammonia blood 100 102 92
Improved N-Retention and Balance
Less Urea and Ammonia in the blood
76
Sangrovit ® Influences Serum Urea in lactating
sows (University of Kiel, 2003)
Urea Serum Serum concentration
(mg/dl) (mg/dl)
35
30
25
20
15
10
5
***
Control Sangrovit
30 ppm
Sangrovit ® = Less Urea and Ammonia in the
blood

Higher Availability of Amino Acids - Impact
Effect of Sangrovit on carcass quality in pigs
Institute of animal nutrition, University Kaunas, Lithuania (2003)
120
%
110
100
90
80
70
60
*
*
Thickness of back
fat
* Significant differences
*
*
Thickness of
muscle meat
Control = 100%
Sangrovit (30 ppm)
Proportion of
lean meat
Effect of Sangrovit on Lean Meat Percentage
(Average of trials from 2002 – 2008)
9 of 12 trials show an improved lean meat
percentage due to Sangrovit supplementation
Average: + 0.7% more lean meat

Anti-inflammatory effects of Sangrovit
► Literature data and own studies (next slide) show anti-
inflammatory properties of Sangrovit
Where are these effects relevant?
Sangrovit is not absorbed from the intestine → local effects
the gut mucosa
Impact of anti-inflammatory properties
healthier intestinal mucosa ► better absorption of nutrients,
minerals… ► lower demand of nutrients to fight against
infections/inflammation
promoting immune system ► intestinal tract is the biggest immune
organ in the body
Effect of Sangrovit and indomethacine
on inflammation processes
Inflammatory processes
control =100%
0
-10
-20
-30
-40
-50
(Lenfeld et al. 1981)
Sangrovit QBA
Indometacine
0 1 2 3
Time (h)

Anti-inflammatory effects of Sangrovit
Effects of Sangrovit on mucosa integrity
(Prof. W. Gebreyes, North Carolina State University, USA, 2006)
Method: Evaluation (In vitro; Ussing chambers) of Transepithelial Electrical
Resistance (TER) as a parameter of mucosa thickness, integrity and
inflammation processes.
► Higher TER in the Sangrovit treatment indicates less
inflammation and lesions and a healthier gut mucosa in general.
Effects of Sangrovit on inflammation parameters
(M. Quereshi, North Carolina State University, USA, 2004)
Method: Determination of microphage nitrite production as an indicator of
inflammatory processes.
► Dose-dependent decrease in nitrite production
► Less inflammation with higher concentration of Sangrovit
TER (ohms/cm²)
80
70
60
50
40
30
20
10
0
Sangrovit
(50 mg/kg)
CTC
(60 mg/kg)

Effect of Sangrovit on Gut Morphology
Sangrovit cause a -20% lower weight of the intestine.
► Thinner intestinal wall
► Reduced inflammation
► Better Absorption of nutrients
At the same time the length was 10% higher.
► More absorption surface
► Higher capacity for synthesis of immune cells
Chang Won Kang, Ph.D. Konkuk University Seoul, Korea, (2008)

Anti-inflammatory effects of Sangrovit
Effect of Sangrovit on absorption capacity
Dr. J. Hoffmann, Agroproduct GmbH, Germany (2005)
Method: Evaluation of yolk colour as parameter of absorption capacity
Method of analyses
* Significant difference (p

Feed/Feeding
Health Performance

Impact of inflammation on protein turnover in different organs
Mercier, s. et al. (2002). J. Nutr. 132, 1921-1928
180
%
140
120
100
80
60
40
20
0
Control = 100%
Inflammation
(=Colitis)
IIeum Colon Muscle
Liver
Chronic intestinal inflammations (e.g. colitis) increase the protein turn over/requirement of the
intestinal mucosa at the expense muscle protein formation and daily growth .

Nutrients/Protein/AS requirement
Deficit
(Nutrients,
AS…)
Nutrients/Protein/AS delivered by the diet
► decreased performance
► reduces health status
► higher mortality
Stress /Infection pressure/Health problems…
Sangrovit
in the diet
2
2
Reduced
deficit
► less reduction in performance
► healthier animals
► reduced mortality
low high low
low high
low
1
Stress /Infection pressure/Health problems…
Lower and shorter infection processes due to Sangrovit‘s
anti-inflammatory effects
1
Higher protein/amino acid availability from the feed due to
Sangrovit (amino acid protection; increased nutrient availability,
better nutrient absorption)

Sangrovit during a three week lactation
Objective
Does Sangrovit increase the litter weight at weaning?
Method:
Place: farm with 400 sows in Germany, 2003
Treatments: Control; Sangrovit 30 mg/kg
Animals: 105 sows (parity 1 – 9)
Diet: 13.8 MJ/kg; 17.5 % CP; 9.0 g Lys/kg
Parity (lactation number) class
PC1 = Parity 1
PC2 = Parity 2
PC3 = Parity 3-6
PC4 = Parity 7-9

Sangrovit during a three week lactation
Litter weight gain [kg] – All sows (PC 1-4) LSmeans ± SEM
Day 1-10*
Day 11-20*
Day 1-20*
C (n=35)
21.4 ± 0.7
22.8 ± 0.7
44.0 ± 1.1
* T x PC (Treatment x Parity class) effect p

Sangrovit during a three week lactation
60
kg
50
45
40
35
30
Litter weight gain [kg] in first and second litter sows
First Litter Sows (PC 1)
C S30
(PC1 and 2; LSmeans ± SEM)
+ 10%
60
kg
50
45
40
35
30
Second Litter Sows (PC 2)
a
a,b significant differences
+13 %
+ 13,5%
b
C S30
Higher litter weight gain was observed in first and second litter sows treated with Sangrovit.
Sangrovit has a positive effect particularly in young sows that are typically affected by two
types of stress at the same time: Their own growth and milk production.
a
b

Sangrovit in combination with Organic acids (1)
Britsh Quality Pork, Great Britain 2007
Trial duration: 64 days (45-110 kg body weight)
Treatment:
control
control + Benzoic acid (5 kg/t) (+BA)
control + Benzoic acid (5 kg/t) + Sangrovit (30 mg/kg) (+BA + Sangrovit)
4 recurrences per treatment
(4 x 5 animals, 2 sows and 3 boars respectively)

Sangrovit in combination with Organic acids (2)
Effect of Sangrovit on fattening performance
(Total fattening period 48-110 kg, 64 d)
Total weight gain, 64 days of fattening
control: 60,15 kg, control + BA:61,3 kg, control + BA + Sangrovit: 66,62 kg

Summary: Effect of Sangrovit in Growing/Fattening pigs 1
Daily weight gain
(in relation to the control group = 100%)
Sangrovit
+3.3%
1 Average of 30 trials around the world over last 6 years
FCR
(in relation to the control group = 100%)
Sangrovit
- 3.8%

Sangrovit for Piglets – Meta-Analysis (1) 1
Effect on Fed intake
1 Results of 5 trials on efficacy of Sangrovit ® in weaned piglets: IRTA , Spain, 2007/2008; IRTA, Spain, 2008; Provimi, Netherlands, 2007;
UWM, Poland, 2008; Kaposvar, Hungary, 2008; Treatment groups: Control, Sangrovit 15 mg/kg; Sangrovit 30 mk/kg; Sangrovit 60 mg/kg
2 Sangrovit = S
2

Sangrovit for Piglets – Meta-Analysis (2) 1
Effect on Weight gain
1 Results of 5 trials on efficacy of Sangrovit ® in weaned piglets: IRTA , Spain, 2007/2008; IRTA, Spain, 2008; Provimi, Netherlands, 2007;
UWM, Poland, 2008; Kaposvar, Hungary, 2008; Treatment groups: Control, Sangrovit 15 mg/kg; Sangrovit 30 mk/kg; Sangrovit 60 mg/kg
2 Sangrovit = S
2

Sangrovit for Piglets – Meta-Analysis (3) 1
Effect on FCR
1 Results of 5 trials on efficacy of Sangrovit ® in weaned piglets: IRTA , Spain, 2007/2008; IRTA, Spain, 2008; Provimi, Netherlands, 2007;
UWM, Poland, 2008; Kaposvar, Hungary, 2008; Treatment groups: Control, Sangrovit 15 mg/kg; Sangrovit 30 mk/kg; Sangrovit 60 mg/kg
2 Sangrovit = S
2

Summary: Sangrovit for Piglets
Daily weight gain
(in relation to the control group = 100%)
Sangrovit
+ 4.4%
FCR
(in relation to the control group = 100%)
Sangrovit
- 3.3%

Sangrovit is a clear defined standardized product
Well described mode of action
Health promoting properties lead to an increased performance
Anti-inflammatory effects
Liver protection
Amino acid sparing effect
Improved FCR
Higher daily weight gain
Higher carcass value and quality
Constant performance and stability

Thank you very much for
your attention!
Don’t worry…. Be happy with Sangrovit!