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Summary of applications Sample Handling 187 Application Product Page Application tips for PCR The table to the left shows a selection of particular applications from the PCR area and serves as an overview. The complete product portfolio of the Eppendorf ® PCR System is found on the following pages. A selection of particular PCR products shown on the following pages, can be provided as Customized Solutions. Further information on page 380. Register your Eppendorf products online now to benefit from numerous advantages! You receive bonus ep-points immediately for each Eppendorf device registered. You can also easily manage your devices online and are always kept up-to-date on any news. epGreen ® Initiative ‡ Noise emission: Eppendorf PCR instruments are optimized to minimize noise emission ‡ PCR consumables are made of virgin Polypropylene and Polycarbonate that is fully recyclable ‡ All cardboard packaging is 100 % recyclable ‡ Wherever possible, water-soluble laquer or paints without heavy metals are used ‡ Mini-Satellite-system: By controlling up to 5 units of the Mastercycler ® pro with only 1 Control Panel, you can increase your PCR capacity by only increasing the parts needed, i.e. the thermo module. There is no need to buy an entire additional system to increase your capacity Mastercycler nexus, Mastercycler nexus gradient, Mastercycler nexus eco Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser’s own use under certain non real-time thermal cycler patents licensed from Applied Biosystems, LLC. Mastercycler ep realplex This is a Licensed Real-Time Thermal Cycler or Licensed Real-Time Temperature Cycling Instrument under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed expressly, by implication or by estoppel under any other patent claim. customized products p. 380 ‡ Connect up to 2 Mastercycler nexus eco units to your existing unit to acheive low energy consumption ‡ Stand-by function of the Mastercycler nexus saves energy For more information on epGreen, see pages 398 and 399. In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: info@eppendorf.com · www.eppendorf.com Summary of Applications 4
Thermocyclers 4 188 Sample Handling Mastercycler® pro ® vapo.protect ® technology* Evaporation in a PCR reaction vessel not only limits the ability to work with low reaction volumes but also makes it difficult to obtain reproducible results. For the Mastercycler pro it has been Eppendorf’s aim to protect the PCR reaction from evaporation. While common PCR instruments press a metal plate onto the PCR consumable, the vapo.protect lid covers the PCR consumable with a cushion that conforms to the shape of the consumable—no matter which consumable you use. The snug fit of the cushion in combination with the high pressure and excellent heating capabilities of the lid reduce evaporation to a minimum. Evaporation leads to increasing concentrations of the PCR master mix components, especially primers. This, in turn, leads to non-specific binding. The changes in concentrations due to evaporation both during the experiment and between experiments can be a cause for great concern for any scientist who tries to keep variables to a minimum. Gradient with SteadySlope ® technology The gradient option of the Mastercycler pro features SteadySlope technology to ensure that the heating and cooling ramp rates are identical in both gradient and normal operation. Thus, you can expect identical temperature control characteristics during both optimization and routine experiments. Programming the gradient with the Control Panel or the CycleManager pro is easy and intuitive—so that novice users can get started quickly and safely. *Patents pending Order from your laboratory supply dealer. Product appearance, specifications, and/or prices are subject to change without notice. vapo.protect technology leads to improved reproducibility and specificity at the rim and in the corners of the block Certified Quality – Individual, documented quality control certificate – Calibration according to national and international standards: NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK) 4 | Instruments | PCR Description Fastest speeds, highest precision, easy to use and absolute reliability combined in a flexible concept—that is the Mastercycler pro. The requirements for PCR are defined by research projects that are continually evolving. As the needs of the researchers change the instrument they use needs to be adaptable. That is why only an open and flexible system fits into today’s lab. Eppendorf’s Mastercycler pro offers ultimate flexibility: – choose from three block formats: 96, 96 Silver and 384 – operate the Mastercycler pro in various configurations – use any plate, tube or strip that you choose Unsurpassed speed can be achieved with Eppendorf’s Impulse PCR, a device driven hot-start function that increases heating rates to 8 °C/s. For additional information, see application tips for PCR on page 393.
Page 1 and 2: 4 A History of Advanced PCR 184 Sam
Page 3: 4 Summary of Applications 186 Sampl
Page 7 and 8: Thermocyclers 4 190 Sample Handling
Page 17 and 18: 4 PCR Plates 200 Sample Handling tw
Page 21 and 22: 4 PCR Tubes 204 Sample Handling PCR
Page 25 and 26: 4 Heat Sealing Materials 208 Sample
Page 27 and 28: 4 Heat Sealing Materials 210 Sample
Page 29 and 30: 4 Adapters 212 Sample Handling in s
Page 31 and 32: Services - PCR 44 214 Sample Servic
Page 33: Support and Services Directory Cont

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