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Download - Eppendorf Canada

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Thermocyclers<br />

4<br />

198 Sample Handling<br />

Mastercycler® ep realplex<br />

‡ Endpoint<br />

The analysis module option for endpoint<br />

measurements instructs Mastercycler ep<br />

realplex to serve as a “plate fluorometer”<br />

and aids in the determination of absolute<br />

fluorescence intensities, even with samples<br />

previously amplified on standard thermocyclers.<br />

‡ +/– Assay<br />

Define critical threshold values through this<br />

analysis module option to differentiate between<br />

positive and negative samples (e.g., for the<br />

detection of pathogens). Data from previous<br />

endpoint measurements serves as the source<br />

for such determinations.<br />

The Mastercycler ep realplex software includes various analysis modules making this system versatile and ideal<br />

for multiple applications. Protein characterization can now be added to this list; with the use of our “Inverted Data”<br />

tool all protein reactions can be easily evaluated with our system (see images below).<br />

‡ Protein Characterization<br />

Inverted data flips the negative peaks so the threshold is no longer below zero and the data may be properly evaluated.<br />

Licensed for real-time PCR, see page 196.<br />

Order direct from <strong>Eppendorf</strong> <strong>Canada</strong> Ltd.<br />

Product appearance, specifications, and/or prices are subject to change without notice.

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