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Mastercycler® ep realplex
Eppendorf’s Mastercycler ep realplex software is intuitive and
user-friendly. The Windows ® -like interface allows for a clear
and comprehensive organization of multiple viewing windows.
The Plate layout screen is designed like a 96-well PCR plate
making labeling and identification easy. This screen also
provides a helpful “Hint” box in the bottom corner making
set up simple for users of any level. Once your experiment
has begun the Monitoring screen allows you to visualize the
results in real-time as they appear, and you can interrupt the
run once the desired results have been obtained!
‡ Plate layout
The clear and comprehensive organization of
multiple viewing windows, as well as intuitive
options for selection within each view, enable
easy and fast plate setup when creating assays.
‡ Quantification/relative quantification
This analysis module performs calculations
from raw data for a variety of assays, including
absolute quantification of DNA or relative
quantification of gene expression based
on the ADCt method.
Sample Handling 197
The Mastercycler ep realplex software is designed to be
intuitive for real-time PCR users of all levels, making data
collection and analysis easy, efficient and comprehensive.
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 | Instruments | PCR
‡ Raw data
This software module displays the generated
raw data in real-time, which means that you
can immediately evaluate your real-time PCR
and interrupt the reaction as soon as the
desired result is obtained.
‡ Gene identification
Selecting this analysis module option generates
allele-discrimination results. Data previously
generated in melting curve analyses, endpoint
measurements or Ct-value determinations
serves as the basis.
4 Thermocyclers