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Mastercycler® ep realplex Product features High-speed real-time PCR ‡ Load your plate and receive analyzed results in less than half an hour! ‡ Fast and easy programming ‡ Fast optical detection capabilities eliminate the need for lengthy extension/elongation ‡ Aluminum block model offers faster ramp rates than nearly any other supplier ‡ “S” model for highest speed ‡ Complete more experiments per day No restrictions ‡ Use the reagents, kits and consumables of your choice ‡ Even the high-speed “S” model does not lock you in to special kits or plates Gradient ‡ Optimize your protocols in a single plate/experiment ‡ Test more distinct temperatures at once with Triple Circuit thermal block design Application * New! Modified slip cover (light-transmissive cover for the optical openings of the heated plates assembly, US patent pending). Licensed and authorized for real-time PCR; see next page. ‡ Quantitative real-time PCR 96-well silver block Sample Handling 195 96-well aluminum block Description Continuing the Eppendorf ® focus on best design, the Mastercycler ep realplex combines the highest performance with the most advanced features and capabilities of a 96-well real-time PCR system—and in a compact class! Highly sensitive optics ‡ The new optical system of the Mastercycler ep realplex offers a new, exclusive Particle Protection* and Photomultipliers of the latest generation Highest reproducibility ‡ LEDs keep consistent intensity over time—other light sources lose intensity over time ‡ Each LED is normalized to equal intensity ‡ Over 55 years of Eppendorf expertise in optical systems Intuitive software ‡ All analysis modules included (see pages 197 and 198) ‡ Highly accurate calculation of Ct-values using the CalQplex ® algorithm ‡ “QUICK START” capability Eppendorf design ‡ Quiet operation, 554 dB(A) ‡ Small footprint In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 E-mail: info@eppendorf.com · www.eppendorf.com 4 Thermocyclers
Thermocyclers 4 196 Sample Handling Mastercycler® ep realplex Complete real-time PCR system twin.tec ® real-time PCR plate 96 individual LED array realplex filter wheels Mastercycler ep realplex 2 and realplex 2 S ‡ These two-channel systems meet the needs of most laboratories (emission 520/550 nm) ‡ Designed for use with SYBR ® Green assays, FAM-labeled probes or single and duplex probe reactions ‡ High reproducibility is possible at 10 μL total reaction volumes with cali brated Eppendorf ® pipettes; for lower volumes please inquire about automated real-time PCR set-up ‡ 2-year warranty comes standard—best in its class! ‡ Lifetime warranty on LED array Mastercycler ep realplex 4 and realplex 4 S ‡ This four-channel system (emission 520/550/580/605 nm) provides full flexibility for multiplexing ‡ High reproducibility is possible at 10 μL total reaction volumes with cali brated Eppendorf pipettes; for lower volumes please inquire about automated real-time PCR set-up ‡ 2-year warranty comes standard—best in its class! ‡ Lifetime warranty on LED Practice of the patented polymerase chain reaction (PCR) process requires a license. The Eppendorf [or appropriate trademark] Thermal Cycler is an Authorized Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. This is a Licensed Real-Time Thermal Cycler under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed expressly, by implication or by estoppel under any other patent claim. The purchase of this product includes a limited, non-transferable Iicense under U.S. Patent No. 5,871,908 and all continuations and divisionals, and corresponding claims in patents and patent applications outside the United States, owned by Roche Diagnostics GmbH, for internal research use or for non-in vitro diagnostics applications with authorized reagents with regard to Melting Curve Analysis. No right is conveyed, expressly, by implication or estoppel, under any other patent or patent claims owned by Roche Diagnostics GmbH, or by any other Party. Order direct from Eppendorf Canada Ltd. Product appearance, specifications, and/or prices are subject to change without notice.
Page 1 and 2: 4 A History of Advanced PCR 184 Sam
Page 3 and 4: 4 Summary of Applications 186 Sampl
Page 5 and 6: Thermocyclers 4 188 Sample Handling
Page 11: Thermocyclers 4 194 Sample Handling
Page 17 and 18: 4 PCR Plates 200 Sample Handling tw
Page 21 and 22: 4 PCR Tubes 204 Sample Handling PCR
Page 25 and 26: 4 Heat Sealing Materials 208 Sample
Page 27 and 28: 4 Heat Sealing Materials 210 Sample
Page 29 and 30: 4 Adapters 212 Sample Handling in s
Page 31 and 32: Services - PCR 44 214 Sample Servic
Page 33: Support and Services Directory Cont

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