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4 A History of Advanced PCR 

184 Sample Handling 

Quality is timeless 

Eppendorf ® is an innovator, designing instruments that 

fit the needs of today’s scientist. The Mastercycler ® 

gradient by Eppendorf—still popular today—was 

the first available gradient thermal cycler. Today the 

gradient function remains critical for the optimization 

of a PCR reaction. 

The next generation Mastercycler ep is a lab favorite 

and still one of the fastest cyclers around. Given this 

history of innovation, it’s only natural that Eppendorf 

would now introduce the latest in evaporation reduction 

technology—vapo.protect ® . With a shift to low volume 

reactions and faster protocols, the need to safeguard 

the integrity of your sample has become increasingly 

vital. The Mastercycler pro does just that. 

Survival of the Fittest— 

In the age of networking, the new Mastercycler nexus is 

your reliable companion when it comes to daily routine 

PCR. With its ease-of-use, small footprint, energy saving 

features and its’ capability to notify you by e-mail when it 

is finished—what else do you need from a cycler? 

Eppendorf has also addressed the needs of real-time 

PCR. Integrating the advantages of the Mastercycler ep 

gradient family, we have developed a fully licensed 

real-time PCR system that meets the requirements 

of the latest quantitative PCR applications. 

A history of advanced PCR 

‡ Microcycler: Eppendorf 

introduces its first thermal 

cycler using water to heat 

and cool. 

‡ Mastercycler 5330: 

Eppendorf introduces the 

first Mastercycler based 

on peltier technology. 

1990 1993 

Faster speeds, highest precision, excellent temperature 

control stability, extremely user-friendly, and absolute 

reliability united with a flexible concept: these are the 

characteristics of the Mastercycler families. 

‡ Mastercycler gradient: 

the first gradient thermal 

cycler on the market. 

‡ Thin-walled 0.2 mL 

PCR tubes allow for 

rapid heat transfer. 

‡ Dual component twin.tec ® 

plates provide a stable 

frame and extremely 

thin-walled wells for 

optimum heat transfer. 

1997 1998 2002

‡ Mastercycler ep: 

Fast heating/cooling rates 

and ultra quiet operation— 

in a compact design. 

2003 2005 

The Mastercycler® Family 

‡ Mastercycler ep 

realplex: Extremely 

fast optics for rapid 

data acquisition. 

Sample Handling 185 

Then... Now—always providing quality solutions for your lab. 

‡ Mastercycler pro: 

New vapo.protect ® 

technology reduces 

evaporation. ‡ qPCR consumables 

and divisible twin.tec 

PCR plates. 

‡ Mastercycler nexus: 

Connects to network 

to send status updates 

through e-mail. 

2008 2010 Today 

4 A History of Advanced PCR

4 Summary of Applications 


Summary of applications 

Application 

Instruments 

Product Page 

Conventional PCR Mastercycler ® pro 

188 

Mastercycler nexus Family 

192 

Real-time PCR Mastercycler ep realplex 195 

PCR optimization Mastercycler pro 

188 

Mastercycler nexus gradient 

194 

in situ PCR Mastercycler nexus 

192 

Mastercycler nexus gradient with in situ Adapter 212 

Mastercycler nexus flat 

194 

Cycle Sequencing Mastercycler pro 

188 

Mastercycler nexus Family 

192 

Heat sealing of 96- and 384-well PCR plates 

Consumables 

Tubes, Plates and Accessories 

Heat Sealer 208 

PCR with sample throughput 0.2/0.5 mL PCR Tube, thin-walled 204 

PCR with medium sample throughput 0.2 mL PCR Tubes in strips of 8 

0.1 mL PCR Tubes in strips of 8 

twin.tec ® 205 

205 

PCR Plate 96, unskirted, divisible 201 

PCR with medium to high sample throughput twin.tec PCR Plate 96, semi-skirted 

200 

twin.tec PCR Plate 96, skirted 

200 

PCR with medium to high sample throughput, 

usage in automatic devices 

twin.tec PCR Plate 96, skirted 200 

PCR with high sample throughput, 

twin.tec PCR Plate 384 

200 

usage in automatic devices 

twin.tec real time PCR Plate 384 

206 

Real-time PCR twin.tec real-time PCR Plates 

206 

real-time PCR Tube Strips 

207 

PCR with bacterial templates 

Accessories 

twin.tec microbiology PCR Plate 201 

Non-cooled PCR Rack, work tray and frame 211 

Cooled (0 °C and –21 °C) IsoTherm-System ® 124 

Cooled (0 °C), color change for temperature control 

Sealing options 

PCR-Cooler 125 

Partial seal for real-time PCR and low volume PCR Masterclear ® Cap Strips 207 

Partial seal of PCR plates and PCR strips Cap Strips, Capping Aid 210 

Heat sealing, transparent, non-removable, 

cannot be pierced (e.g. for colorimetric evaluation) 

Heat Sealing Film 208 

Heat sealing, non-transparent, removable, 

can be pierced 

Heat Sealing Foil 208 

Adhesive seal, transparent, removable, 

cannot be pierced 

PCR Film 209 

Adhesive seal, transparent, removable, 

cannot be pierced 

PCR Foil 209 

Adhesive seal, transparent, optically clear, removable, cannot be pierced Masterclear real-time PCR Film 210 

4 | Info | PCR 

Practice of the patented polymerase chain reaction (PCR) process requires a license. The Mastercycler is an Authorized Thermal Cycler and 

may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in 

accordance with the label rights accompanying such reagents. 

This product is sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc. and Applied Biosystems. 

Product appearance, specifications, and/or prices are subject to change without notice.

Summary of applications 


Application Product Page 

Application tips for PCR 

The table to the left shows a selection of particular applications from the 

PCR area and serves as an overview. The complete product portfolio of 

the Eppendorf ® PCR System is found on the following pages. 

A selection of particular PCR products shown on the following pages, can 

be provided as Customized Solutions. Further information on page 380. 

Register your Eppendorf products online now to benefit from numerous 

advantages! You receive bonus ep-points immediately for each Eppendorf 

device registered. You can also easily manage your devices online and are 

always kept up-to-date on any news. 

epGreen ® Initiative 

‡ Noise emission: Eppendorf PCR instruments are optimized 

to minimize noise emission 

‡ PCR consumables are made of virgin Polypropylene and 

Polycarbonate that is fully recyclable 

‡ All cardboard packaging is 100 % recyclable 

‡ Wherever possible, water-soluble laquer or paints without 

heavy metals are used 

‡ Mini-Satellite-system: By controlling up to 5 units of the 

Mastercycler ® pro with only 1 Control Panel, you can 

increase your PCR capacity by only increasing the parts 

needed, i.e. the thermo module. There is no need to buy 

an entire additional system to increase your capacity 

Mastercycler nexus, Mastercycler nexus gradient, 

Mastercycler nexus eco 

Purchase of this instrument conveys a limited non-transferable 

immunity from suit for the purchaser’s own use under certain 

non real-time thermal cycler patents licensed from Applied 

Biosystems, LLC. 

Mastercycler ep realplex 

This is a Licensed Real-Time Thermal Cycler or Licensed 

Real-Time Temperature Cycling Instrument under Applera’s 

United States Patent No. 6,814,934 and corresponding claims 

in non-U.S. counterparts thereof, for use in research and for all 

other applied fields except human in vitro diagnostics. No right 

is conveyed expressly, by implication or by estoppel under any 

other patent claim. 

customized 

products 

p. 380 

‡ Connect up to 2 Mastercycler nexus eco units to your 

existing unit to acheive low energy consumption 

‡ Stand-by function of the Mastercycler nexus saves energy 

For more information on epGreen, see pages 398 and 399. 

In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715 

E-mail: info@eppendorf.com · www.eppendorf.com 

Summary of Applications 

4

Thermocyclers 

4 


Mastercycler® pro 

® 

vapo.protect ® technology* 

Evaporation in a PCR reaction vessel not only limits the ability 

to work with low reaction volumes but also makes it difficult to 

obtain reproducible results. For the Mastercycler pro it has been 

Eppendorf’s aim to protect the PCR reaction from evaporation. 

While common PCR instruments press a metal plate onto 

the PCR consumable, the vapo.protect lid covers the PCR 

consumable with a cushion that conforms to the shape of the 

consumable—no matter which consumable you use. The snug 

fit of the cushion in combination with the high pressure and 

excellent heating capabilities of the lid reduce evaporation to 

a minimum. 

Evaporation leads to increasing concentrations of the PCR 

master mix components, especially primers. This, in turn, 

leads to non-specific binding. The changes in concentrations 

due to evaporation both during the experiment and between 

experiments can be a cause for great concern for any scientist 

who tries to keep variables to a minimum. 

Gradient with SteadySlope ® technology 

The gradient option of the Mastercycler pro features 

SteadySlope technology to ensure that the heating and 

cooling ramp rates are identical in both gradient and normal 

operation. Thus, you can expect identical temperature control 

characteristics during both optimization and routine experiments. 

Programming the gradient with the Control Panel or the 

CycleManager pro is easy and intuitive—so that novice users 

can get started quickly and safely. 

*Patents pending 

Order from your laboratory supply dealer. 

Product appearance, specifications, and/or prices are subject to change without notice. 

vapo.protect 

technology leads to improved 

reproducibility and specificity 

at the rim and in the corners 

of the block 

Certified Quality 

– Individual, documented quality control certificate 

– Calibration according to national and international standards: 

NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK) 

4 | Instruments | PCR 

Description 

Fastest speeds, highest precision, easy to use and absolute 

reliability combined in a flexible concept—that is the 

Mastercycler pro. 

The requirements for PCR are defined by research projects that 

are continually evolving. As the needs of the researchers change 

the instrument they use needs to be adaptable. That is why only 

an open and flexible system fits into today’s lab. Eppendorf’s 

Mastercycler pro offers ultimate flexibility: 

– choose from three block formats: 96, 96 Silver and 384 

– operate the Mastercycler pro in various configurations 

– use any plate, tube or strip that you choose 

Unsurpassed speed can be achieved with Eppendorf’s Impulse 

PCR, a device driven hot-start function that increases heating 

rates to 8 °C/s. 

For additional information, see application tips for PCR 

on page 393.


Technical specifications 


Description Mastercycler pro Mastercycler pro S Mastercycler pro 384 

Thermoblock Aluminum Silver Aluminum 

Sample capacity 96 x 0.2 mL PCR tubes or 1 PCR plate 8 x 12 (unskirted, 

semi-skirted, skirted—according to SBS standard) 

Temperature control range of the block 4–99 °C 4–99 °C 4–99 °C 

1 PCR plate 384 

Temperature control mode Fast, Standard, Safe ; all available in gradient mode 

Heating technology of the block Peltier Elements, Triple Circuit Technology 

Gradient block Over 12 columns Over 12 columns Over 24 columns 

Gradient range 1–20 °C 1–24 °C 1–20 °C 

Gradient temperature range 30–99 °C 30–99 °C 30–99 °C 

Lid temperature range 37–110 °C 37–110 °C 37–110 °C 

Lid descent and closing pressure vapo.protect ® technology, with Thermal Sample Protection 

Block homogeneity 20–72 °C ±0.3 °C, 

90 °C ±0.4 °C 

Control accuracy ±0.2 °C ±0.2 °C ±0.2 °C 

Heating rate* approx. 4 °C/s approx. 6 °C/s approx. 4 °C/s 

Cooling rate* approx. 3 °C/s approx. 4.5 °C/s approx. 3 °C/s 

Interfaces 1 x Centronics ® , 1 x RS-232, Control panel, one each of CAN_in/CAN_out 

Dimensions (W x D x H) 10.2 x 16.3 x 14.6 in/ 

26 x 41.5 x 37 cm 

Product features 

‡ Extremely fast heating and cooling rates— 

get results faster and do more in one day 

‡ Ultimate reduction of evaporation for reliable results 

‡ Gradient blocks with SteadySlope ® technology for 

protocol optimization 

‡ Intuitive graphic user interface 

‡ Display to indicate cycler number in a network 

‡ Upgradable to real-time PCR 

‡ 2-year warranty 

‡ Optional self-test dongle available to test functionality 

of the Peltier Elements 

‡ Stand alone system fits on virtually every lab bench 

and is operated by a control panel 

‡ Expandable to a Mini Satellite System or network 

– Mini Satellite System: operated by Control Panel. 

Control up to 5 units with one Control Panel. 

Combine any block format. Expandable to a network. 

– Computer Network: combines up to 30 units for 

ultimate throughput. Operated by a computer and the 

CycleManager pro software. Benefit from a client/ 

server architecture and full software functionality. 

10.2 x 16.3 x 14.6 in/ 

26 x 41.5 x 37 cm 

10.2 x 16.3 x 14.6 in/ 

26 x 41.5 x 37 cm 

Weight 40.8 lbs /18.5 kg 40.8 lbs /18.5 kg 40.8 lbs /18.5 kg 

Power supply 120 V, 50–60 Hz 120 V, 50–60 Hz 120 V, 50–60 Hz 

Power consumption 950 W 950 W 950 W 

* Block temperature 

This product is licensed under U.S. patent Nos. 5,525,300, 5,779,981 and 6,054,263. The heated cover device is licensed under US 5,552,580 and foreign equivalents. The user of the Eppendorf Mastercycler pro 

might require additional rights for kits, reagents and other components required for his/her application. Such accompanying rights for these kits, reagents and other may be obtained by the respective holder 

of such rights. No rights are conveyed expressly, by implication or estoppel to any patents on real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent or kit. 

Mastercycler pro upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts. 



4 Thermocyclers

Thermocyclers 

4 



Control panel programming features 

‡ Highest degree of flexibility, graphical user interface 

‡ Variable ramp rates 

‡ Simulation mode for temperature control speed and 

protocols of the original Mastercycler family— 

heating 3 °C/s, cooling 2 °C/s 

‡ Time and temperature increments 

‡ Pause command 

‡ Programmable alarm sound 

‡ Freely configurable auto-restart dialogue 

Regulatory documentation 

Compliance with GMP/GLP guidelines is becoming increasingly 

important, particularly in the pharmaceutical and biotechnology 

industries. The Mastercycler pro system’s programming and user 

management system provides a GLP-conforming environment 

for your PCR assays. 

Technical specifications of the Control Panel 

Number of Mastercycler pro cyclers that can be controlled 1–5 

Memory capacity 16 MB, >700 programs 

Memory expansion MultiMediaCard reader and 2 x USB port 2.0 

Number of user-defined folders >100 

Dimensions (W x D x H) 10.0 x 6.6 x 4.4 in/25 x 16.5 x 11 cm 

Interfaces 2 x USB port 

Weight 2.6 lb/1.2 kg 

Power supply, consumption Via the connected thermal module 



Program and user administration features 

‡ One administrator to manage user and service functions 

‡ Easy addition of new users with limited rights 

‡ Ability for Mastercycler pro users to change their 

passwords; no administrator access to user passwords 

‡ Password protection against unauthorized login 

‡ A report file for each run, including total temperature 

profiling 

‡ Development, testing and validation of the software 

using certified standards 


might require additional rights for kits, reagents and other components required for his/her application. Such accompanying rights for these kits, reagents and other may be obtained by the respective holder of such 

rights. No rights are conveyed expressly, by implication or estoppel to any patents on real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent or kit. Mastercycler pro 

upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts.

CycleManager pro for Mastercycler® pro 

Ordering information 



‡ Network and control up to 30 Mastercycler pro 

thermal cyclers with a PC; mix and match models 

of choice 

‡ Multi-Mastercycler-Networking with a PC 

‡ Intuitive Windows ® -like interface 

‡ Icon-based creation and edit of programs 

‡ Simultaneous control of all or selected 

Mastercycler pro cyclers 

‡ GLP-conformity documentation of all PCR experiments 

‡ User password protection 

‡ Real-time acquisition of temperature data 

‡ Easy installation and uninstallation 

‡ Compatible with Windows 

‡ Easy transfer of PCR programs from the Control Panel 

to the PC 

Description 

Complete Mastercycler pro package (includes cycler and control panel) 

Catalog No. 

Mastercycler pro and Control Panel, 120 V, 50–60 Hz 950040015 

Mastercycler pro S and Control Panel, 120 V, 50–60 Hz 950040025 

Mastercycler pro 384 and Control Panel, 120 V, 50–60 Hz 

Individual Mastercycler pro modules 

950040035 

Control Panel, includes connection cable 950030050 

Mastercycler pro*, 120 V, 50–60 Hz 950030010 

Mastercycler pro S*, 120 V, 50–60 Hz 950030020 

Mastercycler pro 384*, 120 V, 50–60 Hz 

Accessories 

950030030 

CAN_Bus connection cable, 50 cm 950014008 

CAN_Bus connection cable, 150 cm 950014016 

Self Test Dongle 950030040 

MultiMediaCard, 64 MB 960002008 

CycleManager pro, incl. installation manual, online help, and connection cable 

Service 

Extended Warranty, see page 215 for details 

Preventive Maintenance, see page 215 for details 

950017007 

* A Control Panel or CycleManager pro software (both sold separately) is required for operation; CAN_Bus connection cables are required to link cyclers together as a network. 


might require additional rights for kits, reagents and other components required for his/her application. Such accompanying rights for these kits, reagents and other may be obtained by the respective holder of such 

rights. No rights are conveyed expressly, by implication or estoppel to any patents on real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent or kit. Mastercycler pro 

upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts. 



4 Thermocyclers

Thermocyclers 

4 


Mastercycler® nexus 

NEW! 

‡ Stand-by function saves energy 

‡ Graphical user interface 

‡ USB port for data transfer 

or mouse connection 



‡ E-mail notification


‡ One hand operation 

‡ flexlid adjusts to 

height of consumables 

‡ Connect up to 2 Mastercycler nexus eco 

units to increase throughput 




4 Thermocyclers

Thermocyclers 

4 



Description 

The Mastercycler nexus offers the reliability and ease-of-use 

of an Eppendorf cycler with low energy consumption, easy 

expandability to 3 units and a small footprint make it not only fit 

to your budget, but also to your precious lab space. It can handle 

all kinds of PCR consumables, from low volume all the way up 

to 0.5 mL PCR tubes. All common PCR plates fit—and if your 

throughput becomes even higher, you can just connect 1 or 

2 Mastercycler nexus eco to your Mastercycler nexus. 


‡ Flexlid concept allows use of different consumables 

with automatic height adjustment of the heated lid 

‡ Intuitive graphic programming 

‡ Small footprint 

‡ Universal block for ultimate flexibility 

‡ Connect up to 2 Mastercycler nexus eco units to your 

existing unit 

‡ Available with 12 column gradient 

‡ E-mail notification 

‡ Average PCR run power consumption of 0.154 kW/h 

Further information under 

www.eppendorf.ca/mastercyclernexus 

For additional information see “Application tips for PCR” 

on page 393. 


Description Catalog No. 

Mastercycler nexus gradient, 120 V, 50/60 Hz 6331000025 

Mastercycler nexus, 120 V, 50/60 Hz 6333000022 

Mastercycler nexus eco 1 , 120 V, 50/60 Hz 6332000029 

Mastercycler nexus flat block, 120 V, 50/60 Hz 6335000020 

Self test dongle 950030040 

CAN-Bus connection cable 1 , 50 cm 950014008 

CAN-Bus connection cable 1 , 150 cm 950014016 

1 Requires Mastercycler nexus gradient or Mastercycler nexus to operate. 



Gradient with SteadySlope ® Technology 

The gradient option of the Mastercycler nexus features the 

SteadySlope technology to ensure that the heating and 

cooling ramp rates are identical in both gradient and normal 

operation. Thus, you can expect identical temperature control 

characteristics in both optimization and routine experiments. 

Programming the gradient is easy and intuitive – so that novice 

users can get started quickly and safely. 

Easy testing 

The self test function of the Mastercycler nexus provides the 

ability to check the peltier elements of the thermal block easily. 

This feature tests the functionality of all six peltier elements and 

provides a corresponding certificate. This way, the user can 

document the homogeneity of the instrument between regular 

testing intervals. In addition, Eppendorf ® offers a Temperature 

Validation System as an external testing device. 

Certified Quality 

‡ Individual, documented quality control certificate 

‡ Calibration according to national and international standards: 

NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK) 

‡ UL/CUL listed 

High quality consumables 

Consumables of high quality, e.g. Eppendorf twin.tec ® PCR 

plates, help to improve PCR results. For example, the raised rims 

of the Eppendorf twin.tec PCR plates are optimized to guarantee 

tight sealing in all wells. For further information, please visit 

www.eppendorf.ca/pcr

Mastercycler® ep realplex 


High-speed real-time PCR 

‡ Load your plate and receive analyzed results in less than 

half an hour! 

‡ Fast and easy programming 

‡ Fast optical detection capabilities eliminate the need 

for lengthy extension/elongation 

‡ Aluminum block model offers faster ramp rates than 

nearly any other supplier 

‡ “S” model for highest speed 

‡ Complete more experiments per day 

No restrictions 

‡ Use the reagents, kits and consumables of your choice 

‡ Even the high-speed “S” model does not lock you in to 

special kits or plates 

Gradient 

‡ Optimize your protocols in a single plate/experiment 

‡ Test more distinct temperatures at once with Triple 

Circuit thermal block design 

Application 

* New! Modified slip cover (light-transmissive cover for the optical openings of the heated plates assembly, US patent pending). 

Licensed and authorized for real-time PCR; see next page. 

‡ Quantitative real-time PCR 

96-well 

silver block 


96-well 

aluminum block 

Description 

Continuing the Eppendorf ® focus on best design, the 

Mastercycler ep realplex combines the highest performance 

with the most advanced features and capabilities of a 96-well 

real-time PCR system—and in a compact class! 

Highly sensitive optics 

‡ The new optical system of the Mastercycler ep realplex 

offers a new, exclusive Particle Protection* and 

Photomultipliers of the latest generation 

Highest reproducibility 

‡ LEDs keep consistent intensity over time—other light 

sources lose intensity over time 

‡ Each LED is normalized to equal intensity 

‡ Over 55 years of Eppendorf expertise in optical systems 

Intuitive software 

‡ All analysis modules included (see pages 197 and 198) 

‡ Highly accurate calculation of Ct-values using 

the CalQplex ® algorithm 

‡ “QUICK START” capability 

Eppendorf design 

‡ Quiet operation, 554 dB(A) 

‡ Small footprint 



4 Thermocyclers

Thermocyclers 

4 



Complete real-time PCR system twin.tec ® real-time PCR plate 

96 individual LED array realplex filter wheels 

Mastercycler ep realplex 2 and realplex 2 S 

‡ These two-channel systems meet the needs of most 

laboratories (emission 520/550 nm) 

‡ Designed for use with SYBR ® Green assays, 

FAM-labeled probes or single and duplex 

probe reactions 

‡ High reproducibility is possible at 10 μL total 

reaction volumes with cali brated Eppendorf ® pipettes; 

for lower volumes please inquire about automated 

real-time PCR set-up 

‡ 2-year warranty comes standard—best in its class! 

‡ Lifetime warranty on LED array 

Mastercycler ep realplex 4 and realplex 4 S 

‡ This four-channel system (emission 520/550/580/605 nm) 

provides full flexibility for multiplexing 

‡ High reproducibility is possible at 10 μL total 

reaction volumes with cali brated Eppendorf pipettes; 

for lower volumes please inquire about automated 

real-time PCR set-up 

‡ 2-year warranty comes standard—best in its class! 

‡ Lifetime warranty on LED 

Practice of the patented polymerase chain reaction (PCR) process requires a license. The Eppendorf [or appropriate trademark] Thermal Cycler is an Authorized 

Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in 

accordance with the label rights accompanying such reagents. This is a Licensed Real-Time Thermal Cycler under Applera’s United States Patent No. 6,814,934 and 

corresponding claims in non-U.S. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed 

expressly, by implication or by estoppel under any other patent claim. 

The purchase of this product includes a limited, non-transferable Iicense under U.S. Patent No. 5,871,908 and all continuations and divisionals, and corresponding 

claims in patents and patent applications outside the United States, owned by Roche Diagnostics GmbH, for internal research use or for non-in vitro diagnostics 

applications with authorized reagents with regard to Melting Curve Analysis. No right is conveyed, expressly, by implication or estoppel, under any other patent or 

patent claims owned by Roche Diagnostics GmbH, or by any other Party. 

Order direct from Eppendorf Canada Ltd. 



Eppendorf’s Mastercycler ep realplex software is intuitive and 

user-friendly. The Windows ® -like interface allows for a clear 

and comprehensive organization of multiple viewing windows. 

The Plate layout screen is designed like a 96-well PCR plate 

making labeling and identification easy. This screen also 

provides a helpful “Hint” box in the bottom corner making 

set up simple for users of any level. Once your experiment 

has begun the Monitoring screen allows you to visualize the 

results in real-time as they appear, and you can interrupt the 

run once the desired results have been obtained! 

‡ Plate layout 

The clear and comprehensive organization of 

multiple viewing windows, as well as intuitive 

options for selection within each view, enable 

easy and fast plate setup when creating assays. 

‡ Quantification/relative quantification 

This analysis module performs calculations 

from raw data for a variety of assays, including 

absolute quantification of DNA or relative 

quantification of gene expression based 

on the ADCt method. 


The Mastercycler ep realplex software is designed to be 

intuitive for real-time PCR users of all levels, making data 

collection and analysis easy, efficient and comprehensive. 



4 | Instruments | PCR 

‡ Raw data 

This software module displays the generated 

raw data in real-time, which means that you 

can immediately evaluate your real-time PCR 

and interrupt the reaction as soon as the 

desired result is obtained. 

‡ Gene identification 

Selecting this analysis module option generates 

allele-discrimination results. Data previously 

generated in melting curve analyses, endpoint 

measurements or Ct-value determinations 

serves as the basis. 

4 Thermocyclers

Thermocyclers 

4 



‡ Endpoint 

The analysis module option for endpoint 

measurements instructs Mastercycler ep 

realplex to serve as a “plate fluorometer” 

and aids in the determination of absolute 

fluorescence intensities, even with samples 

previously amplified on standard thermocyclers. 

‡ +/– Assay 

Define critical threshold values through this 

analysis module option to differentiate between 

positive and negative samples (e.g., for the 

detection of pathogens). Data from previous 

endpoint measurements serves as the source 

for such determinations. 

The Mastercycler ep realplex software includes various analysis modules making this system versatile and ideal 

for multiple applications. Protein characterization can now be added to this list; with the use of our “Inverted Data” 

tool all protein reactions can be easily evaluated with our system (see images below). 

‡ Protein Characterization 

Inverted data flips the negative peaks so the threshold is no longer below zero and the data may be properly evaluated. 

Licensed for real-time PCR, see page 196. 





Optical module 

Excitation source 96 LEDs (470 nm) 

Emission filters 520/550 nm (realplex2 ) 

520/550/580/605 nm (realplex4 ) 

Detector 1 photomultiplier tube (realplex2 ) 

2 photomultiplier tubes (realplex4 ) 

Dynamic range 8 orders of magnitude from starting copy number 

Sensitivity 

Thermomodule 

≤50 fmol fluorescein 

Sample capacity 96 x 0.2 mL PCR tubes or one 96-well PCR plate 

(unskirted, semi-skirted, skirted—as per SBS format) 

Temperature control range of block 4–99 °C 

Degree range of gradient, maximum 1–20 °C (aluminum block) 

1–24 °C (silver block) 

Temperature control range of gradient 30–99 °C 

Temperature of lid 105 °C 

Block homogeneity 20–72 °C ±0.3 °C, 

90 °C ±0.4 °C 

Control accuracy ±0.2 °C 

Heating speed* approx. 4 °C/s (aluminum block) 

approx. 6 °C/s (silver block) 

Cooling speed* 

Complete system 

approx. 3 °C/s (aluminum block) 

approx. 4.5 °C/s (silver block) 

Dimensions (W x D x H) 10.24 in x 16.14 in x 15.59 in/26 cm x 41 cm x 39.6 cm 

Total weight 52.91 lb/24 kg 

Voltage requirements 115 V, 50 Hz–60 Hz 

Power consumption 800 W 

* Measured at block 



Mastercycler ep realplex 

Mastercycler ep realplex2 , with aluminum block and two emission filters Please Inquire 

Mastercycler ep realplex2 S, with silver block and two emission filters Please Inquire 

Mastercycler ep realplex4 , with aluminum block and four emission filters Please Inquire 

Mastercycler ep realplex4 S, with silver block and four emission filters Please Inquire 

System accessories 

Real-time PCR consumables, see page 206 or visit www.eppendorf.ca 

Service 

Performance plans, see page 214 for details 

Please contact Eppendorf Canada at 800-263-8715 to receive a quotation for the Mastercycler ep realplex system. 




4 Thermocyclers

4 PCR Plates 


twin.tec® PCR Plates 



4 | Consumables | PCR 

General product features 

‡ One-piece design: combining a polycarbonate frame 

and polypropylene wells for optimum performance 

‡ Extremely thin-walled polypropylene wells guarantee 

optimum heat transfer to the sample 

‡ Exceptionally solid and torque-resistant 

polycarbonate frame 

‡ Improved well-to-well tolerance 

‡ Minimal binding of DNA, RNA and enzymes 

‡ Cut-away corner and alphanumeric grid-referencing 

‡ Raised well rims for effective sealing, also reduces 

risk of cross-contamination 

‡ Autoclavable (121 °C, 20 min) 

‡ Certified free of any detectable human DNA, DNase, 

RNase and PCR inhibitors* 

‡ Available with barcode (upon request) 

‡ Ideal for quantitative real-time PCR as well 

* Certificate, test procedures and detailed information available on request. 

Additional features of twin.tec PCR Plate 96, 

skirted and semi-skirted 

‡ Compatible with automated systems 

‡ Skirted for label or bar code 

‡ Fits most thermal cyclers and the MegaBACE ® 

capillary sequencer 

‡ Within SBS footprint recommendations 

(127.76 x 85.47 mm ±0.25 mm) 

‡ Stackable 

‡ Eight holes in the skirt aid plate positioning and removal 

from the thermal cycler block 

‡ Low profile design enhances efficiency of PCR and 

enables the highest efficiency for small sample volumes 

‡ 150 μL max. well volume (when used with cap strips, skirted) 

‡ 250 μL max. well volume (when used with cap strips, 

semi-skirted) 

Additional features of twin.tec PCR Plate 384 

‡ Continuous skirt for compatibility with 

automated systems 

‡ Skirted for label or bar code 

‡ Stackable 

‡ Within SBS footprint recommendations 

‡ Ideal for most common 384-well thermal cyclers, 

particularly for Eppendorf Mastercycler ® pro 384 

(see page 189) 

‡ 45 μL max. well volume 

For more information visit www.eppendorf.ca/twintecplates



Product features of twin.tec PCR Plate 96, unskirted 

‡ Fits on a wide variety of thermal cyclers 

‡ OptiTrack ® matrix: high contrast labeling of alphanumeric 

grid for easier sample tracking 

‡ Available with regular (250 μL) or low profile (150 μL) wells 




Product features of twin.tec PCR Plate 96, unskirted, 

divisible 

‡ Can be divided in 4 segments of 24 wells each 

‡ Fits on a variety of thermal cyclers 

‡ OptiTrack matrix: high contrast labeling of alphanumeric 

grid for easier sample tracking 

‡ Available with regular (250 μL) or low profile (150 μL) wells 

Product features of twin.tec microbiology PCR Plate 

‡ Sterile, individually blistered plates 

‡ Additionally free of bacterial DNA 

‡ For all applications that need ultimate purity or certified 

absence of bacterial DNA 

4 PCR Plates

4 PCR Plates 



Technical specifications. All dimensions in millimeters. 






twin.tec PCR Plate 96*, skirted (clear wells), 25 pcs., (150 μL max. volume) 

Clear frame 951020401 

Yellow frame 951020427 

Green frame 951020443 

Blue frame 951020460 

Red frame 

twin.tec PCR Plate 96*, skirted (black wells), 25 pcs., (150 μL max. volume) 

951020486 

Yellow frame 

twin.tec PCR Plate 96*, semi-skirted (colorless wells), 25 pcs., (250 μL max. volume) 

951020508 





Red frame 

twin.tec PCR Plate 384* (colorless wells), 25 pcs., (45 μL max. volume) 

951020389 





Red frame 

twin.tec PCR Plate 96, unskirted low profile, 20 pcs., (150 μL max. volume) 

951020745 





Red frame 0030133340 

Clear frame, divisible 0030133358 

Blue frame, divisible 

twin.tec PCR Plate 96, unskirted, 250 µL, 20 pcs., (250 μL max. volume) 

0030133382 



Clear frame, divisible 0030133374 

Blue frame, divisible 

twin.tec microbiology PCR Plate 96, skirted, 10 pcs., (150 μL max. volume) 

0030133404 


Blue frame 

twin.tec microbiology PCR Plate 96, semi-skirted, 10 pcs., (250 μL max. volume) 

0030129318 


Blue frame 

twin.tec microbiology PCR Plate 384, 10 pcs., (45 μL max. volume) 

0030129334 



* Eppendorf owns protective rights under European Patent EP 1 161 994 and US patents US 7,347,977 and US 6,340,589 

For more information on Eppendorf purity levels, see pages 394 to 397, or visit www.eppendorf.ca/purity. 




4 PCR Plates

4 PCR Tubes 


PCR Tubes 

More space for labeling due to improved lid design 

Fig. 1: 0.5 mL PCR Tube 

writing surface 

Product features of 0.5 mL PCR Tubes, thin-walled Product features of 0.2 mL PCR Tubes, thin-walled 

‡ Special space-saving lid design 

‡ For maximum capacity in the universal blocks of 

Eppendorf Mastercyclers ® and other 0.5 mL blocks 

‡ High transparency 

‡ Etched lid for labeling 

‡ Tight sealing 

‡ Easy to open 

‡ For use with all thermal cyclers with 0.5 mL block format 

‡ Certified free from human DNA, DNase, RNase and 

PCR inhibitors* 

Description 

Original Eppendorf ® 0.2 mL and 0.5 mL PCR Tubes are 

manufactured to the highest quality standards. These thin-walled 

polypropylene tubes ensure efficient and homogeneous heat 

transfer of the sample, thanks to their even wall thickness and 

smooth wall surface. Heat-sealed bags of tubes are enclosed in 

boxes that are also sealed with a PCR clean sticker to ensure the 

highest degree of purity. 



Contamination shield prevents contact with inner lid surface 

Fig. 2: 0.2 mL PCR Tube 

writing surface 

‡ Contamination shield on hinged lid 

‡ Defined lid position due to special hinge 

‡ High transparency even on the tube base 

‡ Etched lid for labeling 

‡ Tight sealing 

‡ Easy to open 

‡ For use with all thermal cyclers with 0.2 mL block format 

‡ Also available in 8-tube strip format 

‡ Certified free from human DNA, DNase, RNase and 



defined lid position due 

to special hinge

PCR Tubes 


Product features of 0.1 mL PCR Tube Strips 

‡ 8 reaction tubes in strip-format —ideal for small 

sample volumes 

‡ Easily adaptable for automation 

‡ Sealable using flat or domed strip caps 

‡ Certified free from human DNA, DNase, RNase 

and PCR inhibitors* 


Description 

0.1 mL Eppendorf 

Catalog No. 

® PCR Tubes 

PCR Tube Strips 0.1 mL, without lids (10 × 12 strips) 0030124804 

PCR Tube Strips 0.1 mL plus Cap Strips, domed (10 × 12 strips each) 0030124812 

PCR Tube Strips 0.1 mL plus Cap Strips, flat (10 × 12 strips each) 

0.2 mL Eppendorf PCR Tubes 

0030124820 

Clear, 1,000 pcs. 951010006 

8-tube strip, for 0.2 mL PCR Tubes, pack of 120 (960 tubes total), with dome hinged cap 

0.5 mL Eppendorf PCR Tubes, thin-walled with hinged lid 

951010022 

Clear, 500 pcs. 0030124537 




4 PCR Tubes

4 PCR Plates 


twin.tec® real-time PCR Plates 


‡ White wells for better reflection 

‡ High mechanical stability makes them torque-resistant 

‡ Raised rims for effective sealing 

‡ “Skirted” (stackable) and “semi-skirted” plates 

‡ Optimal heat transfer due to reduced wall thickness 


‡ Certified free of any detectable human DNA, DNase, 

RNase and PCR inhibitors* 

‡ Available with barcode (upon request) 


Reflection is up to 10-fold higher than with frosted wells. 



Description 

Eppendorf ® twin.tec real-time PCR Plates 1 offer all the advantages 

of regular twin.tec plates and give you the advantage of white 

wells for your real-time PCR. The limiting factor in low volume 

real-time PCR is often the remaining intensity of fluorescence. 

The white wells of the Eppendorf twin.tec real-time PCR plates 

reflect fluorescence much better than clear or frosted wells. 

Thus, lower levels of fluorescence are still detectable with 

the same instrument—just by using the right consumables. 

Additionally, white wells significantly reduce interfering 

background fluorescence and lead to increased homogeneity 

of replicates and reproducible results. The rigidity of the 

polycarbonate frame ensure easy and reliable handling— 

manually or automated. The polypropylene wells guarantee 

excellent and fast temperature transfer to the sample. 

1 Eppendorf owns protective rights under European Patent EP 1 161 994 and US patents US 7,347,977 

and US 6,340,589 

For more information visit www.eppendorf.ca/realtime

twin.tec® real-time PCR Plates 


Description 

twin.tec 96 real-time PCR Plates, skirted, set of 25 pcs. 

Catalog No. 

White frame 951022015 


Black frame 

twin.tec 96 real-time PCR Plates, semi-skirted, set of 25 pcs. 

951022027 



Black frame 

twin.tec 96 real-time PCR Plates, unskirted low profile, set of 20 pcs. (150 μL max. volume) 

951022067 



Black frame 

twin.tec 384 real-time PCR Plates, skirted, set of 25 pcs. 

0030132726 



Black frame 0030132750 

For more information on Eppendorf purity levels, see pages 

394 to 397, or visit www.eppendorf.ca/purity. 

Masterclear® Cap Strips and real-time PCR Tube Strips 



Masterclear Cap Strips 


‡ Inverted dome to avoid scratching of the optical surface 

‡ Inverted dome to reduce dead volume of the tubes 

‡ Optimized for ultimate light transmission 

‡ Optically clear for real-time PCR 

real-time PCR Tube Strips 

‡ White wells for better reflection—ideal for use with lower 

sample volume 

‡ High mechanical stability 

‡ Extremely thin walls for optimal heat transfer 

‡ 0.1 mL Tube Strips 


Masterclear Cap Strips, 10 x 12 pieces 951022089 

real-time PCR Tube Strips, without caps, 10 x 12 pieces 951022102 

Masterclear Cap Strip and real-time PCR Tube Strip, 10 x 12 pieces each 951022109 



4 PCR Plates

4 Heat Sealing Materials 


Heat Sealer 

Heat sealing materials 


‡ Hermetic heat sealing of multiwell plates 

‡ Eliminates evaporation in PCR, reducing 

cross-contamination 

‡ Ideal for sample transportation and storage 

‡ Seals 96- and 384-well plates 

‡ Suitable for plates of different heights 

‡ Optimum sealing with Eppendorf ® Heat Sealing Foils 

and Films at a preset temperature 

‡ Compact design, portable device 

‡ Integrated thermostat prevents overheating 

‡ Heating plate faces away for safety 

‡ Improved mechanics for even easier sealing 






Heat Sealer, 115 V/50 Hz 951023078 

Base plate, for 384-well plate 951023086 

‡ Hermetic sealing of multiwell plates, especially 

recommended for low reaction volumes 

‡ Best protection against evaporation during PCR 

‡ Certified free of human DNA, DNase, RNase and 


‡ PCR foil is both pierceable and peelable 

‡ Maximum adhesiveness at high temperature during PCR 

* Certificate, test procedures and detailed information available upon request. 



Heat Sealing PCR Film, 100 pcs. 0030127838 

Heat Sealing PCR Foil, 100 pcs. 0030127854

Heat sealing materials 


Description Heat Sealing PCR Film Heat Sealing PCR Foil 

Packaging unit 1 x 100 pcs. 1 x 100 pcs. 

Features 

‡ Optically clear polyester/ 

polypropylene laminate 

‡ Extremely stable sealing option — 

cannot be removed or pierced 

‡ Laminated aluminium foil 

‡ Easily removable 

‡ Easily pierceable, even with 

multichannel pipettes 


Seal integrity –80 °C to 100 °C –80 °C to 100 °C 

Sealing time with 

Eppendorf ® Heat Sealer 

4 sec. 4 sec. 

Weldable materials 

‡ Polypropylene 

‡ Polypropylene 

Special applications 

‡ Colorimetric applications 

‡ Recommended for use in automated 

‡ Fluorescence applications, 

including real-time PCR 

‡ Storage of hazardous samples 

work processes 

PCR Film (self-adhesive) and PCR Foil (self-adhesive) 


‡ Effective adhesive sealing prevents evaporation loss 

during reaction and storage 

‡ Removal from the plate with no residue 



PCR Film 

‡ Sample can be visually monitored through the 

transparent film 

PCR Foil 

‡ Easily pierced 

‡ No sticking of the pipette tip (ideal for automated systems) 

* Certificate, test procedures and detailed information available upon request. 



PCR Film (self-adhesive), 0030127811 

100 pcs. 

PCR Foil (self-adhesive), 0030127820 

100 pcs. 



4 Heat Sealing Materials

4 Heat Sealing Materials 


Masterclear® real-time PCR Film 

Cap Strips 

Capping Aid 





‡ Optimized for maximum light transmission 

‡ Effectively forms a tight seal to reduce evaporation 

‡ Maximum handling comfort due to optimized packaging 

‡ Optically clear for real-time PCR 



Masterclear real-time PCR Film, 951022115 

(self-adhesive), 100 pieces 


‡ Strips with eight microcaps for 0.1 and 0.2 mL wells 

‡ Easy and rapid sealing of Eppendorf ® twin.tec ® PCR 

Plates, PCR strips and other standard multiwell plates 

‡ Flat Cap Strips are suitable for real-time PCR 




Cap Strips (8-strips), 

0030124839 

domed, 10 x 12 pcs. 

Cap Strips (8-strips), 

0030124847 

flat, 10 x 12 pcs. 


‡ Tool for easy and fast sealing of 0.2 mL PCR test tubes 

and twin.tec PCR Plates with Cap Strips 

‡ Several wells and tubes can be sealed simultaneously 

‡ Minimal force required for an effective seal 

‡ Minimizes losses due to evaporation 





Capping Aid, 1 pc. 951023108 

* Certificate, test procedures and detailed information available upon request.

Work tray and frame 

PCR Rack 



‡ Frame with work tray serves as a 2-part polycarbonate 

rack for 96 x 0.2 mL PCR tubes 

‡ All tubes can be directly transferred to a thermoblock 








Work tray, for 96 x 0.2 mL 

PCR tubes, set of 10 

951010031 

Frame for work tray, set of 5 951010049 

‡ Rack for handling and storing tubes and plates 

‡ For 0.2 mL, 0.3 mL and 0.5 mL tubes, as well as 

96-well plates 

‡ Stackable 

‡ Can be stored down to –90 °C 




PCR Rack, 10 pcs. 0030124545 

4 Heat Sealing Materials

4 Adapters 


in situ Adapter 


Sample capacity Max. 4 glass slides 76 x 26 mm 

Temperature range 4–99 °C 

Temperature distribution 20–72 °C ±0.6 °C 

across the adapter 

95 °C 

±1.0 °C 

Temperature control speed ≥1.0 °C/s (heating) 

measured on the adapter ≥0.7 °C/s (cooling) 

Temperature Validation System for Mastercycler Family 






Temperature Validation System 

(96-well probe included) 

950008059 

Temperature sensor, 384-well 950008041 


‡ Easy to handle 

‡ Perfect fit for optimal heat transfer 

‡ Software supported, easy to program, extremely flexible 

‡ Can accommodate 4 standard glass slides 

‡ Transparent covering hood—in situ frames trap moisture 

(sold separately) 

Description 

The in situ Adapter can be used to convert your Mastercycler ® 

or Mastercycler nexus to an in situ device as easily as if you 

were to insert a PCR plate. And the quality of the heat transfer 

is equally as high! With precise block homogeneity, the in situ 

Adapter has room for up to four standard glass slides. 



in situ Adapter 

950007052 

for Mastercycler nexus 

and Mastercycler 

in situ Frames Please Inquire 


‡ Single-well probe 

‡ Sensor for 0.2 mL tube position 

‡ Optional: sensor for 384-well block, sold separately 

‡ Simple, rapid validation and calibration 

‡ Autocalibration with Mastercycler software, version 

2.10 or higher 

‡ Can be third-party calibrated 

Description 

The Temperature Validation System for the entire Mastercycler, 

Mastercycler nexus, Mastercycler ep and Mastercycler pro 

families enables simple and rapid validation and calibration of 

block temperature. The program for automatic measurement 

and calibration is integrated into the thermal cycler software, 

thus providing an accurate and precise system that meets all 

documentation and detection requirements in the laboratory.

Temperature Verification System 


‡ Verification of the temperature control of the thermoblock 

and the heated lid in accordance with the appropriate 

Eppendorf ® specifications 

‡ Generation of certificates of conformity and of measuring 

reports for the thermocyclers 

‡ The temperature control of the thermoblock can be 

adjusted automatically, if necessary 

‡ Recording of a temperature profile of user defined 

programs in 14 thermoblock positions 

‡ Increased number of measuring points: 14 block 

thermosensors and 1 lid thermosensor 

‡ Temperatures of all positions are measured 

simultaneously—short data acquisition time per cycler 


Description 

Temperature Verification System 

Catalog No. 

includes 96-well sensor plate 6328000006 

includes 384-well sensor plate 6328000014 

Sensor plate for Temperature Verification System 

96-well 6328071000 

384-well 6328072007 





Description 

The Eppendorf Temperature Verification System can 

perform a verification, and if necessary an adjustment, 

of the Mastercycler ® pro, Mastercycler ep, Mastercycler nexus 

and Mastercycler ep realplex instruments according to the 

manufacturer’s specifications. This system can also record 

temperature profiles and measure ramp rates to ensure that 

the thermal cycler is always at its peak performance level. 

The Eppendorf Temperature Verification System has 14 

block thermosensors and 1 lid thermosensor, all positions 

are measured simultaneously for rapid data acquisition. 

4 Thermocycler Software

Services – PCR 

44 

214 Sample Service products Handling 

Mastercycler® ep realplex Performance Plans and Services 

Value of Service 

From warranty plus Protection that provides a LED light source 

for life and a 2-year system warranty, to service plans for 

every lab need, our goal is to help you achieve reproducible 

results over an extended period of time. Reliable quantification 

is critical in qPCR and Eppendorf ® Service will keep your 

instrument in peak performance. 

Choose a plan that’s right for you and a certified Eppendorf 

Specialist professional will handle it all. Extended Warranty 

and Advantage Service protect your hardware investment 

while any one of three Performance Plans will keep your 

system fine tuned and in compliance to deliver results you 

can count on. 

Service offers—ep realplex 

Performance Plans—ep realplex 



Long-term benefits of performance plans: 

‡ Confidence in results 

‡ GLP compliance 

‡ Assurance of performance over extended lifetime 

Features of Eppendorf Performance Plans: 

‡ Complete satisfaction and quality delivered by 

Eppendorf experts 

‡ Complete programs—easy to understand, 

easy to budget, no surprises 

‡ Complete documentation management— 

everything you need to be in compliance 

Description Model Catalog No. 

Extended Warranty Mastercycler ep realplex 2-color system 950020927 

Mastercycler ep realplex 4-color system 950020935 

Advantage Repair Service Mastercycler ep realplex 2-color system 901520068 

Preventive 

Certification 

Description 

Maintenance Certification 

Plus 

Catalog No. 950033017 950033025 950033037 

Preventive Maintenance 

The Preventive Maintenance is carefully designed to keep 

the Mastercycler ep realplex in the best condition. The 

services include the following—cleaning of the instrument’s 

surface and block, a functional test of the thermoblock with 

a temperature validation, as well as a full system check with 

background calibration. 

Color Calibration 

A dedicated color calibration protocol is carried out by the 

service specialist. Based on this recalibration procedure the 

last issued certificate is updated. 

Validation Assay 

The validation assay is based on a qPCR run with 

specifically designed primers and DNA templates to cover 

all major performance aspects such as dynamic range and 

repro ducibility. Based on this validation protocol the last 

issued certificate is updated. 

Mastercycler ep realplex 4-color system 901520070 

‡ ‡ ‡ 

— ‡ ‡ 

— — ‡

Mastercycler® Systems Services 

Value of Service 

From a 2-year system warranty that comes with the unit, 

to Extended Warranties and Preventive Maintenance, we 

offer options that will keep your Mastercycler running for 

many cycles. 

Choose an Extended Warranty or Advantage Service to 

ensure optimal performance of your instrument while 

Preventive Maintenance will keep the system running 

smoothly for years to come. 

Advantage Repair Service 

An easy to use value packaged service option when a 

repair is needed for systems not covered under a warranty 

or extended warranty. All it takes is one call to our Service 

Center and for a single fee we will repair the instrument, 

complete a full preventive maintenance, repair anything 

else we find, plus include a full year warranty on the whole 

system, not just the repair. Instrument return shipment is 

also included. 

Service offers—Mastercycler systems 


Extended Warranty Mastercycler personal 901510551 

Mastercycler 901510553 

Mastercycler gradient 901510555 

Mastercycler ep/pro Manual lid 901510556 

Mastercycler ep Motorized lid 901510557 

Mastercycler nexus Please Inquire 

Advantage Repair Service Mastercycler 901520044 

Mastercycler gradient 901520046 

Mastercycler personal 901520048 

Mastercycler ep/pro Manual lid 901520050 

Mastercycler ep Motorized lid 901520052 

Mastercycler ep/pro S Manual lid 901520054 

Mastercycler ep S Motorized lid 901520056 

Mastercycler ep/pro 384 Manual lid 901520058 

Mastercycler ep 384 Motorized lid 901520060 

Mastercycler ep/pro Control Panel 901520061 

Performance Plans—Mastercycler Mastercycler systemsnexus 

Please Inquire 


Preventive Maintenance Mastercycler Systems 901510526 

Temperature Validation 

and Calibration 

Mastercycler Systems 901510697 

Sample Service products Handling 215 



4 Services – PCR

Support and Services Directory 

Contact Information 

United States Canada 

Business Hours: 8:30 a.m. to 8:00 p.m. EST 8:30 a.m. to 5:00 p.m. EST 

Phone: 800-645-3050 

516-334-7500 

800-263-8715 

905-826-5525 

Fax: 516-334-7506 905-826-5424 

Website: www.eppendorfna.com www.eppendorf.ca 

Email: info@eppendorf.com canada@eppendorf.com 

Customer Support: 800-645-3050 

custserv@eppendorf.com 

Technical Service: 800-645-3050 

techserv@eppendorf.com 

www.eppendorf.com • Email: info@eppendorf.com 

800-263-8715 

canadacustserv@eppendorf.com 

800-645-3050 

techserv@eppendorf.com 

In the U.S.: Eppendorf North America, Inc. 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715

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