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4 A History of Advanced PCR<br />
184 Sample Handling<br />
Quality is timeless<br />
<strong>Eppendorf</strong> ® is an innovator, designing instruments that<br />
fit the needs of today’s scientist. The Mastercycler ®<br />
gradient by <strong>Eppendorf</strong>—still popular today—was<br />
the first available gradient thermal cycler. Today the<br />
gradient function remains critical for the optimization<br />
of a PCR reaction.<br />
The next generation Mastercycler ep is a lab favorite<br />
and still one of the fastest cyclers around. Given this<br />
history of innovation, it’s only natural that <strong>Eppendorf</strong><br />
would now introduce the latest in evaporation reduction<br />
technology—vapo.protect ® . With a shift to low volume<br />
reactions and faster protocols, the need to safeguard<br />
the integrity of your sample has become increasingly<br />
vital. The Mastercycler pro does just that.<br />
Survival of the Fittest—<br />
In the age of networking, the new Mastercycler nexus is<br />
your reliable companion when it comes to daily routine<br />
PCR. With its ease-of-use, small footprint, energy saving<br />
features and its’ capability to notify you by e-mail when it<br />
is finished—what else do you need from a cycler?<br />
<strong>Eppendorf</strong> has also addressed the needs of real-time<br />
PCR. Integrating the advantages of the Mastercycler ep<br />
gradient family, we have developed a fully licensed<br />
real-time PCR system that meets the requirements<br />
of the latest quantitative PCR applications.<br />
A history of advanced PCR<br />
‡ Microcycler: <strong>Eppendorf</strong><br />
introduces its first thermal<br />
cycler using water to heat<br />
and cool.<br />
‡ Mastercycler 5330:<br />
<strong>Eppendorf</strong> introduces the<br />
first Mastercycler based<br />
on peltier technology.<br />
1990 1993<br />
Faster speeds, highest precision, excellent temperature<br />
control stability, extremely user-friendly, and absolute<br />
reliability united with a flexible concept: these are the<br />
characteristics of the Mastercycler families.<br />
‡ Mastercycler gradient:<br />
the first gradient thermal<br />
cycler on the market.<br />
‡ Thin-walled 0.2 mL<br />
PCR tubes allow for<br />
rapid heat transfer.<br />
‡ Dual component twin.tec ®<br />
plates provide a stable<br />
frame and extremely<br />
thin-walled wells for<br />
optimum heat transfer.<br />
1997 1998 2002
‡ Mastercycler ep:<br />
Fast heating/cooling rates<br />
and ultra quiet operation—<br />
in a compact design.<br />
2003 2005<br />
The Mastercycler® Family<br />
‡ Mastercycler ep<br />
realplex: Extremely<br />
fast optics for rapid<br />
data acquisition.<br />
Sample Handling 185<br />
Then... Now—always providing quality solutions for your lab.<br />
‡ Mastercycler pro:<br />
New vapo.protect ®<br />
technology reduces<br />
evaporation. ‡ qPCR consumables<br />
and divisible twin.tec<br />
PCR plates.<br />
‡ Mastercycler nexus:<br />
Connects to network<br />
to send status updates<br />
through e-mail.<br />
2008 2010 Today<br />
4 A History of Advanced PCR
4 Summary of Applications<br />
186 Sample Handling<br />
Summary of applications<br />
Application<br />
Instruments<br />
Product Page<br />
Conventional PCR Mastercycler ® pro<br />
188<br />
Mastercycler nexus Family<br />
192<br />
Real-time PCR Mastercycler ep realplex 195<br />
PCR optimization Mastercycler pro<br />
188<br />
Mastercycler nexus gradient<br />
194<br />
in situ PCR Mastercycler nexus<br />
192<br />
Mastercycler nexus gradient with in situ Adapter 212<br />
Mastercycler nexus flat<br />
194<br />
Cycle Sequencing Mastercycler pro<br />
188<br />
Mastercycler nexus Family<br />
192<br />
Heat sealing of 96- and 384-well PCR plates<br />
Consumables<br />
Tubes, Plates and Accessories<br />
Heat Sealer 208<br />
PCR with sample throughput 0.2/0.5 mL PCR Tube, thin-walled 204<br />
PCR with medium sample throughput 0.2 mL PCR Tubes in strips of 8<br />
0.1 mL PCR Tubes in strips of 8<br />
twin.tec ® 205<br />
205<br />
PCR Plate 96, unskirted, divisible 201<br />
PCR with medium to high sample throughput twin.tec PCR Plate 96, semi-skirted<br />
200<br />
twin.tec PCR Plate 96, skirted<br />
200<br />
PCR with medium to high sample throughput,<br />
usage in automatic devices<br />
twin.tec PCR Plate 96, skirted 200<br />
PCR with high sample throughput,<br />
twin.tec PCR Plate 384<br />
200<br />
usage in automatic devices<br />
twin.tec real time PCR Plate 384<br />
206<br />
Real-time PCR twin.tec real-time PCR Plates<br />
206<br />
real-time PCR Tube Strips<br />
207<br />
PCR with bacterial templates<br />
Accessories<br />
twin.tec microbiology PCR Plate 201<br />
Non-cooled PCR Rack, work tray and frame 211<br />
Cooled (0 °C and –21 °C) IsoTherm-System ® 124<br />
Cooled (0 °C), color change for temperature control<br />
Sealing options<br />
PCR-Cooler 125<br />
Partial seal for real-time PCR and low volume PCR Masterclear ® Cap Strips 207<br />
Partial seal of PCR plates and PCR strips Cap Strips, Capping Aid 210<br />
Heat sealing, transparent, non-removable,<br />
cannot be pierced (e.g. for colorimetric evaluation)<br />
Heat Sealing Film 208<br />
Heat sealing, non-transparent, removable,<br />
can be pierced<br />
Heat Sealing Foil 208<br />
Adhesive seal, transparent, removable,<br />
cannot be pierced<br />
PCR Film 209<br />
Adhesive seal, transparent, removable,<br />
cannot be pierced<br />
PCR Foil 209<br />
Adhesive seal, transparent, optically clear, removable, cannot be pierced Masterclear real-time PCR Film 210<br />
4 | Info | PCR<br />
Practice of the patented polymerase chain reaction (PCR) process requires a license. The Mastercycler is an Authorized Thermal Cycler and<br />
may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in<br />
accordance with the label rights accompanying such reagents.<br />
This product is sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc. and Applied Biosystems.<br />
Product appearance, specifications, and/or prices are subject to change without notice.
Summary of applications<br />
Sample Handling 187<br />
Application Product Page<br />
Application tips for PCR<br />
The table to the left shows a selection of particular applications from the<br />
PCR area and serves as an overview. The complete product portfolio of<br />
the <strong>Eppendorf</strong> ® PCR System is found on the following pages.<br />
A selection of particular PCR products shown on the following pages, can<br />
be provided as Customized Solutions. Further information on page 380.<br />
Register your <strong>Eppendorf</strong> products online now to benefit from numerous<br />
advantages! You receive bonus ep-points immediately for each <strong>Eppendorf</strong><br />
device registered. You can also easily manage your devices online and are<br />
always kept up-to-date on any news.<br />
epGreen ® Initiative<br />
‡ Noise emission: <strong>Eppendorf</strong> PCR instruments are optimized<br />
to minimize noise emission<br />
‡ PCR consumables are made of virgin Polypropylene and<br />
Polycarbonate that is fully recyclable<br />
‡ All cardboard packaging is 100 % recyclable<br />
‡ Wherever possible, water-soluble laquer or paints without<br />
heavy metals are used<br />
‡ Mini-Satellite-system: By controlling up to 5 units of the<br />
Mastercycler ® pro with only 1 Control Panel, you can<br />
increase your PCR capacity by only increasing the parts<br />
needed, i.e. the thermo module. There is no need to buy<br />
an entire additional system to increase your capacity<br />
Mastercycler nexus, Mastercycler nexus gradient,<br />
Mastercycler nexus eco<br />
Purchase of this instrument conveys a limited non-transferable<br />
immunity from suit for the purchaser’s own use under certain<br />
non real-time thermal cycler patents licensed from Applied<br />
Biosystems, LLC.<br />
Mastercycler ep realplex<br />
This is a Licensed Real-Time Thermal Cycler or Licensed<br />
Real-Time Temperature Cycling Instrument under Applera’s<br />
United States Patent No. 6,814,934 and corresponding claims<br />
in non-U.S. counterparts thereof, for use in research and for all<br />
other applied fields except human in vitro diagnostics. No right<br />
is conveyed expressly, by implication or by estoppel under any<br />
other patent claim.<br />
customized<br />
products<br />
p. 380<br />
‡ Connect up to 2 Mastercycler nexus eco units to your<br />
existing unit to acheive low energy consumption<br />
‡ Stand-by function of the Mastercycler nexus saves energy<br />
For more information on epGreen, see pages 398 and 399.<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
Summary of Applications<br />
4
Thermocyclers<br />
4<br />
188 Sample Handling<br />
Mastercycler® pro<br />
®<br />
vapo.protect ® technology*<br />
Evaporation in a PCR reaction vessel not only limits the ability<br />
to work with low reaction volumes but also makes it difficult to<br />
obtain reproducible results. For the Mastercycler pro it has been<br />
<strong>Eppendorf</strong>’s aim to protect the PCR reaction from evaporation.<br />
While common PCR instruments press a metal plate onto<br />
the PCR consumable, the vapo.protect lid covers the PCR<br />
consumable with a cushion that conforms to the shape of the<br />
consumable—no matter which consumable you use. The snug<br />
fit of the cushion in combination with the high pressure and<br />
excellent heating capabilities of the lid reduce evaporation to<br />
a minimum.<br />
Evaporation leads to increasing concentrations of the PCR<br />
master mix components, especially primers. This, in turn,<br />
leads to non-specific binding. The changes in concentrations<br />
due to evaporation both during the experiment and between<br />
experiments can be a cause for great concern for any scientist<br />
who tries to keep variables to a minimum.<br />
Gradient with SteadySlope ® technology<br />
The gradient option of the Mastercycler pro features<br />
SteadySlope technology to ensure that the heating and<br />
cooling ramp rates are identical in both gradient and normal<br />
operation. Thus, you can expect identical temperature control<br />
characteristics during both optimization and routine experiments.<br />
Programming the gradient with the Control Panel or the<br />
CycleManager pro is easy and intuitive—so that novice users<br />
can get started quickly and safely.<br />
*Patents pending<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
vapo.protect<br />
technology leads to improved<br />
reproducibility and specificity<br />
at the rim and in the corners<br />
of the block<br />
Certified Quality<br />
– Individual, documented quality control certificate<br />
– Calibration according to national and international standards:<br />
NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK)<br />
4 | Instruments | PCR<br />
Description<br />
Fastest speeds, highest precision, easy to use and absolute<br />
reliability combined in a flexible concept—that is the<br />
Mastercycler pro.<br />
The requirements for PCR are defined by research projects that<br />
are continually evolving. As the needs of the researchers change<br />
the instrument they use needs to be adaptable. That is why only<br />
an open and flexible system fits into today’s lab. <strong>Eppendorf</strong>’s<br />
Mastercycler pro offers ultimate flexibility:<br />
– choose from three block formats: 96, 96 Silver and 384<br />
– operate the Mastercycler pro in various configurations<br />
– use any plate, tube or strip that you choose<br />
Unsurpassed speed can be achieved with <strong>Eppendorf</strong>’s Impulse<br />
PCR, a device driven hot-start function that increases heating<br />
rates to 8 °C/s.<br />
For additional information, see application tips for PCR<br />
on page 393.
Mastercycler® pro<br />
Technical specifications<br />
Sample Handling 189<br />
Description Mastercycler pro Mastercycler pro S Mastercycler pro 384<br />
Thermoblock Aluminum Silver Aluminum<br />
Sample capacity 96 x 0.2 mL PCR tubes or 1 PCR plate 8 x 12 (unskirted,<br />
semi-skirted, skirted—according to SBS standard)<br />
Temperature control range of the block 4–99 °C 4–99 °C 4–99 °C<br />
1 PCR plate 384<br />
Temperature control mode Fast, Standard, Safe ; all available in gradient mode<br />
Heating technology of the block Peltier Elements, Triple Circuit Technology<br />
Gradient block Over 12 columns Over 12 columns Over 24 columns<br />
Gradient range 1–20 °C 1–24 °C 1–20 °C<br />
Gradient temperature range 30–99 °C 30–99 °C 30–99 °C<br />
Lid temperature range 37–110 °C 37–110 °C 37–110 °C<br />
Lid descent and closing pressure vapo.protect ® technology, with Thermal Sample Protection<br />
Block homogeneity 20–72 °C ±0.3 °C,<br />
90 °C ±0.4 °C<br />
Control accuracy ±0.2 °C ±0.2 °C ±0.2 °C<br />
Heating rate* approx. 4 °C/s approx. 6 °C/s approx. 4 °C/s<br />
Cooling rate* approx. 3 °C/s approx. 4.5 °C/s approx. 3 °C/s<br />
Interfaces 1 x Centronics ® , 1 x RS-232, Control panel, one each of CAN_in/CAN_out<br />
Dimensions (W x D x H) 10.2 x 16.3 x 14.6 in/<br />
26 x 41.5 x 37 cm<br />
Product features<br />
‡ Extremely fast heating and cooling rates—<br />
get results faster and do more in one day<br />
‡ Ultimate reduction of evaporation for reliable results<br />
‡ Gradient blocks with SteadySlope ® technology for<br />
protocol optimization<br />
‡ Intuitive graphic user interface<br />
‡ Display to indicate cycler number in a network<br />
‡ Upgradable to real-time PCR<br />
‡ 2-year warranty<br />
‡ Optional self-test dongle available to test functionality<br />
of the Peltier Elements<br />
‡ Stand alone system fits on virtually every lab bench<br />
and is operated by a control panel<br />
‡ Expandable to a Mini Satellite System or network<br />
– Mini Satellite System: operated by Control Panel.<br />
Control up to 5 units with one Control Panel.<br />
Combine any block format. Expandable to a network.<br />
– Computer Network: combines up to 30 units for<br />
ultimate throughput. Operated by a computer and the<br />
CycleManager pro software. Benefit from a client/<br />
server architecture and full software functionality.<br />
10.2 x 16.3 x 14.6 in/<br />
26 x 41.5 x 37 cm<br />
10.2 x 16.3 x 14.6 in/<br />
26 x 41.5 x 37 cm<br />
Weight 40.8 lbs /18.5 kg 40.8 lbs /18.5 kg 40.8 lbs /18.5 kg<br />
Power supply 120 V, 50–60 Hz 120 V, 50–60 Hz 120 V, 50–60 Hz<br />
Power consumption 950 W 950 W 950 W<br />
* Block temperature<br />
This product is licensed under U.S. patent Nos. 5,525,300, 5,779,981 and 6,054,263. The heated cover device is licensed under US 5,552,580 and foreign equivalents. The user of the <strong>Eppendorf</strong> Mastercycler pro<br />
might require additional rights for kits, reagents and other components required for his/her application. Such accompanying rights for these kits, reagents and other may be obtained by the respective holder<br />
of such rights. No rights are conveyed expressly, by implication or estoppel to any patents on real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent or kit.<br />
Mastercycler pro upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts.<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 Thermocyclers
Thermocyclers<br />
4<br />
190 Sample Handling<br />
Mastercycler® pro<br />
Control panel programming features<br />
‡ Highest degree of flexibility, graphical user interface<br />
‡ Variable ramp rates<br />
‡ Simulation mode for temperature control speed and<br />
protocols of the original Mastercycler family—<br />
heating 3 °C/s, cooling 2 °C/s<br />
‡ Time and temperature increments<br />
‡ Pause command<br />
‡ Programmable alarm sound<br />
‡ Freely configurable auto-restart dialogue<br />
Regulatory documentation<br />
Compliance with GMP/GLP guidelines is becoming increasingly<br />
important, particularly in the pharmaceutical and biotechnology<br />
industries. The Mastercycler pro system’s programming and user<br />
management system provides a GLP-conforming environment<br />
for your PCR assays.<br />
Technical specifications of the Control Panel<br />
Number of Mastercycler pro cyclers that can be controlled 1–5<br />
Memory capacity 16 MB, >700 programs<br />
Memory expansion MultiMediaCard reader and 2 x USB port 2.0<br />
Number of user-defined folders >100<br />
Dimensions (W x D x H) 10.0 x 6.6 x 4.4 in/25 x 16.5 x 11 cm<br />
Interfaces 2 x USB port<br />
Weight 2.6 lb/1.2 kg<br />
Power supply, consumption Via the connected thermal module<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
Program and user administration features<br />
‡ One administrator to manage user and service functions<br />
‡ Easy addition of new users with limited rights<br />
‡ Ability for Mastercycler pro users to change their<br />
passwords; no administrator access to user passwords<br />
‡ Password protection against unauthorized login<br />
‡ A report file for each run, including total temperature<br />
profiling<br />
‡ Development, testing and validation of the software<br />
using certified standards<br />
This product is licensed under U.S. patent Nos. 5,525,300, 5,779,981 and 6,054,263. The heated cover device is licensed under US 5,552,580 and foreign equivalents. The user of the <strong>Eppendorf</strong> Mastercycler pro<br />
might require additional rights for kits, reagents and other components required for his/her application. Such accompanying rights for these kits, reagents and other may be obtained by the respective holder of such<br />
rights. No rights are conveyed expressly, by implication or estoppel to any patents on real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent or kit. Mastercycler pro<br />
upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts.
CycleManager pro for Mastercycler® pro<br />
Ordering information<br />
Product features<br />
Sample Handling 191<br />
‡ Network and control up to 30 Mastercycler pro<br />
thermal cyclers with a PC; mix and match models<br />
of choice<br />
‡ Multi-Mastercycler-Networking with a PC<br />
‡ Intuitive Windows ® -like interface<br />
‡ Icon-based creation and edit of programs<br />
‡ Simultaneous control of all or selected<br />
Mastercycler pro cyclers<br />
‡ GLP-conformity documentation of all PCR experiments<br />
‡ User password protection<br />
‡ Real-time acquisition of temperature data<br />
‡ Easy installation and uninstallation<br />
‡ Compatible with Windows<br />
‡ Easy transfer of PCR programs from the Control Panel<br />
to the PC<br />
Description<br />
Complete Mastercycler pro package (includes cycler and control panel)<br />
Catalog No.<br />
Mastercycler pro and Control Panel, 120 V, 50–60 Hz 950040015<br />
Mastercycler pro S and Control Panel, 120 V, 50–60 Hz 950040025<br />
Mastercycler pro 384 and Control Panel, 120 V, 50–60 Hz<br />
Individual Mastercycler pro modules<br />
950040035<br />
Control Panel, includes connection cable 950030050<br />
Mastercycler pro*, 120 V, 50–60 Hz 950030010<br />
Mastercycler pro S*, 120 V, 50–60 Hz 950030020<br />
Mastercycler pro 384*, 120 V, 50–60 Hz<br />
Accessories<br />
950030030<br />
CAN_Bus connection cable, 50 cm 950014008<br />
CAN_Bus connection cable, 150 cm 950014016<br />
Self Test Dongle 950030040<br />
MultiMediaCard, 64 MB 960002008<br />
CycleManager pro, incl. installation manual, online help, and connection cable<br />
Service<br />
Extended Warranty, see page 215 for details<br />
Preventive Maintenance, see page 215 for details<br />
950017007<br />
* A Control Panel or CycleManager pro software (both sold separately) is required for operation; CAN_Bus connection cables are required to link cyclers together as a network.<br />
This product is licensed under U.S. patent Nos. 5,525,300, 5,779,981 and 6,054,263. The heated cover device is licensed under US 5,552,580 and foreign equivalents. The user of the <strong>Eppendorf</strong> Mastercycler pro<br />
might require additional rights for kits, reagents and other components required for his/her application. Such accompanying rights for these kits, reagents and other may be obtained by the respective holder of such<br />
rights. No rights are conveyed expressly, by implication or estoppel to any patents on real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent or kit. Mastercycler pro<br />
upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts.<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 Thermocyclers
Thermocyclers<br />
4<br />
192 Sample Handling<br />
Mastercycler® nexus<br />
NEW!<br />
‡ Stand-by function saves energy<br />
‡ Graphical user interface<br />
‡ USB port for data transfer<br />
or mouse connection<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
‡ E-mail notification
Mastercycler® nexus<br />
‡ One hand operation<br />
‡ flexlid adjusts to<br />
height of consumables<br />
‡ Connect up to 2 Mastercycler nexus eco<br />
units to increase throughput<br />
Sample Handling 193<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 Thermocyclers
Thermocyclers<br />
4<br />
194 Sample Handling<br />
Mastercycler® nexus<br />
Description<br />
The Mastercycler nexus offers the reliability and ease-of-use<br />
of an <strong>Eppendorf</strong> cycler with low energy consumption, easy<br />
expandability to 3 units and a small footprint make it not only fit<br />
to your budget, but also to your precious lab space. It can handle<br />
all kinds of PCR consumables, from low volume all the way up<br />
to 0.5 mL PCR tubes. All common PCR plates fit—and if your<br />
throughput becomes even higher, you can just connect 1 or<br />
2 Mastercycler nexus eco to your Mastercycler nexus.<br />
Product features<br />
‡ Flexlid concept allows use of different consumables<br />
with automatic height adjustment of the heated lid<br />
‡ Intuitive graphic programming<br />
‡ Small footprint<br />
‡ Universal block for ultimate flexibility<br />
‡ Connect up to 2 Mastercycler nexus eco units to your<br />
existing unit<br />
‡ Available with 12 column gradient<br />
‡ E-mail notification<br />
‡ Average PCR run power consumption of 0.154 kW/h<br />
Further information under<br />
www.eppendorf.ca/mastercyclernexus<br />
For additional information see “Application tips for PCR”<br />
on page 393.<br />
Ordering information<br />
Description Catalog No.<br />
Mastercycler nexus gradient, 120 V, 50/60 Hz 6331000025<br />
Mastercycler nexus, 120 V, 50/60 Hz 6333000022<br />
Mastercycler nexus eco 1 , 120 V, 50/60 Hz 6332000029<br />
Mastercycler nexus flat block, 120 V, 50/60 Hz 6335000020<br />
Self test dongle 950030040<br />
CAN-Bus connection cable 1 , 50 cm 950014008<br />
CAN-Bus connection cable 1 , 150 cm 950014016<br />
1 Requires Mastercycler nexus gradient or Mastercycler nexus to operate.<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
Gradient with SteadySlope ® Technology<br />
The gradient option of the Mastercycler nexus features the<br />
SteadySlope technology to ensure that the heating and<br />
cooling ramp rates are identical in both gradient and normal<br />
operation. Thus, you can expect identical temperature control<br />
characteristics in both optimization and routine experiments.<br />
Programming the gradient is easy and intuitive – so that novice<br />
users can get started quickly and safely.<br />
Easy testing<br />
The self test function of the Mastercycler nexus provides the<br />
ability to check the peltier elements of the thermal block easily.<br />
This feature tests the functionality of all six peltier elements and<br />
provides a corresponding certificate. This way, the user can<br />
document the homogeneity of the instrument between regular<br />
testing intervals. In addition, <strong>Eppendorf</strong> ® offers a Temperature<br />
Validation System as an external testing device.<br />
Certified Quality<br />
‡ Individual, documented quality control certificate<br />
‡ Calibration according to national and international standards:<br />
NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK)<br />
‡ UL/CUL listed<br />
High quality consumables<br />
Consumables of high quality, e.g. <strong>Eppendorf</strong> twin.tec ® PCR<br />
plates, help to improve PCR results. For example, the raised rims<br />
of the <strong>Eppendorf</strong> twin.tec PCR plates are optimized to guarantee<br />
tight sealing in all wells. For further information, please visit<br />
www.eppendorf.ca/pcr
Mastercycler® ep realplex<br />
Product features<br />
High-speed real-time PCR<br />
‡ Load your plate and receive analyzed results in less than<br />
half an hour!<br />
‡ Fast and easy programming<br />
‡ Fast optical detection capabilities eliminate the need<br />
for lengthy extension/elongation<br />
‡ Aluminum block model offers faster ramp rates than<br />
nearly any other supplier<br />
‡ “S” model for highest speed<br />
‡ Complete more experiments per day<br />
No restrictions<br />
‡ Use the reagents, kits and consumables of your choice<br />
‡ Even the high-speed “S” model does not lock you in to<br />
special kits or plates<br />
Gradient<br />
‡ Optimize your protocols in a single plate/experiment<br />
‡ Test more distinct temperatures at once with Triple<br />
Circuit thermal block design<br />
Application<br />
* New! Modified slip cover (light-transmissive cover for the optical openings of the heated plates assembly, US patent pending).<br />
Licensed and authorized for real-time PCR; see next page.<br />
‡ Quantitative real-time PCR<br />
96-well<br />
silver block<br />
Sample Handling 195<br />
96-well<br />
aluminum block<br />
Description<br />
Continuing the <strong>Eppendorf</strong> ® focus on best design, the<br />
Mastercycler ep realplex combines the highest performance<br />
with the most advanced features and capabilities of a 96-well<br />
real-time PCR system—and in a compact class!<br />
Highly sensitive optics<br />
‡ The new optical system of the Mastercycler ep realplex<br />
offers a new, exclusive Particle Protection* and<br />
Photomultipliers of the latest generation<br />
Highest reproducibility<br />
‡ LEDs keep consistent intensity over time—other light<br />
sources lose intensity over time<br />
‡ Each LED is normalized to equal intensity<br />
‡ Over 55 years of <strong>Eppendorf</strong> expertise in optical systems<br />
Intuitive software<br />
‡ All analysis modules included (see pages 197 and 198)<br />
‡ Highly accurate calculation of Ct-values using<br />
the CalQplex ® algorithm<br />
‡ “QUICK START” capability<br />
<strong>Eppendorf</strong> design<br />
‡ Quiet operation, 554 dB(A)<br />
‡ Small footprint<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 Thermocyclers
Thermocyclers<br />
4<br />
196 Sample Handling<br />
Mastercycler® ep realplex<br />
Complete real-time PCR system twin.tec ® real-time PCR plate<br />
96 individual LED array realplex filter wheels<br />
Mastercycler ep realplex 2 and realplex 2 S<br />
‡ These two-channel systems meet the needs of most<br />
laboratories (emission 520/550 nm)<br />
‡ Designed for use with SYBR ® Green assays,<br />
FAM-labeled probes or single and duplex<br />
probe reactions<br />
‡ High reproducibility is possible at 10 μL total<br />
reaction volumes with cali brated <strong>Eppendorf</strong> ® pipettes;<br />
for lower volumes please inquire about automated<br />
real-time PCR set-up<br />
‡ 2-year warranty comes standard—best in its class!<br />
‡ Lifetime warranty on LED array<br />
Mastercycler ep realplex 4 and realplex 4 S<br />
‡ This four-channel system (emission 520/550/580/605 nm)<br />
provides full flexibility for multiplexing<br />
‡ High reproducibility is possible at 10 μL total<br />
reaction volumes with cali brated <strong>Eppendorf</strong> pipettes;<br />
for lower volumes please inquire about automated<br />
real-time PCR set-up<br />
‡ 2-year warranty comes standard—best in its class!<br />
‡ Lifetime warranty on LED<br />
Practice of the patented polymerase chain reaction (PCR) process requires a license. The <strong>Eppendorf</strong> [or appropriate trademark] Thermal Cycler is an Authorized<br />
Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in<br />
accordance with the label rights accompanying such reagents. This is a Licensed Real-Time Thermal Cycler under Applera’s United States Patent No. 6,814,934 and<br />
corresponding claims in non-U.S. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed<br />
expressly, by implication or by estoppel under any other patent claim.<br />
The purchase of this product includes a limited, non-transferable Iicense under U.S. Patent No. 5,871,908 and all continuations and divisionals, and corresponding<br />
claims in patents and patent applications outside the United States, owned by Roche Diagnostics GmbH, for internal research use or for non-in vitro diagnostics<br />
applications with authorized reagents with regard to Melting Curve Analysis. No right is conveyed, expressly, by implication or estoppel, under any other patent or<br />
patent claims owned by Roche Diagnostics GmbH, or by any other Party.<br />
Order direct from <strong>Eppendorf</strong> <strong>Canada</strong> Ltd.<br />
Product appearance, specifications, and/or prices are subject to change without notice.
Mastercycler® ep realplex<br />
<strong>Eppendorf</strong>’s Mastercycler ep realplex software is intuitive and<br />
user-friendly. The Windows ® -like interface allows for a clear<br />
and comprehensive organization of multiple viewing windows.<br />
The Plate layout screen is designed like a 96-well PCR plate<br />
making labeling and identification easy. This screen also<br />
provides a helpful “Hint” box in the bottom corner making<br />
set up simple for users of any level. Once your experiment<br />
has begun the Monitoring screen allows you to visualize the<br />
results in real-time as they appear, and you can interrupt the<br />
run once the desired results have been obtained!<br />
‡ Plate layout<br />
The clear and comprehensive organization of<br />
multiple viewing windows, as well as intuitive<br />
options for selection within each view, enable<br />
easy and fast plate setup when creating assays.<br />
‡ Quantification/relative quantification<br />
This analysis module performs calculations<br />
from raw data for a variety of assays, including<br />
absolute quantification of DNA or relative<br />
quantification of gene expression based<br />
on the ADCt method.<br />
Sample Handling 197<br />
The Mastercycler ep realplex software is designed to be<br />
intuitive for real-time PCR users of all levels, making data<br />
collection and analysis easy, efficient and comprehensive.<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 | Instruments | PCR<br />
‡ Raw data<br />
This software module displays the generated<br />
raw data in real-time, which means that you<br />
can immediately evaluate your real-time PCR<br />
and interrupt the reaction as soon as the<br />
desired result is obtained.<br />
‡ Gene identification<br />
Selecting this analysis module option generates<br />
allele-discrimination results. Data previously<br />
generated in melting curve analyses, endpoint<br />
measurements or Ct-value determinations<br />
serves as the basis.<br />
4 Thermocyclers
Thermocyclers<br />
4<br />
198 Sample Handling<br />
Mastercycler® ep realplex<br />
‡ Endpoint<br />
The analysis module option for endpoint<br />
measurements instructs Mastercycler ep<br />
realplex to serve as a “plate fluorometer”<br />
and aids in the determination of absolute<br />
fluorescence intensities, even with samples<br />
previously amplified on standard thermocyclers.<br />
‡ +/– Assay<br />
Define critical threshold values through this<br />
analysis module option to differentiate between<br />
positive and negative samples (e.g., for the<br />
detection of pathogens). Data from previous<br />
endpoint measurements serves as the source<br />
for such determinations.<br />
The Mastercycler ep realplex software includes various analysis modules making this system versatile and ideal<br />
for multiple applications. Protein characterization can now be added to this list; with the use of our “Inverted Data”<br />
tool all protein reactions can be easily evaluated with our system (see images below).<br />
‡ Protein Characterization<br />
Inverted data flips the negative peaks so the threshold is no longer below zero and the data may be properly evaluated.<br />
Licensed for real-time PCR, see page 196.<br />
Order direct from <strong>Eppendorf</strong> <strong>Canada</strong> Ltd.<br />
Product appearance, specifications, and/or prices are subject to change without notice.
Mastercycler® ep realplex<br />
Technical specifications<br />
Optical module<br />
Excitation source 96 LEDs (470 nm)<br />
Emission filters 520/550 nm (realplex2 )<br />
520/550/580/605 nm (realplex4 )<br />
Detector 1 photomultiplier tube (realplex2 )<br />
2 photomultiplier tubes (realplex4 )<br />
Dynamic range 8 orders of magnitude from starting copy number<br />
Sensitivity<br />
Thermomodule<br />
≤50 fmol fluorescein<br />
Sample capacity 96 x 0.2 mL PCR tubes or one 96-well PCR plate<br />
(unskirted, semi-skirted, skirted—as per SBS format)<br />
Temperature control range of block 4–99 °C<br />
Degree range of gradient, maximum 1–20 °C (aluminum block)<br />
1–24 °C (silver block)<br />
Temperature control range of gradient 30–99 °C<br />
Temperature of lid 105 °C<br />
Block homogeneity 20–72 °C ±0.3 °C,<br />
90 °C ±0.4 °C<br />
Control accuracy ±0.2 °C<br />
Heating speed* approx. 4 °C/s (aluminum block)<br />
approx. 6 °C/s (silver block)<br />
Cooling speed*<br />
Complete system<br />
approx. 3 °C/s (aluminum block)<br />
approx. 4.5 °C/s (silver block)<br />
Dimensions (W x D x H) 10.24 in x 16.14 in x 15.59 in/26 cm x 41 cm x 39.6 cm<br />
Total weight 52.91 lb/24 kg<br />
Voltage requirements 115 V, 50 Hz–60 Hz<br />
Power consumption 800 W<br />
* Measured at block<br />
Ordering information<br />
Description Catalog No.<br />
Mastercycler ep realplex<br />
Mastercycler ep realplex2 , with aluminum block and two emission filters Please Inquire<br />
Mastercycler ep realplex2 S, with silver block and two emission filters Please Inquire<br />
Mastercycler ep realplex4 , with aluminum block and four emission filters Please Inquire<br />
Mastercycler ep realplex4 S, with silver block and four emission filters Please Inquire<br />
System accessories<br />
Real-time PCR consumables, see page 206 or visit www.eppendorf.ca<br />
Service<br />
Performance plans, see page 214 for details<br />
Please contact <strong>Eppendorf</strong> <strong>Canada</strong> at 800-263-8715 to receive a quotation for the Mastercycler ep realplex system.<br />
Sample Handling 199<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 Thermocyclers
4 PCR Plates<br />
200 Sample Handling<br />
twin.tec® PCR Plates<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
4 | Consumables | PCR<br />
General product features<br />
‡ One-piece design: combining a polycarbonate frame<br />
and polypropylene wells for optimum performance<br />
‡ Extremely thin-walled polypropylene wells guarantee<br />
optimum heat transfer to the sample<br />
‡ Exceptionally solid and torque-resistant<br />
polycarbonate frame<br />
‡ Improved well-to-well tolerance<br />
‡ Minimal binding of DNA, RNA and enzymes<br />
‡ Cut-away corner and alphanumeric grid-referencing<br />
‡ Raised well rims for effective sealing, also reduces<br />
risk of cross-contamination<br />
‡ Autoclavable (121 °C, 20 min)<br />
‡ Certified free of any detectable human DNA, DNase,<br />
RNase and PCR inhibitors*<br />
‡ Available with barcode (upon request)<br />
‡ Ideal for quantitative real-time PCR as well<br />
* Certificate, test procedures and detailed information available on request.<br />
Additional features of twin.tec PCR Plate 96,<br />
skirted and semi-skirted<br />
‡ Compatible with automated systems<br />
‡ Skirted for label or bar code<br />
‡ Fits most thermal cyclers and the MegaBACE ®<br />
capillary sequencer<br />
‡ Within SBS footprint recommendations<br />
(127.76 x 85.47 mm ±0.25 mm)<br />
‡ Stackable<br />
‡ Eight holes in the skirt aid plate positioning and removal<br />
from the thermal cycler block<br />
‡ Low profile design enhances efficiency of PCR and<br />
enables the highest efficiency for small sample volumes<br />
‡ 150 μL max. well volume (when used with cap strips, skirted)<br />
‡ 250 μL max. well volume (when used with cap strips,<br />
semi-skirted)<br />
Additional features of twin.tec PCR Plate 384<br />
‡ Continuous skirt for compatibility with<br />
automated systems<br />
‡ Skirted for label or bar code<br />
‡ Stackable<br />
‡ Within SBS footprint recommendations<br />
‡ Ideal for most common 384-well thermal cyclers,<br />
particularly for <strong>Eppendorf</strong> Mastercycler ® pro 384<br />
(see page 189)<br />
‡ 45 μL max. well volume<br />
For more information visit www.eppendorf.ca/twintecplates
twin.tec® PCR Plates<br />
Sample Handling 201<br />
Product features of twin.tec PCR Plate 96, unskirted<br />
‡ Fits on a wide variety of thermal cyclers<br />
‡ OptiTrack ® matrix: high contrast labeling of alphanumeric<br />
grid for easier sample tracking<br />
‡ Available with regular (250 μL) or low profile (150 μL) wells<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 | Consumables | PCR<br />
Product features of twin.tec PCR Plate 96, unskirted,<br />
divisible<br />
‡ Can be divided in 4 segments of 24 wells each<br />
‡ Fits on a variety of thermal cyclers<br />
‡ OptiTrack matrix: high contrast labeling of alphanumeric<br />
grid for easier sample tracking<br />
‡ Available with regular (250 μL) or low profile (150 μL) wells<br />
Product features of twin.tec microbiology PCR Plate<br />
‡ Sterile, individually blistered plates<br />
‡ Additionally free of bacterial DNA<br />
‡ For all applications that need ultimate purity or certified<br />
absence of bacterial DNA<br />
4 PCR Plates
4 PCR Plates<br />
202 Sample Handling<br />
twin.tec® PCR Plates<br />
Technical specifications. All dimensions in millimeters.<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.
twin.tec® PCR Plates<br />
Ordering information<br />
Description Catalog No.<br />
twin.tec PCR Plate 96*, skirted (clear wells), 25 pcs., (150 μL max. volume)<br />
Clear frame 951020401<br />
Yellow frame 951020427<br />
Green frame 951020443<br />
Blue frame 951020460<br />
Red frame<br />
twin.tec PCR Plate 96*, skirted (black wells), 25 pcs., (150 μL max. volume)<br />
951020486<br />
Yellow frame<br />
twin.tec PCR Plate 96*, semi-skirted (colorless wells), 25 pcs., (250 μL max. volume)<br />
951020508<br />
Clear frame 951020303<br />
Yellow frame 951020320<br />
Green frame 951020346<br />
Blue frame 951020362<br />
Red frame<br />
twin.tec PCR Plate 384* (colorless wells), 25 pcs., (45 μL max. volume)<br />
951020389<br />
Clear frame 951020702<br />
Yellow frame 951020711<br />
Green frame 951020729<br />
Blue frame 951020737<br />
Red frame<br />
twin.tec PCR Plate 96, unskirted low profile, 20 pcs., (150 μL max. volume)<br />
951020745<br />
Clear frame 0030133307<br />
Yellow frame 0030133315<br />
Green frame 0030133323<br />
Blue frame 0030133331<br />
Red frame 0030133340<br />
Clear frame, divisible 0030133358<br />
Blue frame, divisible<br />
twin.tec PCR Plate 96, unskirted, 250 µL, 20 pcs., (250 μL max. volume)<br />
0030133382<br />
Clear frame 0030133366<br />
Blue frame 0030133390<br />
Clear frame, divisible 0030133374<br />
Blue frame, divisible<br />
twin.tec microbiology PCR Plate 96, skirted, 10 pcs., (150 μL max. volume)<br />
0030133404<br />
Clear frame 0030129300<br />
Blue frame<br />
twin.tec microbiology PCR Plate 96, semi-skirted, 10 pcs., (250 μL max. volume)<br />
0030129318<br />
Clear frame 0030129326<br />
Blue frame<br />
twin.tec microbiology PCR Plate 384, 10 pcs., (45 μL max. volume)<br />
0030129334<br />
Clear frame 0030129342<br />
Blue frame 0030129350<br />
* <strong>Eppendorf</strong> owns protective rights under European Patent EP 1 161 994 and US patents US 7,347,977 and US 6,340,589<br />
For more information on <strong>Eppendorf</strong> purity levels, see pages 394 to 397, or visit www.eppendorf.ca/purity.<br />
Sample Handling 203<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 PCR Plates
4 PCR Tubes<br />
204 Sample Handling<br />
PCR Tubes<br />
More space for labeling due to improved lid design<br />
Fig. 1: 0.5 mL PCR Tube<br />
writing surface<br />
Product features of 0.5 mL PCR Tubes, thin-walled Product features of 0.2 mL PCR Tubes, thin-walled<br />
‡ Special space-saving lid design<br />
‡ For maximum capacity in the universal blocks of<br />
<strong>Eppendorf</strong> Mastercyclers ® and other 0.5 mL blocks<br />
‡ High transparency<br />
‡ Etched lid for labeling<br />
‡ Tight sealing<br />
‡ Easy to open<br />
‡ For use with all thermal cyclers with 0.5 mL block format<br />
‡ Certified free from human DNA, DNase, RNase and<br />
PCR inhibitors*<br />
Description<br />
Original <strong>Eppendorf</strong> ® 0.2 mL and 0.5 mL PCR Tubes are<br />
manufactured to the highest quality standards. These thin-walled<br />
polypropylene tubes ensure efficient and homogeneous heat<br />
transfer of the sample, thanks to their even wall thickness and<br />
smooth wall surface. Heat-sealed bags of tubes are enclosed in<br />
boxes that are also sealed with a PCR clean sticker to ensure the<br />
highest degree of purity.<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
Contamination shield prevents contact with inner lid surface<br />
Fig. 2: 0.2 mL PCR Tube<br />
writing surface<br />
‡ Contamination shield on hinged lid<br />
‡ Defined lid position due to special hinge<br />
‡ High transparency even on the tube base<br />
‡ Etched lid for labeling<br />
‡ Tight sealing<br />
‡ Easy to open<br />
‡ For use with all thermal cyclers with 0.2 mL block format<br />
‡ Also available in 8-tube strip format<br />
‡ Certified free from human DNA, DNase, RNase and<br />
PCR inhibitors*<br />
* Certificate, test procedures and detailed information available on request.<br />
defined lid position due<br />
to special hinge
PCR Tubes<br />
Ordering information<br />
Product features of 0.1 mL PCR Tube Strips<br />
‡ 8 reaction tubes in strip-format —ideal for small<br />
sample volumes<br />
‡ Easily adaptable for automation<br />
‡ Sealable using flat or domed strip caps<br />
‡ Certified free from human DNA, DNase, RNase<br />
and PCR inhibitors*<br />
* Certificate, test procedures and detailed information available on request.<br />
Description<br />
0.1 mL <strong>Eppendorf</strong><br />
Catalog No.<br />
® PCR Tubes<br />
PCR Tube Strips 0.1 mL, without lids (10 × 12 strips) 0030124804<br />
PCR Tube Strips 0.1 mL plus Cap Strips, domed (10 × 12 strips each) 0030124812<br />
PCR Tube Strips 0.1 mL plus Cap Strips, flat (10 × 12 strips each)<br />
0.2 mL <strong>Eppendorf</strong> PCR Tubes<br />
0030124820<br />
Clear, 1,000 pcs. 951010006<br />
8-tube strip, for 0.2 mL PCR Tubes, pack of 120 (960 tubes total), with dome hinged cap<br />
0.5 mL <strong>Eppendorf</strong> PCR Tubes, thin-walled with hinged lid<br />
951010022<br />
Clear, 500 pcs. 0030124537<br />
Sample Handling 205<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 PCR Tubes
4 PCR Plates<br />
206 Sample Handling<br />
twin.tec® real-time PCR Plates<br />
Product features<br />
‡ White wells for better reflection<br />
‡ High mechanical stability makes them torque-resistant<br />
‡ Raised rims for effective sealing<br />
‡ “Skirted” (stackable) and “semi-skirted” plates<br />
‡ Optimal heat transfer due to reduced wall thickness<br />
‡ Autoclavable (121 °C, 20 min)<br />
‡ Certified free of any detectable human DNA, DNase,<br />
RNase and PCR inhibitors*<br />
‡ Available with barcode (upon request)<br />
* Certificate, test procedures and detailed information available on request.<br />
Reflection is up to 10-fold higher than with frosted wells.<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
Description<br />
<strong>Eppendorf</strong> ® twin.tec real-time PCR Plates 1 offer all the advantages<br />
of regular twin.tec plates and give you the advantage of white<br />
wells for your real-time PCR. The limiting factor in low volume<br />
real-time PCR is often the remaining intensity of fluorescence.<br />
The white wells of the <strong>Eppendorf</strong> twin.tec real-time PCR plates<br />
reflect fluorescence much better than clear or frosted wells.<br />
Thus, lower levels of fluorescence are still detectable with<br />
the same instrument—just by using the right consumables.<br />
Additionally, white wells significantly reduce interfering<br />
background fluorescence and lead to increased homogeneity<br />
of replicates and reproducible results. The rigidity of the<br />
polycarbonate frame ensure easy and reliable handling—<br />
manually or automated. The polypropylene wells guarantee<br />
excellent and fast temperature transfer to the sample.<br />
1 <strong>Eppendorf</strong> owns protective rights under European Patent EP 1 161 994 and US patents US 7,347,977<br />
and US 6,340,589<br />
For more information visit www.eppendorf.ca/realtime
twin.tec® real-time PCR Plates<br />
Ordering information<br />
Description<br />
twin.tec 96 real-time PCR Plates, skirted, set of 25 pcs.<br />
Catalog No.<br />
White frame 951022015<br />
Blue frame 951022003<br />
Black frame<br />
twin.tec 96 real-time PCR Plates, semi-skirted, set of 25 pcs.<br />
951022027<br />
White frame 951022055<br />
Blue frame 951022043<br />
Black frame<br />
twin.tec 96 real-time PCR Plates, unskirted low profile, set of 20 pcs. (150 μL max. volume)<br />
951022067<br />
White frame 0030132700<br />
Blue frame 0030132718<br />
Black frame<br />
twin.tec 384 real-time PCR Plates, skirted, set of 25 pcs.<br />
0030132726<br />
White frame 0030132734<br />
Blue frame 0030132742<br />
Black frame 0030132750<br />
For more information on <strong>Eppendorf</strong> purity levels, see pages<br />
394 to 397, or visit www.eppendorf.ca/purity.<br />
Masterclear® Cap Strips and real-time PCR Tube Strips<br />
Ordering information<br />
Product features<br />
Masterclear Cap Strips<br />
Sample Handling 207<br />
‡ Inverted dome to avoid scratching of the optical surface<br />
‡ Inverted dome to reduce dead volume of the tubes<br />
‡ Optimized for ultimate light transmission<br />
‡ Optically clear for real-time PCR<br />
real-time PCR Tube Strips<br />
‡ White wells for better reflection—ideal for use with lower<br />
sample volume<br />
‡ High mechanical stability<br />
‡ Extremely thin walls for optimal heat transfer<br />
‡ 0.1 mL Tube Strips<br />
Description Catalog No.<br />
Masterclear Cap Strips, 10 x 12 pieces 951022089<br />
real-time PCR Tube Strips, without caps, 10 x 12 pieces 951022102<br />
Masterclear Cap Strip and real-time PCR Tube Strip, 10 x 12 pieces each 951022109<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 PCR Plates
4 Heat Sealing Materials<br />
208 Sample Handling<br />
Heat Sealer<br />
Heat sealing materials<br />
Product features<br />
‡ Hermetic heat sealing of multiwell plates<br />
‡ Eliminates evaporation in PCR, reducing<br />
cross-contamination<br />
‡ Ideal for sample transportation and storage<br />
‡ Seals 96- and 384-well plates<br />
‡ Suitable for plates of different heights<br />
‡ Optimum sealing with <strong>Eppendorf</strong> ® Heat Sealing Foils<br />
and Films at a preset temperature<br />
‡ Compact design, portable device<br />
‡ Integrated thermostat prevents overheating<br />
‡ Heating plate faces away for safety<br />
‡ Improved mechanics for even easier sealing<br />
Product features<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
Ordering information<br />
Description Catalog No.<br />
Heat Sealer, 115 V/50 Hz 951023078<br />
Base plate, for 384-well plate 951023086<br />
‡ Hermetic sealing of multiwell plates, especially<br />
recommended for low reaction volumes<br />
‡ Best protection against evaporation during PCR<br />
‡ Certified free of human DNA, DNase, RNase and<br />
PCR inhibitors*<br />
‡ PCR foil is both pierceable and peelable<br />
‡ Maximum adhesiveness at high temperature during PCR<br />
* Certificate, test procedures and detailed information available upon request.<br />
Ordering information<br />
Description Catalog No.<br />
Heat Sealing PCR Film, 100 pcs. 0030127838<br />
Heat Sealing PCR Foil, 100 pcs. 0030127854
Heat sealing materials<br />
Technical specifications<br />
Description Heat Sealing PCR Film Heat Sealing PCR Foil<br />
Packaging unit 1 x 100 pcs. 1 x 100 pcs.<br />
Features<br />
‡ Optically clear polyester/<br />
polypropylene laminate<br />
‡ Extremely stable sealing option —<br />
cannot be removed or pierced<br />
‡ Laminated aluminium foil<br />
‡ Easily removable<br />
‡ Easily pierceable, even with<br />
multichannel pipettes<br />
Sample Handling 209<br />
Seal integrity –80 °C to 100 °C –80 °C to 100 °C<br />
Sealing time with<br />
<strong>Eppendorf</strong> ® Heat Sealer<br />
4 sec. 4 sec.<br />
Weldable materials<br />
‡ Polypropylene<br />
‡ Polypropylene<br />
Special applications<br />
‡ Colorimetric applications<br />
‡ Recommended for use in automated<br />
‡ Fluorescence applications,<br />
including real-time PCR<br />
‡ Storage of hazardous samples<br />
work processes<br />
PCR Film (self-adhesive) and PCR Foil (self-adhesive)<br />
Product features<br />
‡ Effective adhesive sealing prevents evaporation loss<br />
during reaction and storage<br />
‡ Removal from the plate with no residue<br />
‡ Certified free of human DNA, DNase, RNase and<br />
PCR inhibitors*<br />
PCR Film<br />
‡ Sample can be visually monitored through the<br />
transparent film<br />
PCR Foil<br />
‡ Easily pierced<br />
‡ No sticking of the pipette tip (ideal for automated systems)<br />
* Certificate, test procedures and detailed information available upon request.<br />
Ordering information<br />
Description Catalog No.<br />
PCR Film (self-adhesive), 0030127811<br />
100 pcs.<br />
PCR Foil (self-adhesive), 0030127820<br />
100 pcs.<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 Heat Sealing Materials
4 Heat Sealing Materials<br />
210 Sample Handling<br />
Masterclear® real-time PCR Film<br />
Cap Strips<br />
Capping Aid<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
4 | Consumables | PCR<br />
Product features<br />
‡ Optimized for maximum light transmission<br />
‡ Effectively forms a tight seal to reduce evaporation<br />
‡ Maximum handling comfort due to optimized packaging<br />
‡ Optically clear for real-time PCR<br />
Ordering information<br />
Description Catalog No.<br />
Masterclear real-time PCR Film, 951022115<br />
(self-adhesive), 100 pieces<br />
Product features<br />
‡ Strips with eight microcaps for 0.1 and 0.2 mL wells<br />
‡ Easy and rapid sealing of <strong>Eppendorf</strong> ® twin.tec ® PCR<br />
Plates, PCR strips and other standard multiwell plates<br />
‡ Flat Cap Strips are suitable for real-time PCR<br />
‡ Autoclavable (121 °C, 20 min)<br />
Ordering information<br />
Description Catalog No.<br />
Cap Strips (8-strips),<br />
0030124839<br />
domed, 10 x 12 pcs.<br />
Cap Strips (8-strips),<br />
0030124847<br />
flat, 10 x 12 pcs.<br />
Product features<br />
‡ Tool for easy and fast sealing of 0.2 mL PCR test tubes<br />
and twin.tec PCR Plates with Cap Strips<br />
‡ Several wells and tubes can be sealed simultaneously<br />
‡ Minimal force required for an effective seal<br />
‡ Minimizes losses due to evaporation<br />
‡ Certified free of human DNA, DNase, RNase and<br />
PCR inhibitors*<br />
Ordering information<br />
Description Catalog No.<br />
Capping Aid, 1 pc. 951023108<br />
* Certificate, test procedures and detailed information available upon request.
Work tray and frame<br />
PCR Rack<br />
Product features<br />
Sample Handling 211<br />
‡ Frame with work tray serves as a 2-part polycarbonate<br />
rack for 96 x 0.2 mL PCR tubes<br />
‡ All tubes can be directly transferred to a thermoblock<br />
‡ Autoclavable (121 °C, 20 min)<br />
Ordering information<br />
Product features<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 | Consumables | PCR<br />
Description Catalog No.<br />
Work tray, for 96 x 0.2 mL<br />
PCR tubes, set of 10<br />
951010031<br />
Frame for work tray, set of 5 951010049<br />
‡ Rack for handling and storing tubes and plates<br />
‡ For 0.2 mL, 0.3 mL and 0.5 mL tubes, as well as<br />
96-well plates<br />
‡ Stackable<br />
‡ Can be stored down to –90 °C<br />
‡ Autoclavable (121 °C, 20 min)<br />
Ordering information<br />
Description Catalog No.<br />
PCR Rack, 10 pcs. 0030124545<br />
4 Heat Sealing Materials
4 Adapters<br />
212 Sample Handling<br />
in situ Adapter<br />
Technical specifications<br />
Sample capacity Max. 4 glass slides 76 x 26 mm<br />
Temperature range 4–99 °C<br />
Temperature distribution 20–72 °C ±0.6 °C<br />
across the adapter<br />
95 °C<br />
±1.0 °C<br />
Temperature control speed ≥1.0 °C/s (heating)<br />
measured on the adapter ≥0.7 °C/s (cooling)<br />
Temperature Validation System for Mastercycler Family<br />
Ordering information<br />
Order from your laboratory supply dealer.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
4 | Consumables | PCR<br />
Description Catalog No.<br />
Temperature Validation System<br />
(96-well probe included)<br />
950008059<br />
Temperature sensor, 384-well 950008041<br />
Product features<br />
‡ Easy to handle<br />
‡ Perfect fit for optimal heat transfer<br />
‡ Software supported, easy to program, extremely flexible<br />
‡ Can accommodate 4 standard glass slides<br />
‡ Transparent covering hood—in situ frames trap moisture<br />
(sold separately)<br />
Description<br />
The in situ Adapter can be used to convert your Mastercycler ®<br />
or Mastercycler nexus to an in situ device as easily as if you<br />
were to insert a PCR plate. And the quality of the heat transfer<br />
is equally as high! With precise block homogeneity, the in situ<br />
Adapter has room for up to four standard glass slides.<br />
Ordering information<br />
Description Catalog No.<br />
in situ Adapter<br />
950007052<br />
for Mastercycler nexus<br />
and Mastercycler<br />
in situ Frames Please Inquire<br />
Product features<br />
‡ Single-well probe<br />
‡ Sensor for 0.2 mL tube position<br />
‡ Optional: sensor for 384-well block, sold separately<br />
‡ Simple, rapid validation and calibration<br />
‡ Autocalibration with Mastercycler software, version<br />
2.10 or higher<br />
‡ Can be third-party calibrated<br />
Description<br />
The Temperature Validation System for the entire Mastercycler,<br />
Mastercycler nexus, Mastercycler ep and Mastercycler pro<br />
families enables simple and rapid validation and calibration of<br />
block temperature. The program for automatic measurement<br />
and calibration is integrated into the thermal cycler software,<br />
thus providing an accurate and precise system that meets all<br />
documentation and detection requirements in the laboratory.
Temperature Verification System<br />
Product features<br />
‡ Verification of the temperature control of the thermoblock<br />
and the heated lid in accordance with the appropriate<br />
<strong>Eppendorf</strong> ® specifications<br />
‡ Generation of certificates of conformity and of measuring<br />
reports for the thermocyclers<br />
‡ The temperature control of the thermoblock can be<br />
adjusted automatically, if necessary<br />
‡ Recording of a temperature profile of user defined<br />
programs in 14 thermoblock positions<br />
‡ Increased number of measuring points: 14 block<br />
thermosensors and 1 lid thermosensor<br />
‡ Temperatures of all positions are measured<br />
simultaneously—short data acquisition time per cycler<br />
Ordering information<br />
Description<br />
Temperature Verification System<br />
Catalog No.<br />
includes 96-well sensor plate 6328000006<br />
includes 384-well sensor plate 6328000014<br />
Sensor plate for Temperature Verification System<br />
96-well 6328071000<br />
384-well 6328072007<br />
Sample Handling 213<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 | Consumables | PCR<br />
Description<br />
The <strong>Eppendorf</strong> Temperature Verification System can<br />
perform a verification, and if necessary an adjustment,<br />
of the Mastercycler ® pro, Mastercycler ep, Mastercycler nexus<br />
and Mastercycler ep realplex instruments according to the<br />
manufacturer’s specifications. This system can also record<br />
temperature profiles and measure ramp rates to ensure that<br />
the thermal cycler is always at its peak performance level.<br />
The <strong>Eppendorf</strong> Temperature Verification System has 14<br />
block thermosensors and 1 lid thermosensor, all positions<br />
are measured simultaneously for rapid data acquisition.<br />
4 Thermocycler Software
Services – PCR<br />
44<br />
214 Sample Service products Handling<br />
Mastercycler® ep realplex Performance Plans and Services<br />
Value of Service<br />
From warranty plus Protection that provides a LED light source<br />
for life and a 2-year system warranty, to service plans for<br />
every lab need, our goal is to help you achieve reproducible<br />
results over an extended period of time. Reliable quantification<br />
is critical in qPCR and <strong>Eppendorf</strong> ® Service will keep your<br />
instrument in peak performance.<br />
Choose a plan that’s right for you and a certified <strong>Eppendorf</strong><br />
Specialist professional will handle it all. Extended Warranty<br />
and Advantage Service protect your hardware investment<br />
while any one of three Performance Plans will keep your<br />
system fine tuned and in compliance to deliver results you<br />
can count on.<br />
Service offers—ep realplex<br />
Performance Plans—ep realplex<br />
Order direct from <strong>Eppendorf</strong> <strong>Canada</strong> Ltd.<br />
Product appearance, specifications, and/or prices are subject to change without notice.<br />
Long-term benefits of performance plans:<br />
‡ Confidence in results<br />
‡ GLP compliance<br />
‡ Assurance of performance over extended lifetime<br />
Features of <strong>Eppendorf</strong> Performance Plans:<br />
‡ Complete satisfaction and quality delivered by<br />
<strong>Eppendorf</strong> experts<br />
‡ Complete programs—easy to understand,<br />
easy to budget, no surprises<br />
‡ Complete documentation management—<br />
everything you need to be in compliance<br />
Description Model Catalog No.<br />
Extended Warranty Mastercycler ep realplex 2-color system 950020927<br />
Mastercycler ep realplex 4-color system 950020935<br />
Advantage Repair Service Mastercycler ep realplex 2-color system 901520068<br />
Preventive<br />
Certification<br />
Description<br />
Maintenance Certification<br />
Plus<br />
Catalog No. 950033017 950033025 950033037<br />
Preventive Maintenance<br />
The Preventive Maintenance is carefully designed to keep<br />
the Mastercycler ep realplex in the best condition. The<br />
services include the following—cleaning of the instrument’s<br />
surface and block, a functional test of the thermoblock with<br />
a temperature validation, as well as a full system check with<br />
background calibration.<br />
Color Calibration<br />
A dedicated color calibration protocol is carried out by the<br />
service specialist. Based on this recalibration procedure the<br />
last issued certificate is updated.<br />
Validation Assay<br />
The validation assay is based on a qPCR run with<br />
specifically designed primers and DNA templates to cover<br />
all major performance aspects such as dynamic range and<br />
repro ducibility. Based on this validation protocol the last<br />
issued certificate is updated.<br />
Mastercycler ep realplex 4-color system 901520070<br />
‡ ‡ ‡<br />
— ‡ ‡<br />
— — ‡
Mastercycler® Systems Services<br />
Value of Service<br />
From a 2-year system warranty that comes with the unit,<br />
to Extended Warranties and Preventive Maintenance, we<br />
offer options that will keep your Mastercycler running for<br />
many cycles.<br />
Choose an Extended Warranty or Advantage Service to<br />
ensure optimal performance of your instrument while<br />
Preventive Maintenance will keep the system running<br />
smoothly for years to come.<br />
Advantage Repair Service<br />
An easy to use value packaged service option when a<br />
repair is needed for systems not covered under a warranty<br />
or extended warranty. All it takes is one call to our Service<br />
Center and for a single fee we will repair the instrument,<br />
complete a full preventive maintenance, repair anything<br />
else we find, plus include a full year warranty on the whole<br />
system, not just the repair. Instrument return shipment is<br />
also included.<br />
Service offers—Mastercycler systems<br />
Description Model Catalog No.<br />
Extended Warranty Mastercycler personal 901510551<br />
Mastercycler 901510553<br />
Mastercycler gradient 901510555<br />
Mastercycler ep/pro Manual lid 901510556<br />
Mastercycler ep Motorized lid 901510557<br />
Mastercycler nexus Please Inquire<br />
Advantage Repair Service Mastercycler 901520044<br />
Mastercycler gradient 901520046<br />
Mastercycler personal 901520048<br />
Mastercycler ep/pro Manual lid 901520050<br />
Mastercycler ep Motorized lid 901520052<br />
Mastercycler ep/pro S Manual lid 901520054<br />
Mastercycler ep S Motorized lid 901520056<br />
Mastercycler ep/pro 384 Manual lid 901520058<br />
Mastercycler ep 384 Motorized lid 901520060<br />
Mastercycler ep/pro Control Panel 901520061<br />
Performance Plans—Mastercycler Mastercycler systemsnexus<br />
Please Inquire<br />
Description Model Catalog No.<br />
Preventive Maintenance Mastercycler Systems 901510526<br />
Temperature Validation<br />
and Calibration<br />
Mastercycler Systems 901510697<br />
Sample Service products Handling 215<br />
In the U.S.: <strong>Eppendorf</strong> North America 800-645-3050 · In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715<br />
E-mail: info@eppendorf.com · www.eppendorf.com<br />
4 Services – PCR
Support and Services Directory<br />
Contact Information<br />
United States <strong>Canada</strong><br />
Business Hours: 8:30 a.m. to 8:00 p.m. EST 8:30 a.m. to 5:00 p.m. EST<br />
Phone: 800-645-3050<br />
516-334-7500<br />
800-263-8715<br />
905-826-5525<br />
Fax: 516-334-7506 905-826-5424<br />
Website: www.eppendorfna.com www.eppendorf.ca<br />
Email: info@eppendorf.com canada@eppendorf.com<br />
Customer Support: 800-645-3050<br />
custserv@eppendorf.com<br />
Technical Service: 800-645-3050<br />
techserv@eppendorf.com<br />
www.eppendorf.com • Email: info@eppendorf.com<br />
800-263-8715<br />
canadacustserv@eppendorf.com<br />
800-645-3050<br />
techserv@eppendorf.com<br />
In the U.S.: <strong>Eppendorf</strong> North America, Inc. 800-645-3050 • In <strong>Canada</strong>: <strong>Eppendorf</strong> <strong>Canada</strong> Ltd. 800-263-8715