Survival of the Fittest— - Eppendorf

4 A History of Advanced PCR
184 Sample Handling
Quality is timeless
Eppendorf ® is an innovator, designing instruments that
fit the needs of today’s scientist. The Mastercycler ®
gradient by Eppendorf—still popular today—was
the first available gradient thermal cycler. Today the
gradient function remains critical for the optimization
of a PCR reaction.
The next generation Mastercycler ep is a lab favorite
and still one of the fastest cyclers around. Given this
history of innovation, it’s only natural that Eppendorf
would now introduce the latest in evaporation reduction
technology—vapo.protect ® . With a shift to low volume
reactions and faster protocols, the need to safeguard
the integrity of your sample has become increasingly
vital. The Mastercycler pro does just that.
Survival of the Fittest—
In the age of networking, the new Mastercycler nexus is
your reliable companion when it comes to daily routine
PCR. With its ease-of-use, small footprint, energy saving
features and its’ capability to notify you by e-mail when it
is finished—what else do you need from a cycler?
Eppendorf has also addressed the needs of real-time
PCR. Integrating the advantages of the Mastercycler ep
gradient family, we have developed a fully licensed
real-time PCR system that meets the requirements
of the latest quantitative PCR applications.
A history of advanced PCR
‡ Microcycler: Eppendorf
introduces its first thermal
cycler using water to heat
and cool.
‡ Mastercycler 5330:
Eppendorf introduces the
first Mastercycler based
on peltier technology.
1990 1993
Faster speeds, highest precision, excellent temperature
control stability, extremely user-friendly, and absolute
reliability united with a flexible concept: these are the
characteristics of the Mastercycler families.
‡ Mastercycler gradient:
the first gradient thermal
cycler on the market.
‡ Thin-walled 0.2 mL
PCR tubes allow for
rapid heat transfer.
‡ Dual component twin.tec ®
plates provide a stable
frame and extremely
thin-walled wells for
optimum heat transfer.
1997 1998 2002

‡ Mastercycler ep:
Fast heating/cooling rates
and ultra quiet operation—
in a compact design.
2003 2005
The Mastercycler® Family
‡ Mastercycler ep
realplex: Extremely
fast optics for rapid
data acquisition.
Sample Handling 185
Then... Now—always providing quality solutions for your lab.
‡ Mastercycler pro:
New vapo.protect ®
technology reduces
evaporation. ‡ qPCR consumables
and divisible twin.tec
PCR plates.
‡ Mastercycler nexus:
Connects to network
to send status updates
through e-mail.
2008 2010 Today
4 A History of Advanced PCR

4 Summary of Applications
186 Sample Handling
Summary of applications
Application
Instruments
Product Page
Conventional PCR Mastercycler ® pro
188
Mastercycler nexus Family
192
Real-time PCR Mastercycler ep realplex 195
PCR optimization Mastercycler pro
188
Mastercycler nexus gradient
194
in situ PCR Mastercycler nexus
192
Mastercycler nexus gradient with in situ Adapter 212
Mastercycler nexus flat
194
Cycle Sequencing Mastercycler pro
188
Mastercycler nexus Family
192
Heat sealing of 96- and 384-well PCR plates
Consumables
Tubes, Plates and Accessories
Heat Sealer 208
PCR with sample throughput 0.2/0.5 mL PCR Tube, thin-walled 204
PCR with medium sample throughput 0.2 mL PCR Tubes in strips of 8
0.1 mL PCR Tubes in strips of 8
twin.tec ® 205
205
PCR Plate 96, unskirted, divisible 201
PCR with medium to high sample throughput twin.tec PCR Plate 96, semi-skirted
200
twin.tec PCR Plate 96, skirted
200
PCR with medium to high sample throughput,
usage in automatic devices
twin.tec PCR Plate 96, skirted 200
PCR with high sample throughput,
twin.tec PCR Plate 384
200
usage in automatic devices
twin.tec real time PCR Plate 384
206
Real-time PCR twin.tec real-time PCR Plates
206
real-time PCR Tube Strips
207
PCR with bacterial templates
Accessories
twin.tec microbiology PCR Plate 201
Non-cooled PCR Rack, work tray and frame 211
Cooled (0 °C and –21 °C) IsoTherm-System ® 124
Cooled (0 °C), color change for temperature control
Sealing options
PCR-Cooler 125
Partial seal for real-time PCR and low volume PCR Masterclear ® Cap Strips 207
Partial seal of PCR plates and PCR strips Cap Strips, Capping Aid 210
Heat sealing, transparent, non-removable,
cannot be pierced (e.g. for colorimetric evaluation)
Heat Sealing Film 208
Heat sealing, non-transparent, removable,
can be pierced
Heat Sealing Foil 208
Adhesive seal, transparent, removable,
cannot be pierced
PCR Film 209
Adhesive seal, transparent, removable,
cannot be pierced
PCR Foil 209
Adhesive seal, transparent, optically clear, removable, cannot be pierced Masterclear real-time PCR Film 210
4 | Info | PCR
Practice of the patented polymerase chain reaction (PCR) process requires a license. The Mastercycler is an Authorized Thermal Cycler and
may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in
accordance with the label rights accompanying such reagents.
This product is sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc. and Applied Biosystems.
Product appearance, specifications, and/or prices are subject to change without notice.

Summary of applications
Sample Handling 187
Application Product Page
Application tips for PCR
The table to the left shows a selection of particular applications from the
PCR area and serves as an overview. The complete product portfolio of
the Eppendorf ® PCR System is found on the following pages.
A selection of particular PCR products shown on the following pages, can
be provided as Customized Solutions. Further information on page 380.
Register your Eppendorf products online now to benefit from numerous
advantages! You receive bonus ep-points immediately for each Eppendorf
device registered. You can also easily manage your devices online and are
always kept up-to-date on any news.
epGreen ® Initiative
‡ Noise emission: Eppendorf PCR instruments are optimized
to minimize noise emission
‡ PCR consumables are made of virgin Polypropylene and
Polycarbonate that is fully recyclable
‡ All cardboard packaging is 100 % recyclable
‡ Wherever possible, water-soluble laquer or paints without
heavy metals are used
‡ Mini-Satellite-system: By controlling up to 5 units of the
Mastercycler ® pro with only 1 Control Panel, you can
increase your PCR capacity by only increasing the parts
needed, i.e. the thermo module. There is no need to buy
an entire additional system to increase your capacity
Mastercycler nexus, Mastercycler nexus gradient,
Mastercycler nexus eco
Purchase of this instrument conveys a limited non-transferable
immunity from suit for the purchaser’s own use under certain
non real-time thermal cycler patents licensed from Applied
Biosystems, LLC.
Mastercycler ep realplex
This is a Licensed Real-Time Thermal Cycler or Licensed
Real-Time Temperature Cycling Instrument under Applera’s
United States Patent No. 6,814,934 and corresponding claims
in non-U.S. counterparts thereof, for use in research and for all
other applied fields except human in vitro diagnostics. No right
is conveyed expressly, by implication or by estoppel under any
other patent claim.
customized
products
p. 380
‡ Connect up to 2 Mastercycler nexus eco units to your
existing unit to acheive low energy consumption
‡ Stand-by function of the Mastercycler nexus saves energy
For more information on epGreen, see pages 398 and 399.
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
Summary of Applications
4

Thermocyclers
4
188 Sample Handling
Mastercycler® pro
®
vapo.protect ® technology*
Evaporation in a PCR reaction vessel not only limits the ability
to work with low reaction volumes but also makes it difficult to
obtain reproducible results. For the Mastercycler pro it has been
Eppendorf’s aim to protect the PCR reaction from evaporation.
While common PCR instruments press a metal plate onto
the PCR consumable, the vapo.protect lid covers the PCR
consumable with a cushion that conforms to the shape of the
consumable—no matter which consumable you use. The snug
fit of the cushion in combination with the high pressure and
excellent heating capabilities of the lid reduce evaporation to
a minimum.
Evaporation leads to increasing concentrations of the PCR
master mix components, especially primers. This, in turn,
leads to non-specific binding. The changes in concentrations
due to evaporation both during the experiment and between
experiments can be a cause for great concern for any scientist
who tries to keep variables to a minimum.
Gradient with SteadySlope ® technology
The gradient option of the Mastercycler pro features
SteadySlope technology to ensure that the heating and
cooling ramp rates are identical in both gradient and normal
operation. Thus, you can expect identical temperature control
characteristics during both optimization and routine experiments.
Programming the gradient with the Control Panel or the
CycleManager pro is easy and intuitive—so that novice users
can get started quickly and safely.
*Patents pending
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
vapo.protect
technology leads to improved
reproducibility and specificity
at the rim and in the corners
of the block
Certified Quality
– Individual, documented quality control certificate
– Calibration according to national and international standards:
NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK)
4 | Instruments | PCR
Description
Fastest speeds, highest precision, easy to use and absolute
reliability combined in a flexible concept—that is the
Mastercycler pro.
The requirements for PCR are defined by research projects that
are continually evolving. As the needs of the researchers change
the instrument they use needs to be adaptable. That is why only
an open and flexible system fits into today’s lab. Eppendorf’s
Mastercycler pro offers ultimate flexibility:
– choose from three block formats: 96, 96 Silver and 384
– operate the Mastercycler pro in various configurations
– use any plate, tube or strip that you choose
Unsurpassed speed can be achieved with Eppendorf’s Impulse
PCR, a device driven hot-start function that increases heating
rates to 8 °C/s.
For additional information, see application tips for PCR
on page 393.

Mastercycler® pro
Technical specifications
Sample Handling 189
Description Mastercycler pro Mastercycler pro S Mastercycler pro 384
Thermoblock Aluminum Silver Aluminum
Sample capacity 96 x 0.2 mL PCR tubes or 1 PCR plate 8 x 12 (unskirted,
semi-skirted, skirted—according to SBS standard)
Temperature control range of the block 4–99 °C 4–99 °C 4–99 °C
1 PCR plate 384
Temperature control mode Fast, Standard, Safe ; all available in gradient mode
Heating technology of the block Peltier Elements, Triple Circuit Technology
Gradient block Over 12 columns Over 12 columns Over 24 columns
Gradient range 1–20 °C 1–24 °C 1–20 °C
Gradient temperature range 30–99 °C 30–99 °C 30–99 °C
Lid temperature range 37–110 °C 37–110 °C 37–110 °C
Lid descent and closing pressure vapo.protect ® technology, with Thermal Sample Protection
Block homogeneity 20–72 °C ±0.3 °C,
90 °C ±0.4 °C
Control accuracy ±0.2 °C ±0.2 °C ±0.2 °C
Heating rate* approx. 4 °C/s approx. 6 °C/s approx. 4 °C/s
Cooling rate* approx. 3 °C/s approx. 4.5 °C/s approx. 3 °C/s
Interfaces 1 x Centronics ® , 1 x RS-232, Control panel, one each of CAN_in/CAN_out
Dimensions (W x D x H) 10.2 x 16.3 x 14.6 in/
26 x 41.5 x 37 cm
Product features
‡ Extremely fast heating and cooling rates—
get results faster and do more in one day
‡ Ultimate reduction of evaporation for reliable results
‡ Gradient blocks with SteadySlope ® technology for
protocol optimization
‡ Intuitive graphic user interface
‡ Display to indicate cycler number in a network
‡ Upgradable to real-time PCR
‡ 2-year warranty
‡ Optional self-test dongle available to test functionality
of the Peltier Elements
‡ Stand alone system fits on virtually every lab bench
and is operated by a control panel
‡ Expandable to a Mini Satellite System or network
– Mini Satellite System: operated by Control Panel.
Control up to 5 units with one Control Panel.
Combine any block format. Expandable to a network.
– Computer Network: combines up to 30 units for
ultimate throughput. Operated by a computer and the
CycleManager pro software. Benefit from a client/
server architecture and full software functionality.
10.2 x 16.3 x 14.6 in/
26 x 41.5 x 37 cm
10.2 x 16.3 x 14.6 in/
26 x 41.5 x 37 cm
Weight 40.8 lbs /18.5 kg 40.8 lbs /18.5 kg 40.8 lbs /18.5 kg
Power supply 120 V, 50–60 Hz 120 V, 50–60 Hz 120 V, 50–60 Hz
Power consumption 950 W 950 W 950 W
* Block temperature
This product is licensed under U.S. patent Nos. 5,525,300, 5,779,981 and 6,054,263. The heated cover device is licensed under US 5,552,580 and foreign equivalents. The user of the Eppendorf Mastercycler pro
might require additional rights for kits, reagents and other components required for his/her application. Such accompanying rights for these kits, reagents and other may be obtained by the respective holder
of such rights. No rights are conveyed expressly, by implication or estoppel to any patents on real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent or kit.
Mastercycler pro upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts.
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 Thermocyclers

Thermocyclers
4
190 Sample Handling
Mastercycler® pro
Control panel programming features
‡ Highest degree of flexibility, graphical user interface
‡ Variable ramp rates
‡ Simulation mode for temperature control speed and
protocols of the original Mastercycler family—
heating 3 °C/s, cooling 2 °C/s
‡ Time and temperature increments
‡ Pause command
‡ Programmable alarm sound
‡ Freely configurable auto-restart dialogue
Regulatory documentation
Compliance with GMP/GLP guidelines is becoming increasingly
important, particularly in the pharmaceutical and biotechnology
industries. The Mastercycler pro system’s programming and user
management system provides a GLP-conforming environment
for your PCR assays.
Technical specifications of the Control Panel
Number of Mastercycler pro cyclers that can be controlled 1–5
Memory capacity 16 MB, >700 programs
Memory expansion MultiMediaCard reader and 2 x USB port 2.0
Number of user-defined folders >100
Dimensions (W x D x H) 10.0 x 6.6 x 4.4 in/25 x 16.5 x 11 cm
Interfaces 2 x USB port
Weight 2.6 lb/1.2 kg
Power supply, consumption Via the connected thermal module
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Program and user administration features
‡ One administrator to manage user and service functions
‡ Easy addition of new users with limited rights
‡ Ability for Mastercycler pro users to change their
passwords; no administrator access to user passwords
‡ Password protection against unauthorized login
‡ A report file for each run, including total temperature
profiling
‡ Development, testing and validation of the software
using certified standards
This product is licensed under U.S. patent Nos. 5,525,300, 5,779,981 and 6,054,263. The heated cover device is licensed under US 5,552,580 and foreign equivalents. The user of the Eppendorf Mastercycler pro
might require additional rights for kits, reagents and other components required for his/her application. Such accompanying rights for these kits, reagents and other may be obtained by the respective holder of such
rights. No rights are conveyed expressly, by implication or estoppel to any patents on real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent or kit. Mastercycler pro
upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts.

CycleManager pro for Mastercycler® pro
Ordering information
Product features
Sample Handling 191
‡ Network and control up to 30 Mastercycler pro
thermal cyclers with a PC; mix and match models
of choice
‡ Multi-Mastercycler-Networking with a PC
‡ Intuitive Windows ® -like interface
‡ Icon-based creation and edit of programs
‡ Simultaneous control of all or selected
Mastercycler pro cyclers
‡ GLP-conformity documentation of all PCR experiments
‡ User password protection
‡ Real-time acquisition of temperature data
‡ Easy installation and uninstallation
‡ Compatible with Windows
‡ Easy transfer of PCR programs from the Control Panel
to the PC
Description
Complete Mastercycler pro package (includes cycler and control panel)
Catalog No. List USD
Mastercycler pro and Control Panel, 120 V, 50–60 Hz 950040015 $9,057.00
Mastercycler pro S and Control Panel, 120 V, 50–60 Hz 950040025 9,845.00
Mastercycler pro 384 and Control Panel, 120 V, 50–60 Hz
Individual Mastercycler pro modules
950040035 9,549.00
Control Panel, includes connection cable 950030050 2,229.00
Mastercycler pro*, 120 V, 50–60 Hz 950030010 8,063.00
Mastercycler pro S*, 120 V, 50–60 Hz 950030020 8,958.00
Mastercycler pro 384*, 120 V, 50–60 Hz
Accessories
950030030 8,622.00
CAN_Bus connection cable, 50 cm 950014008 42.00
CAN_Bus connection cable, 150 cm 950014016 52.00
Self Test Dongle 950030040 308.00
MultiMediaCard, 64 MB 960002008 90.00
CycleManager pro, incl. installation manual, online help, and connection cable
Service
Extended Warranty, see page 215 for details
Preventive Maintenance, see page 215 for details
950017007 2,346.00
* A Control Panel or CycleManager pro software (both sold separately) is required for operation; CAN_Bus connection cables are required to link cyclers together as a network.
This product is licensed under U.S. patent Nos. 5,525,300, 5,779,981 and 6,054,263. The heated cover device is licensed under US 5,552,580 and foreign equivalents. The user of the Eppendorf Mastercycler pro
might require additional rights for kits, reagents and other components required for his/her application. Such accompanying rights for these kits, reagents and other may be obtained by the respective holder of such
rights. No rights are conveyed expressly, by implication or estoppel to any patents on real-time methods, including but not limited to 5‘ nuclease assays, or to any patent claiming a reagent or kit. Mastercycler pro
upgraded to a Mastercycler ep realplex requires a Real-Time Thermal Cycler License under Applera’s United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts.
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 Thermocyclers

Thermocyclers
4
192 Sample Handling
Mastercycler® nexus
NEW!
‡ Stand-by function saves energy
‡ Graphical user interface
‡ USB port for data transfer
or mouse connection
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
‡ E-mail notification

Mastercycler® nexus
‡ One hand operation
‡ flexlid adjusts to
height of consumables
‡ Connect up to 2 Mastercycler nexus eco
units to increase throughput
Sample Handling 193
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 Thermocyclers

Thermocyclers
4
194 Sample Handling
Mastercycler® nexus
Description
The Mastercycler nexus offers the reliability and ease-of-use
of an Eppendorf cycler with low energy consumption, easy
expandability to 3 units and a small footprint make it not only fit
to your budget, but also to your precious lab space. It can handle
all kinds of PCR consumables, from low volume all the way up
to 0.5 mL PCR tubes. All common PCR plates fit—and if your
throughput becomes even higher, you can just connect 1 or
2 Mastercycler nexus eco to your Mastercycler nexus.
Product features
‡ Flexlid concept allows use of different consumables
with automatic height adjustment of the heated lid
‡ Intuitive graphic programming
‡ Small footprint
‡ Universal block for ultimate flexibility
‡ Connect up to 2 Mastercycler nexus eco units to your
existing unit
‡ Available with 12 column gradient
‡ E-mail notification
‡ Average PCR run power consumption of 0.154 kW/h
Further information under
www.eppendorfna.com/mastercyclernexus
For additional information see “Application tips for PCR”
on page 393.
Ordering information
Description Catalog No. List USD
Mastercycler nexus gradient, 120 V, 50/60 Hz 6331000025 $8,100.00
Mastercycler nexus, 120 V, 50/60 Hz 6333000022 7,200.00
Mastercycler nexus eco 1 , 120 V, 50/60 Hz 6332000029 5,500.00
Mastercycler nexus flat block, 120 V, 50/60 Hz 6335000020 7,400.00
Self test dongle 950030040 308.00
CAN-Bus connection cable 1 , 50 cm 950014008 42.00
CAN-Bus connection cable 1 , 150 cm 950014016 52.00
1 Requires Mastercycler nexus gradient or Mastercycler nexus to operate.
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Gradient with SteadySlope ® Technology
The gradient option of the Mastercycler nexus features the
SteadySlope technology to ensure that the heating and
cooling ramp rates are identical in both gradient and normal
operation. Thus, you can expect identical temperature control
characteristics in both optimization and routine experiments.
Programming the gradient is easy and intuitive – so that novice
users can get started quickly and safely.
Easy testing
The self test function of the Mastercycler nexus provides the
ability to check the peltier elements of the thermal block easily.
This feature tests the functionality of all six peltier elements and
provides a corresponding certificate. This way, the user can
document the homogeneity of the instrument between regular
testing intervals. In addition, Eppendorf ® offers a Temperature
Validation System as an external testing device.
Certified Quality
‡ Individual, documented quality control certificate
‡ Calibration according to national and international standards:
NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK)
‡ UL/CUL listed
High quality consumables
Consumables of high quality, e.g. Eppendorf twin.tec ® PCR
plates, help to improve PCR results. For example, the raised rims
of the Eppendorf twin.tec PCR plates are optimized to guarantee
tight sealing in all wells. For further information, please visit
www.eppendorfna.com/pcr

Mastercycler® ep realplex
Product features
High-speed real-time PCR
‡ Load your plate and receive analyzed results in less than
half an hour!
‡ Fast and easy programming
‡ Fast optical detection capabilities eliminate the need
for lengthy extension/elongation
‡ Aluminum block model offers faster ramp rates than
nearly any other supplier
‡ “S” model for highest speed
‡ Complete more experiments per day
No restrictions
‡ Use the reagents, kits and consumables of your choice
‡ Even the high-speed “S” model does not lock you in to
special kits or plates
Gradient
‡ Optimize your protocols in a single plate/experiment
‡ Test more distinct temperatures at once with Triple
Circuit thermal block design
Application
* New! Modified slip cover (light-transmissive cover for the optical openings of the heated plates assembly, US patent pending).
Licensed and authorized for real-time PCR; see next page.
‡ Quantitative real-time PCR
96-well
silver block
Sample Handling 195
96-well
aluminum block
Description
Continuing the Eppendorf ® focus on best design, the
Mastercycler ep realplex combines the highest performance
with the most advanced features and capabilities of a 96-well
real-time PCR system—and in a compact class!
Highly sensitive optics
‡ The new optical system of the Mastercycler ep realplex
offers a new, exclusive Particle Protection* and
Photomultipliers of the latest generation
Highest reproducibility
‡ LEDs keep consistent intensity over time—other light
sources lose intensity over time
‡ Each LED is normalized to equal intensity
‡ Over 55 years of Eppendorf expertise in optical systems
Intuitive software
‡ All analysis modules included (see pages 197 and 198)
‡ Highly accurate calculation of Ct-values using
the CalQplex ® algorithm
‡ “QUICK START” capability
Eppendorf design
‡ Quiet operation, 554 dB(A)
‡ Small footprint
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 Thermocyclers

Thermocyclers
4
196 Sample Handling
Mastercycler® ep realplex
Complete real-time PCR system twin.tec ® real-time PCR plate
96 individual LED array realplex filter wheels
Mastercycler ep realplex 2 and realplex 2 S
‡ These two-channel systems meet the needs of most
laboratories (emission 520/550 nm)
‡ Designed for use with SYBR ® Green assays,
FAM-labeled probes or single and duplex
probe reactions
‡ High reproducibility is possible at 10 μL total
reaction volumes with cali brated Eppendorf ® pipettes;
for lower volumes please inquire about automated
real-time PCR set-up
‡ 2-year warranty comes standard—best in its class!
‡ Lifetime warranty on LED array
Mastercycler ep realplex 4 and realplex 4 S
‡ This four-channel system (emission 520/550/580/605 nm)
provides full flexibility for multiplexing
‡ High reproducibility is possible at 10 μL total
reaction volumes with cali brated Eppendorf pipettes;
for lower volumes please inquire about automated
real-time PCR set-up
‡ 2-year warranty comes standard—best in its class!
‡ Lifetime warranty on LED
Practice of the patented polymerase chain reaction (PCR) process requires a license. The Eppendorf [or appropriate trademark] Thermal Cycler is an Authorized
Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in
accordance with the label rights accompanying such reagents. This is a Licensed Real-Time Thermal Cycler under Applera’s United States Patent No. 6,814,934 and
corresponding claims in non-U.S. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed
expressly, by implication or by estoppel under any other patent claim.
The purchase of this product includes a limited, non-transferable Iicense under U.S. Patent No. 5,871,908 and all continuations and divisionals, and corresponding
claims in patents and patent applications outside the United States, owned by Roche Diagnostics GmbH, for internal research use or for non-in vitro diagnostics
applications with authorized reagents with regard to Melting Curve Analysis. No right is conveyed, expressly, by implication or estoppel, under any other patent or
patent claims owned by Roche Diagnostics GmbH, or by any other Party.
Order direct from Eppendorf North America.
Product appearance, specifications, and/or prices are subject to change without notice.

Mastercycler® ep realplex
Eppendorf’s Mastercycler ep realplex software is intuitive and
user-friendly. The Windows ® -like interface allows for a clear
and comprehensive organization of multiple viewing windows.
The Plate layout screen is designed like a 96-well PCR plate
making labeling and identification easy. This screen also
provides a helpful “Hint” box in the bottom corner making
set up simple for users of any level. Once your experiment
has begun the Monitoring screen allows you to visualize the
results in real-time as they appear, and you can interrupt the
run once the desired results have been obtained!
‡ Plate layout
The clear and comprehensive organization of
multiple viewing windows, as well as intuitive
options for selection within each view, enable
easy and fast plate setup when creating assays.
‡ Quantification/relative quantification
This analysis module performs calculations
from raw data for a variety of assays, including
absolute quantification of DNA or relative
quantification of gene expression based
on the ADCt method.
Sample Handling 197
The Mastercycler ep realplex software is designed to be
intuitive for real-time PCR users of all levels, making data
collection and analysis easy, efficient and comprehensive.
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 | Instruments | PCR
‡ Raw data
This software module displays the generated
raw data in real-time, which means that you
can immediately evaluate your real-time PCR
and interrupt the reaction as soon as the
desired result is obtained.
‡ Gene identification
Selecting this analysis module option generates
allele-discrimination results. Data previously
generated in melting curve analyses, endpoint
measurements or Ct-value determinations
serves as the basis.
4 Thermocyclers

Thermocyclers
4
198 Sample Handling
Mastercycler® ep realplex
‡ Endpoint
The analysis module option for endpoint
measurements instructs Mastercycler ep
realplex to serve as a “plate fluorometer”
and aids in the determination of absolute
fluorescence intensities, even with samples
previously amplified on standard thermocyclers.
‡ +/– Assay
Define critical threshold values through this
analysis module option to differentiate between
positive and negative samples (e.g., for the
detection of pathogens). Data from previous
endpoint measurements serves as the source
for such determinations.
The Mastercycler ep realplex software includes various analysis modules making this system versatile and ideal
for multiple applications. Protein characterization can now be added to this list; with the use of our “Inverted Data”
tool all protein reactions can be easily evaluated with our system (see images below).
‡ Protein Characterization
Inverted data flips the negative peaks so the threshold is no longer below zero and the data may be properly evaluated.
Licensed for real-time PCR, see page 196.
Order direct from Eppendorf North America.
Product appearance, specifications, and/or prices are subject to change without notice.

Mastercycler® ep realplex
Technical specifications
Optical module
Excitation source 96 LEDs (470 nm)
Emission filters 520/550 nm (realplex2 )
520/550/580/605 nm (realplex4 )
Detector 1 photomultiplier tube (realplex2 )
2 photomultiplier tubes (realplex4 )
Dynamic range 8 orders of magnitude from starting copy number
Sensitivity
Thermomodule
≤50 fmol fluorescein
Sample capacity 96 x 0.2 mL PCR tubes or one 96-well PCR plate
(unskirted, semi-skirted, skirted—as per SBS format)
Temperature control range of block 4–99 °C
Degree range of gradient, maximum 1–20 °C (aluminum block)
1–24 °C (silver block)
Temperature control range of gradient 30–99 °C
Temperature of lid 105 °C
Block homogeneity 20–72 °C ±0.3 °C,
90 °C ±0.4 °C
Control accuracy ±0.2 °C
Heating speed* approx. 4 °C/s (aluminum block)
approx. 6 °C/s (silver block)
Cooling speed*
Complete system
approx. 3 °C/s (aluminum block)
approx. 4.5 °C/s (silver block)
Dimensions (W x D x H) 10.24 in x 16.14 in x 15.59 in/26 cm x 41 cm x 39.6 cm
Total weight 52.91 lb/24 kg
Voltage requirements 115 V, 50 Hz–60 Hz
Power consumption 800 W
* Measured at block
Ordering information
Description Catalog No.
Mastercycler ep realplex
Mastercycler ep realplex2 , with aluminum block and two emission filters Please Inquire
Mastercycler ep realplex2 S, with silver block and two emission filters Please Inquire
Mastercycler ep realplex4 , with aluminum block and four emission filters Please Inquire
Mastercycler ep realplex4 S, with silver block and four emission filters Please Inquire
System accessories
Real-time PCR consumables, see page 206 or visit www.eppendorfna.com
Service
Performance plans, see page 214 for details
Please contact Eppendorf North America at 800-645-3050 to receive a quotation for the Mastercycler ep realplex system.
Sample Handling 199
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 Thermocyclers

4 PCR Plates
200 Sample Handling
twin.tec® PCR Plates
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
4 | Consumables | PCR
General product features
‡ One-piece design: combining a polycarbonate frame
and polypropylene wells for optimum performance
‡ Extremely thin-walled polypropylene wells guarantee
optimum heat transfer to the sample
‡ Exceptionally solid and torque-resistant
polycarbonate frame
‡ Improved well-to-well tolerance
‡ Minimal binding of DNA, RNA and enzymes
‡ Cut-away corner and alphanumeric grid-referencing
‡ Raised well rims for effective sealing, also reduces
risk of cross-contamination
‡ Autoclavable (121 °C, 20 min)
‡ Certified free of any detectable human DNA, DNase,
RNase and PCR inhibitors*
‡ Available with barcode (upon request)
‡ Ideal for quantitative real-time PCR as well
* Certificate, test procedures and detailed information available on request.
Additional features of twin.tec PCR Plate 96,
skirted and semi-skirted
‡ Compatible with automated systems
‡ Skirted for label or bar code
‡ Fits most thermal cyclers and the MegaBACE ®
capillary sequencer
‡ Within SBS footprint recommendations
(127.76 x 85.47 mm ±0.25 mm)
‡ Stackable
‡ Eight holes in the skirt aid plate positioning and removal
from the thermal cycler block
‡ Low profile design enhances efficiency of PCR and
enables the highest efficiency for small sample volumes
‡ 150 μL max. well volume (when used with cap strips, skirted)
‡ 250 μL max. well volume (when used with cap strips,
semi-skirted)
Additional features of twin.tec PCR Plate 384
‡ Continuous skirt for compatibility with
automated systems
‡ Skirted for label or bar code
‡ Stackable
‡ Within SBS footprint recommendations
‡ Ideal for most common 384-well thermal cyclers,
particularly for Eppendorf Mastercycler ® pro 384
(see page 189)
‡ 45 μL max. well volume
For more information visit www.eppendorfna.com/twintecplates

twin.tec® PCR Plates
Sample Handling 201
Product features of twin.tec PCR Plate 96, unskirted
‡ Fits on a wide variety of thermal cyclers
‡ OptiTrack ® matrix: high contrast labeling of alphanumeric
grid for easier sample tracking
‡ Available with regular (250 μL) or low profile (150 μL) wells
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 | Consumables | PCR
Product features of twin.tec PCR Plate 96, unskirted,
divisible
‡ Can be divided in 4 segments of 24 wells each
‡ Fits on a variety of thermal cyclers
‡ OptiTrack matrix: high contrast labeling of alphanumeric
grid for easier sample tracking
‡ Available with regular (250 μL) or low profile (150 μL) wells
Product features of twin.tec microbiology PCR Plate
‡ Sterile, individually blistered plates
‡ Additionally free of bacterial DNA
‡ For all applications that need ultimate purity or certified
absence of bacterial DNA
4 PCR Plates

4 PCR Plates
202 Sample Handling
twin.tec® PCR Plates
Technical specifications. All dimensions in millimeters.
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.

twin.tec® PCR Plates
Ordering information
* Eppendorf owns protective rights under European Patent EP 1 161 994 and US patents US 7,347,977 and US 6,340,589
For more information on Eppendorf purity levels, see pages 394 to 397, or visit www.eppendorfna.com/purity.
Sample Handling 203
Description Catalog No. List USD
twin.tec PCR Plate 96*, skirted (clear wells), 25 pcs., (150 μL max. volume)
Clear frame 951020401 $107.00
Yellow frame 951020427 107.00
Green frame 951020443 107.00
Blue frame 951020460 107.00
Red frame
twin.tec PCR Plate 96*, skirted (black wells), 25 pcs., (150 μL max. volume)
951020486 107.00
Yellow frame
twin.tec PCR Plate 96*, semi-skirted (colorless wells), 25 pcs., (250 μL max. volume)
951020508 107.00
Clear frame 951020303 107.00
Yellow frame 951020320 107.00
Green frame 951020346 107.00
Blue frame 951020362 107.00
Red frame
twin.tec PCR Plate 384* (colorless wells), 25 pcs., (45 μL max. volume)
951020389 107.00
Clear frame 951020702 209.00
Yellow frame 951020711 209.00
Green frame 951020729 209.00
Blue frame 951020737 209.00
Red frame
twin.tec PCR Plate 96, unskirted low profile, 20 pcs., (150 μL max. volume)
951020745 209.00
Clear frame 0030133307 79.00
Yellow frame 0030133315 79.00
Green frame 0030133323 79.00
Blue frame 0030133331 79.00
Red frame 0030133340 79.00
Clear frame, divisible 0030133358 157.00
Blue frame, divisible
twin.tec PCR Plate 96, unskirted, 250 µL, 20 pcs., (250 μL max. volume)
0030133382 157.00
Clear frame 0030133366 79.00
Blue frame 0030133390 79.00
Clear frame, divisible 0030133374 157.00
Blue frame, divisible
twin.tec microbiology PCR Plate 96, skirted, 10 pcs., (150 μL max. volume)
0030133404 157.00
Clear frame 0030129300 79.00
Blue frame
twin.tec microbiology PCR Plate 96, semi-skirted, 10 pcs., (250 μL max. volume)
0030129318 79.00
Clear frame 0030129326 79.00
Blue frame
twin.tec microbiology PCR Plate 384, 10 pcs., (45 μL max. volume)
0030129334 79.00
Clear frame 0030129342 116.00
Blue frame 0030129350 116.00
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 PCR Plates

4 PCR Tubes
204 Sample Handling
PCR Tubes
More space for labeling due to improved lid design
Fig. 1: 0.5 mL PCR Tube
writing surface
Product features of 0.5 mL PCR Tubes, thin-walled Product features of 0.2 mL PCR Tubes, thin-walled
‡ Special space-saving lid design
‡ For maximum capacity in the universal blocks of
Eppendorf Mastercyclers ® and other 0.5 mL blocks
‡ High transparency
‡ Etched lid for labeling
‡ Tight sealing
‡ Easy to open
‡ For use with all thermal cyclers with 0.5 mL block format
‡ Certified free from human DNA, DNase, RNase and
PCR inhibitors*
Description
Original Eppendorf ® 0.2 mL and 0.5 mL PCR Tubes are
manufactured to the highest quality standards. These thin-walled
polypropylene tubes ensure efficient and homogeneous heat
transfer of the sample, thanks to their even wall thickness and
smooth wall surface. Heat-sealed bags of tubes are enclosed in
boxes that are also sealed with a PCR clean sticker to ensure the
highest degree of purity.
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Contamination shield prevents contact with inner lid surface
Fig. 2: 0.2 mL PCR Tube
writing surface
‡ Contamination shield on hinged lid
‡ Defined lid position due to special hinge
‡ High transparency even on the tube base
‡ Etched lid for labeling
‡ Tight sealing
‡ Easy to open
‡ For use with all thermal cyclers with 0.2 mL block format
‡ Also available in 8-tube strip format
‡ Certified free from human DNA, DNase, RNase and
PCR inhibitors*
* Certificate, test procedures and detailed information available on request.
defined lid position due
to special hinge

PCR Tubes
Ordering information
Product features of 0.1 mL PCR Tube Strips
‡ 8 reaction tubes in strip-format —ideal for small
sample volumes
‡ Easily adaptable for automation
‡ Sealable using flat or domed strip caps
‡ Certified free from human DNA, DNase, RNase
and PCR inhibitors*
* Certificate, test procedures and detailed information available on request.
Sample Handling 205
Description
0.1 mL Eppendorf
Catalog No. List USD
® PCR Tubes
PCR Tube Strips 0.1 mL, without lids (10 × 12 strips) 0030124804 $118.00
PCR Tube Strips 0.1 mL plus Cap Strips, domed (10 × 12 strips each) 0030124812 176.00
PCR Tube Strips 0.1 mL plus Cap Strips, flat (10 × 12 strips each)
0.2 mL Eppendorf PCR Tubes
0030124820 182.00
Clear, 1,000 pcs. 951010006 136.00
8-tube strip, for 0.2 mL PCR Tubes, pack of 120 (960 tubes total), with dome hinged cap
0.5 mL Eppendorf PCR Tubes, thin-walled with hinged lid
951010022 185.00
Clear, 500 pcs. 0030124537 67.00
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 PCR Tubes

4 PCR Plates
206 Sample Handling
twin.tec® real-time PCR Plates
Product features
‡ White wells for better reflection
‡ High mechanical stability makes them torque-resistant
‡ Raised rims for effective sealing
‡ “Skirted” (stackable) and “semi-skirted” plates
‡ Optimal heat transfer due to reduced wall thickness
‡ Autoclavable (121 °C, 20 min)
‡ Certified free of any detectable human DNA, DNase,
RNase and PCR inhibitors*
‡ Available with barcode (upon request)
* Certificate, test procedures and detailed information available on request.
Reflection is up to 10-fold higher than with frosted wells.
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Description
Eppendorf ® twin.tec real-time PCR Plates 1 offer all the advantages
of regular twin.tec plates and give you the advantage of white
wells for your real-time PCR. The limiting factor in low volume
real-time PCR is often the remaining intensity of fluorescence.
The white wells of the Eppendorf twin.tec real-time PCR plates
reflect fluorescence much better than clear or frosted wells.
Thus, lower levels of fluorescence are still detectable with
the same instrument—just by using the right consumables.
Additionally, white wells significantly reduce interfering
background fluorescence and lead to increased homogeneity
of replicates and reproducible results. The rigidity of the
polycarbonate frame ensure easy and reliable handling—
manually or automated. The polypropylene wells guarantee
excellent and fast temperature transfer to the sample.
1 Eppendorf owns protective rights under European Patent EP 1 161 994 and US patents US 7,347,977
and US 6,340,589
For more information visit www.eppendorfna.com/realtime

twin.tec® real-time PCR Plates
Ordering information
For more information on Eppendorf purity levels, see pages
394 to 397, or visit www.eppendorfna.com/purity.
Masterclear® Cap Strips and real-time PCR Tube Strips
Ordering information
Product features
Masterclear Cap Strips
Sample Handling 207
Description Catalog No. List USD
twin.tec 96 real-time PCR Plates, skirted, set of 25 pcs.
White frame 951022015 $152.00
Blue frame 951022003 152.00
Black frame
twin.tec 96 real-time PCR Plates, semi-skirted, set of 25 pcs.
951022027 152.00
White frame 951022055 152.00
Blue frame 951022043 152.00
Black frame
twin.tec 96 real-time PCR Plates, unskirted low profile, set of 20 pcs. (150 μL max. volume)
951022067 152.00
White frame 0030132700 94.00
Blue frame 0030132718 94.00
Black frame
twin.tec 384 real-time PCR Plates, skirted, set of 25 pcs.
0030132726 94.00
White frame 0030132734 236.00
Blue frame 0030132742 236.00
Black frame 0030132750 236.00
‡ Inverted dome to avoid scratching of the optical surface
‡ Inverted dome to reduce dead volume of the tubes
‡ Optimized for ultimate light transmission
‡ Optically clear for real-time PCR
real-time PCR Tube Strips
‡ White wells for better reflection—ideal for use with lower
sample volume
‡ High mechanical stability
‡ Extremely thin walls for optimal heat transfer
‡ 0.1 mL Tube Strips
Description Catalog No. List USD
Masterclear Cap Strips, 10 x 12 pieces 951022089 $ 92.00
real-time PCR Tube Strips, without caps, 10 x 12 pieces 951022102 157.00
Masterclear Cap Strip and real-time PCR Tube Strip, 10 x 12 pieces each 951022109 246.00
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 PCR Plates

4 Heat Sealing Materials
208 Sample Handling
Heat Sealer
Heat sealing materials
Product features
‡ Hermetic heat sealing of multiwell plates
‡ Eliminates evaporation in PCR, reducing
cross-contamination
‡ Ideal for sample transportation and storage
‡ Seals 96- and 384-well plates
‡ Suitable for plates of different heights
‡ Optimum sealing with Eppendorf ® Heat Sealing Foils
and Films at a preset temperature
‡ Compact design, portable device
‡ Integrated thermostat prevents overheating
‡ Heating plate faces away for safety
‡ Improved mechanics for even easier sealing
Product features
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
Ordering information
Description Catalog No. List USD
Heat Sealer, 115 V/50 Hz 951023078 $2,032.00
Base plate, for 384-well plate 951023086 425.00
‡ Hermetic sealing of multiwell plates, especially
recommended for low reaction volumes
‡ Best protection against evaporation during PCR
‡ Certified free of human DNA, DNase, RNase and
PCR inhibitors*
‡ PCR foil is both pierceable and peelable
‡ Maximum adhesiveness at high temperature during PCR
* Certificate, test procedures and detailed information available upon request.
Ordering information
Description Catalog No. List USD
Heat Sealing PCR Film, 100 pcs. 0030127838 $194.00
Heat Sealing PCR Foil, 100 pcs. 0030127854 127.00

Heat sealing materials
Technical specifications
Description Heat Sealing PCR Film Heat Sealing PCR Foil
Packaging unit 1 x 100 pcs. 1 x 100 pcs.
Features
‡ Optically clear polyester/
polypropylene laminate
‡ Extremely stable sealing option —
cannot be removed or pierced
‡ Laminated aluminium foil
‡ Easily removable
‡ Easily pierceable, even with
multichannel pipettes
Sample Handling 209
Seal integrity –80 °C to 100 °C –80 °C to 100 °C
Sealing time with
Eppendorf ® Heat Sealer
4 sec. 4 sec.
Weldable materials
‡ Polypropylene
‡ Polypropylene
Special applications
‡ Colorimetric applications
‡ Recommended for use in automated
‡ Fluorescence applications,
including real-time PCR
‡ Storage of hazardous samples
work processes
PCR Film (self-adhesive) and PCR Foil (self-adhesive)
Product features
‡ Effective adhesive sealing prevents evaporation loss
during reaction and storage
‡ Removal from the plate with no residue
‡ Certified free of human DNA, DNase, RNase and
PCR inhibitors*
PCR Film
‡ Sample can be visually monitored through the
transparent film
PCR Foil
‡ Easily pierced
‡ No sticking of the pipette tip (ideal for automated systems)
* Certificate, test procedures and detailed information available upon request.
Ordering information
Description Catalog No. List USD
PCR Film (self-adhesive),
100 pcs.
0030127811 $187.00
PCR Foil (self-adhesive),
100 pcs.
0030127820 172.00
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 Heat Sealing Materials

4 Heat Sealing Materials
210 Sample Handling
Masterclear® real-time PCR Film
Cap Strips
Capping Aid
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
4 | Consumables | PCR
Product features
‡ Optimized for maximum light transmission
‡ Effectively forms a tight seal to reduce evaporation
‡ Maximum handling comfort due to optimized packaging
‡ Optically clear for real-time PCR
Ordering information
Description Catalog No. List USD
Masterclear real-time PCR Film,
(self-adhesive), 100 pieces
951022115 $245.00
Product features
‡ Strips with eight microcaps for 0.1 and 0.2 mL wells
‡ Easy and rapid sealing of Eppendorf ® twin.tec ® PCR
Plates, PCR strips and other standard multiwell plates
‡ Flat Cap Strips are suitable for real-time PCR
‡ Autoclavable (121 °C, 20 min)
Ordering information
Description Catalog No. List USD
Cap Strips (8-strips),
domed, 10 x 12 pcs.
0030124839 $67.00
Cap Strips (8-strips),
flat, 10 x 12 pcs.
0030124847 74.00
Product features
‡ Tool for easy and fast sealing of 0.2 mL PCR test tubes
and twin.tec PCR Plates with Cap Strips
‡ Several wells and tubes can be sealed simultaneously
‡ Minimal force required for an effective seal
‡ Minimizes losses due to evaporation
‡ Certified free of human DNA, DNase, RNase and
PCR inhibitors*
Ordering information
Description Catalog No. List USD
Capping Aid, 1 pc. 951023108 $22.00
* Certificate, test procedures and detailed information available upon request.

Work tray and frame
PCR Rack
Product features
Sample Handling 211
‡ Frame with work tray serves as a 2-part polycarbonate
rack for 96 x 0.2 mL PCR tubes
‡ All tubes can be directly transferred to a thermoblock
‡ Autoclavable (121 °C, 20 min)
Ordering information
Description Catalog No. List USD
Work tray, for 96 x 0.2 mL
PCR tubes, set of 10
951010031 $45.00
Frame for work tray, set of 5 951010049 41.00
Product features
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 | Consumables | PCR
‡ Rack for handling and storing tubes and plates
‡ For 0.2 mL, 0.3 mL and 0.5 mL tubes, as well as
96-well plates
‡ Stackable
‡ Can be stored down to –90 °C
‡ Autoclavable (121 °C, 20 min)
Ordering information
Description Catalog No. List USD
PCR Rack, 10 pcs. 0030124545 $187.00
4 Heat Sealing Materials

4 Adapters
212 Sample Handling
in situ Adapter
Technical specifications
Sample capacity Max. 4 glass slides 76 x 26 mm
Temperature range 4–99 °C
Temperature distribution 20–72 °C ±0.6 °C
across the adapter
95 °C
±1.0 °C
Temperature control speed ≥1.0 °C/s (heating)
measured on the adapter ≥0.7 °C/s (cooling)
Temperature Validation System for Mastercycler Family
Ordering information
Description Catalog No. List USD
Temperature Validation System
(96-well probe included)
950008059 $4,799.00
Temperature sensor, 384-well 950008041 840.00
Order from your laboratory supply dealer.
Product appearance, specifications, and/or prices are subject to change without notice.
4 | Consumables | PCR
Product features
‡ Easy to handle
‡ Perfect fit for optimal heat transfer
‡ Software supported, easy to program, extremely flexible
‡ Can accommodate 4 standard glass slides
‡ Transparent covering hood—in situ frames trap moisture
(sold separately)
Description
The in situ Adapter can be used to convert your Mastercycler ®
or Mastercycler nexus to an in situ device as easily as if you
were to insert a PCR plate. And the quality of the heat transfer
is equally as high! With precise block homogeneity, the in situ
Adapter has room for up to four standard glass slides.
Ordering information
Description Catalog No. List USD
in situ Adapter
for Mastercycler nexus
and Mastercycler
950007052 $1,397.00
in situ Frames Please Inquire
Product features
‡ Single-well probe
‡ Sensor for 0.2 mL tube position
‡ Optional: sensor for 384-well block, sold separately
‡ Simple, rapid validation and calibration
‡ Autocalibration with Mastercycler software, version
2.10 or higher
‡ Can be third-party calibrated
Description
The Temperature Validation System for the entire Mastercycler,
Mastercycler nexus, Mastercycler ep and Mastercycler pro
families enables simple and rapid validation and calibration of
block temperature. The program for automatic measurement
and calibration is integrated into the thermal cycler software,
thus providing an accurate and precise system that meets all
documentation and detection requirements in the laboratory.

Temperature Verification System
Product features
‡ Verification of the temperature control of the thermoblock
and the heated lid in accordance with the appropriate
Eppendorf ® specifications
‡ Generation of certificates of conformity and of measuring
reports for the thermocyclers
‡ The temperature control of the thermoblock can be
adjusted automatically, if necessary
‡ Recording of a temperature profile of user defined
programs in 14 thermoblock positions
‡ Increased number of measuring points: 14 block
thermosensors and 1 lid thermosensor
‡ Temperatures of all positions are measured
simultaneously—short data acquisition time per cycler
Ordering information
Description
Temperature Verification System
Catalog No. List USD
includes 96-well sensor plate 6328000006 $37,114.00
includes 384-well sensor plate 6328000014 37,636.00
Sensor plate for Temperature Verification System
96-well 6328071000 11,814.00
384-well 6328072007 12,336.00
Sample Handling 213
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 | Consumables | PCR
Description
The Eppendorf Temperature Verification System can
perform a verification, and if necessary an adjustment,
of the Mastercycler ® pro, Mastercycler ep, Mastercycler nexus
and Mastercycler ep realplex instruments according to the
manufacturer’s specifications. This system can also record
temperature profiles and measure ramp rates to ensure that
the thermal cycler is always at its peak performance level.
The Eppendorf Temperature Verification System has 14
block thermosensors and 1 lid thermosensor, all positions
are measured simultaneously for rapid data acquisition.
4 Thermocycler Software

Services – PCR
44
214 Sample Service products Handling
Mastercycler® ep realplex Performance Plans and Services
Value of Service
From warranty plus Protection that provides a LED light source
for life and a 2-year system warranty, to service plans for
every lab need, our goal is to help you achieve reproducible
results over an extended period of time. Reliable quantification
is critical in qPCR and Eppendorf ® Service will keep your
instrument in peak performance.
Choose a plan that’s right for you and a certified Eppendorf
Specialist professional will handle it all. Extended Warranty
and Advantage Service protect your hardware investment
while any one of three Performance Plans will keep your
system fine tuned and in compliance to deliver results you
can count on.
Service offers—ep realplex
Performance Plans—ep realplex
Order direct from Eppendorf North America.
Product appearance, specifications, and/or prices are subject to change without notice.
Long-term benefits of performance plans:
‡ Confidence in results
‡ GLP compliance
‡ Assurance of performance over extended lifetime
Features of Eppendorf Performance Plans:
‡ Complete satisfaction and quality delivered by
Eppendorf experts
‡ Complete programs—easy to understand,
easy to budget, no surprises
‡ Complete documentation management—
everything you need to be in compliance
Description Model Catalog No. List USD
Extended Warranty Mastercycler ep realplex 2-color system 950020927 $2,900.00
Mastercycler ep realplex 4-color system 950020935 3,420.00
Advantage Repair Service Mastercycler ep realplex 2-color system 901520068 7,095.00
Mastercycler ep realplex 4-color system 901520070 8,679.00
Preventive
Certification
Description
Maintenance Certification
Plus
Catalog No. 950033017 950033025 950033037
List USD $1,330.00 $3,350.00 $5,920.00
Preventive Maintenance
The Preventive Maintenance is carefully designed to keep
the Mastercycler ep realplex in the best condition. The
services include the following—cleaning of the instrument’s
surface and block, a functional test of the thermoblock with
a temperature validation, as well as a full system check with
background calibration.
‡ ‡ ‡
Color Calibration
A dedicated color calibration protocol is carried out by the
service specialist. Based on this recalibration procedure the
last issued certificate is updated.
— ‡ ‡
Validation Assay
The validation assay is based on a qPCR run with
specifically designed primers and DNA templates to cover
all major performance aspects such as dynamic range and
repro ducibility. Based on this validation protocol the last
issued certificate is updated.
— — ‡

Mastercycler® Systems Services
Value of Service
From a 2-year system warranty that comes with the unit,
to Extended Warranties and Preventive Maintenance, we
offer options that will keep your Mastercycler running for
many cycles.
Choose an Extended Warranty or Advantage Service to
ensure optimal performance of your instrument while
Preventive Maintenance will keep the system running
smoothly for years to come.
Advantage Repair Service
An easy to use value packaged service option when a
repair is needed for systems not covered under a warranty
or extended warranty. All it takes is one call to our Service
Center and for a single fee we will repair the instrument,
complete a full preventive maintenance, repair anything
else we find, plus include a full year warranty on the whole
system, not just the repair. Instrument return shipment is
also included.
Service offers—Mastercycler systems
Sample Service products Handling 215
Description Model Catalog No. List USD
Extended Warranty Mastercycler personal 901510551 $ 560.00
Mastercycler 901510553 910.00
Mastercycler gradient 901510555 980.00
Mastercycler ep/pro Manual lid 901510556 1,075.00
Mastercycler ep Motorized lid 901510557 1,270.00
Mastercycler nexus Please Inquire
Advantage Repair Service Mastercycler 901520044 2,551.00
Mastercycler gradient 901520046 2,747.00
Mastercycler personal 901520048 1,568.00
Mastercycler ep/pro Manual lid 901520050 2,741.00
Mastercycler ep Motorized lid 901520052 3,388.00
Mastercycler ep/pro S Manual lid 901520054 3,135.00
Mastercycler ep S Motorized lid 901520056 3,801.00
Mastercycler ep/pro 384 Manual lid 901520058 3,018.00
Mastercycler ep 384 Motorized lid 901520060 3,684.00
Mastercycler ep/pro Control Panel 901520061 892.00
Performance Plans—Mastercycler Mastercycler systemsnexus
Please Inquire
Description Model Catalog No. List USD
Preventive Maintenance Mastercycler Systems 901510526 $395.00
Temperature Validation
and Calibration
Mastercycler Systems 901510697 240.00
In the U.S.: Eppendorf North America 800-645-3050 · In Canada: Eppendorf Canada Ltd. 800-263-8715
E-mail: info@eppendorf.com · www.eppendorf.com
4 Services – PCR

Support and Services Directory
Contact Information
United States Canada
Business Hours: 8:30 a.m. to 8:00 p.m. EST 8:30 a.m. to 5:00 p.m. EST
Phone: 800-645-3050
516-334-7500
800-263-8715
905-826-5525
Fax: 516-334-7506 905-826-5424
Website: www.eppendorfna.com www.eppendorf.ca
Email: info@eppendorf.com canada@eppendorf.com
Customer Support: 800-645-3050
custserv@eppendorf.com
Technical Service: 800-645-3050
techserv@eppendorf.com
www.eppendorf.com • Email: info@eppendorf.com
800-263-8715
canadacustserv@eppendorf.com
800-645-3050
techserv@eppendorf.com
In the U.S.: Eppendorf North America, Inc. 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715