F-062726 GLutamax Flyer.indd - Invitrogen

What’s the ammonia level in your cell culture?

Minimizing toxic ammonia build-up with GlutaMAX
Media and Supplement (Figure 1) will:
• Maximize cell performance
• Improve cell viability and growth
• Extend cell culture life
Figure 1 — More stable GlutaMAX-I Supplement results in less ammonia in cell culture
compared to L-glutamine
A. Stability of GlutaMAX-I Supplement vs. L-Glutamine
900
800
700
600
500
mg/l 1000
400
300
200
A. D-MEM was supplemented with GlutaMAX-I Supplementor L-glutamine,
aliquoted into vials, and stored at 37˚C. Samples were taken daily and frozen
at -20˚C. Levels of GlutaMAX-I and L-glutamine were determined by HPLC.
Stabilize your media
L-glutamine is an essential, however unstable, component of most cell
culture media that decomposes spontaneously in aqueous, neutral
solutions (Figure 1A). This decomposition occurs much more rapidly at
37°C. This leads to ammonia build-up and cell degradation (Figure 1B). To
protect valuable cultures, substitute GlutaMAX-I Supplement directly
for L-glutamine. This stable, dipeptide form of L-glutamine eliminates this
source of toxicity, even in long-term cultures.
Reduce media changes
GlutaMAX Supplement is only consumed from the medium by cell
metabolism. There is no build-up of toxic metabolites due to spontaneous
breakdown. This may reduce the number of times cells must be passaged.
Improve cell viability
Using GlutaMAX-I Supplement in place of L-glutamine in cell culture can
improve cell viability and growth * . Figure 2 shows an AE-1 cell growth curve
with the GlutaMAX-I culture demonstrating improved cell numbers.
FREE Sample
L-glutamine
GlutaMAX-I
Supplement
GlutaMAX-I Supplement
L-glutamine
0 1 2 3 4 5 6 7
Day
B. Ammonia Increase at 37°C - GlutaMAX-I
Supplement vs. L-Glutamine
L-glutamine
GlutaMAX -I
Supplement
L-glutamine
To receive a FREE sample or to place an order, visit www.invitrogen.com/glutamax today.
* Results vary depending on cell line.
Ammonia (mg/l)
70
60
50
40
30
20
10
0
GlutaMAX -I Supplement
0 1 2 3 4 5 6 7
Day
B. D-MEM was supplemented with GlutaMAX-I Supplement or L-glutamine,
aliquoted into vials and stored at 37˚C. Samples were taken daily and
frozen at -20˚C. Levels of ammonia were determined by HPLC.
Figure 2 — Improved cell growth in the GlutaMAX-I culture
AE-1 mouse myeloma cells were seeded at 1 x 105 cells/ml. Samples were
taken daily after three days and evaluated in triplicate for cell density. Cell
viability was determined by trypan blue exclusion. Cell density continued to
increase in samples containing GlutaMAX-I Supplement past the point when
L-glutamine- containing samples decreased in cell density.
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F-062726-r1 US 0805 0M