Complete Set-up Information for Method 1623 - Invitrogen
Complete Set-up Information for Method 1623 - Invitrogen
Complete Set-up Information for Method 1623 - Invitrogen
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<strong>Complete</strong> <strong>Set</strong>-<strong>up</strong> <strong>In<strong>for</strong>mation</strong> <strong>for</strong> <strong>Method</strong> <strong>1623</strong><br />
Please read through the package insert and EPA <strong>Method</strong> <strong>1623</strong> be<strong>for</strong>e using the test kit.<br />
Below is a summary of the s<strong>up</strong>plies that are recommended to per<strong>for</strong>m <strong>Method</strong> <strong>1623</strong>*:<br />
Filtering and appropriate apparatus <strong>for</strong> elution and concentration:<br />
IDEXX:<br />
1-888-433-9987 or 1-800-321-0207<br />
www.idexx.com<br />
PALL Gelman Laboratory:<br />
1-800-521-1520 or 734-913-6197<br />
www.pall.com/gelman<br />
Immunomagnetic Separation Materials:<br />
www.dynalbiotech.com<br />
Hardware required <strong>for</strong> IMS s<strong>up</strong>plied by Dynal Biotech ® :<br />
Catalogue Number Description<br />
947.01 Dynal ® Sample Mixer (US Only)<br />
159.07 Dynal MX-1 (International 220V)<br />
120.01 Dynal ® (Magnetic Particle Concentrator) MPC ® -1**<br />
120.02 Dynal MPC ® -6 **<br />
120.20 Dynal MPC ® -S ***<br />
740.03 Dynal ® L10 Tubes (5 tubes, re-usable)<br />
Consumables required <strong>for</strong> IMS s<strong>up</strong>plied by Dynal Biotech ® :<br />
Catalogue Number Description<br />
730.02 Dynabeads ® GC Combo (10 tests)<br />
730.12 Dynabeads® GC Combo (50 tests)<br />
740.04 Dynal® Spot-On Slides (100/package)<br />
A CD-ROM of the whole IMS procedure is available to users. To request a CD please e-mail<br />
ukcusterv@dynabiotech.com
Additional material required <strong>for</strong> IMS, not s<strong>up</strong>plied by Dynal Biotech ® :<br />
(Available through standard scientific s<strong>up</strong>ply companies)<br />
• Test tubes, glassware, Pasteur pipettes<br />
• Vortex type mixer<br />
• Micro-pipette (10-1000uL) and tips<br />
• Humid Chamber<br />
• Incubator<br />
• Reagent grade, oocyst-free water<br />
• 0.1N hydrochloric acid (HCL)<br />
• 1.0N sodium hydroxide (NaOH)<br />
• Microcentrifuge tubes (round-bottomed, polypropylene, 1.5mL)<br />
• DABCO/glycerol mounting medium<br />
• Cover-slips<br />
• Fluorescent Microscope with appropriate filters<br />
Stains (FITC/DAPI) and Control Materials:<br />
Biotechfrontiers:<br />
+61 2 8877 9150<br />
www.biotechfrontiers.com<br />
Meridian Bioscience, Inc.:<br />
1-800-543-1980 or 513-271-3700<br />
www.meridianbioscience.com<br />
WaterBorne:<br />
(504)-895-3338<br />
www.waterborneinc.com<br />
Wisconsin State Laboratory of Hygiene:<br />
206-224-6202<br />
www.slh.wisc.edu<br />
*Please note: this is a summary list <strong>for</strong> your convenience, additional s<strong>up</strong>pliers may be available. Dynal Biotech ®<br />
does not endorse any of these companies nor are they responsible <strong>for</strong> incomplete, incorrect or outdated<br />
in<strong>for</strong>mation and/or per<strong>for</strong>mance issues or concerns with products outside of the Dynal ® brand.<br />
**It is not necessary to purchase both of these concentrators. The MPC ® -1 holds (1) L10 tube at a time and can<br />
be used repeatedly to do multiple samples. The MPC ® -6 holds (6) L10 tubes at once. It is necessary to have an<br />
MPC ® -1 or MPC ® -6, as well as, a MPC ® -M or MPC ® -S.<br />
***This magnet replaces the MPC ® -M. The MPC ® -S holds 6 microcentrifuge tubes, has newly designed clips to<br />
hold the tubes securely in place, incorporates stronger magnets and utilizes tighter manufacturing specifications<br />
to decrease magnetic variance across the magnet.
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