6 What’s the Worst That Could Happen? Environmental Impact <strong>Results</strong> (USA) Conclusion SYBR Safe DNA gel stain is not classifi ed as corrosive, ignitable, or reactive under the guidelines <strong>of</strong> the Environmental Protec- tion Agency (EPA). Table 3. SYBR Safe stain—Hazardous waste analysis Analysis * Method <strong>Results</strong> Corrosivity EPA 150.1 Not corrosive (pH = 8.25) Corrosivity (by Corrositex) DOT-E 10904 Category 2 noncorrosive Ignitability EPA 1010 Not ignitable (
Conclusion SYBR Safe DNA gel stain in 0.5X TBE is indistinguishable from 0.5X TBE alone in terms <strong>of</strong> organic pollutant content—both samples tested negative for the presence <strong>of</strong> an extensive array <strong>of</strong> organic compounds. Table 5. SYBR Safe stain—Detailed organic compound analysis * Organochlorine pesticides <strong>and</strong> PCBs (608M) alpha-BHC beta-BHC gamma-BHC delta-BHC (Lindane) Heptachlor Aldrin Heptachlor Epoxide Endosulfan I Dieldrin 4,4’-DDE Important Licensing Information These products may be covered by one or more Limited Use Label Licenses (see the <strong>Invitrogen</strong> website, www.invitrogen.com, or the Molecular Probes website, probes.invitrogen.com). By use <strong>of</strong> these products you accept the terms <strong>and</strong> conditions <strong>of</strong> all applicable Limited Use Label Licenses. All products are for research use only. CAUTION: Not intended for human or animal diagnostic or therapeutic uses. SYBR Safe DNA Gel Stain Endrin Endosulfan II 4,4’-DDD Endrin Aldehyde Endosulfan Sulfate 4,4’-DDT Toxaphene Chlordane Aroclor 1016 Aroclor 1221 Aroclor 1232 Aroclor 1242 Aroclor 1248 Aroclor 1254 Aroclor 1260 Ethylbenzene Brom<strong>of</strong>orm 1,1,2,2,-Tetrachloroethane 1,3-Dichlorobenzene Volatile organic compounds (624) Chloromethane Vinyl Chloride Bromomethane Chloroethane Trichlor<strong>of</strong>l uoromethane 1,1-Dichloroethene Methylene Chloride trans-1,2-Dichloroethene 1,1-Dichloroethane Chlor<strong>of</strong>orm 1,1,1-Trichloroethane (TCA) Carbon Tetrachloride Benzene 1,2-Dichloroethane (EDC) Trichloroethene (TCE) 1,2-Dichloropropane Bromodichloromethane 2-Chloroethyl Vinyl Ether trans-1,3-Dichloropropene Toluene cis-1,3-Dichloropropene 1,1,2-Trichloroethane Tetrachloroethene (PCE) Dibromochloromethane Chlorobenzene 1,4-Dichlorobenzene 1,2-Dichlorobenzene Acrolein Acrylonitrile Semi-volatile organic compounds (625) N-Nitrosodimethylamine Bis(2-chloroethyl) Ether Phenol 2-Chlorophenol Bis (2-chloroisopropyl) Ether Hexachloroethane N-Nitrosodi-n-propylamine Nitrobenzene Isophorone 2-Nitrophenol 2,4-Dimethylphenol 4-Nitrophenol 2,4-Dichlorophenol 1,2,4-Trichlorobenzene Naphthalene Hexachlorobutadiene 4-Chloro-3-methylphenol Hexachlorocyclopentadiene 2,4,6-Trichlorophenol 2-Chloronaphthalene Acenaphthylene Dimethyl Phthalate 2,6-Dinitrotoluene Acenaphthene 2,4-Dinitrophenol Bis (2-chloroethoxy) methane 2,4-Dinitrotoluene Fluorene Diethyl Phthalate 2-Methyl-4,6-dinitrophenol N-Nitrosodiphenylamine 4-Bromophenyl Phenyl Ether Hexachlorobenzene Pentachlorophenol Phenanthrene Anthracene Di-n-butyl Phthalate Fluoranthene Benzidine Pyrene Butyl Benzyl Phthalate 3,3’-Dichlorobenzidine Benz(a)anthracene Chrysene Bis(2-ethylhexyl) Phthalate Di-n-octyl Phthalate Benzo(b)fl uoranthene Benzo(k)fl uoranthene Benzo(a)pyrene Indeno(1,2,3-cd)pyrene Dibenz(a,h)anthracene Benzo(g,h,I)perylene 1,2-Diphenylhydrazine 2,3,7,8-Tetrachlorodibenzo-p-dioxin 4-Chlorophenyl Phenyl Ether * Samples analyzed were 0.5X TBE <strong>and</strong> 0.5X TBE + 1X SYBR Safe stain; none <strong>of</strong> the compounds listed in the table were detected in either sample. <strong>Test</strong>ing was performed by Columbia Analytical Services, Inc., Kelso, WA. Methods used were as outlined in the Code <strong>of</strong> Federal Regulations (CFR) Title 40, Part 136. w w w.invitrogen.com | probes.invitrogen.com 7